Scapania gigantea Horik.

Kuznetsova, O. I., Bakalin, V. A., Konstantinova, N. A., Laphina, E. D., Ganasevich, G. N., Potemkin, A. D. & Vilnet, A. A., 2022, Bryophyte Molecular Barcoding Records. 6, Arctoa 31 (1), pp. 59-61 : 59-60

Scapania gigantea Horik.

Contributors: A.D. Potemkin, A.A. Vilnet, V.A. Bakalin 2

Specimen: Russia, Sakhalin Province, northern part of Iturup Island, Tsirk Bay, Tsirk River lower course, 45°21’26”N, 148°37’11”E, 20 m a.s.l., sedge-peat moss wetland in the flood plain, wet stone near stream, 12.IX.2015, V.A. Bakalin & E.A. Borovichev K-72-25- 15, det. V V.A. Bakalin as Scapania uliginosa (Lindenb.) Dumort. and distributed in Hepaticae Rossicae Exsiccatae Fasc. IX. No. 300. Revised by Potemkin, Vilnet and Bakalin as Scapania gigantea . (LE, VBGI, KPABG).

GenBank accession numbers are OM501133 for ITS1- 2 nrDNA and OM515128 for trn L-F cpDNA .

The ITS1-2 nrDNA and trn L-F cpDNA nucleotide sequences for specimen from Iturup Island were obtained according with protocols described in Potemkin et al. (2021). The test of genetic distances in MEGA 11 ( Tamura et al., 2021) suggested ITS1-2 nucleotide sequence divergence of the Iturup specimen from accessions of Scapania gigantea from Kunashir Island ( MZ272035 ) in 0.9% and form Shikotan ( MZ272036 ) – in 0.8%. The trn L-F sequence variability consists 0.4% with accession from Honshu ( MZ274288 ), 0.2% with accession from Shikotan ( MZ274290 ) and absent with accession from Kunashir ( MZ274289 ). The new record increases infraspecific sequence variation in Scapania gigantea compared with previously published 0.6% in ITS1-2 and 0.1% in trn L-F (Potemkin et al., 2021), but agrees with level of infraspecific variation in the genus Scapania ( Bakalin et al., 2019) .

Distribution and habitat. Previously Scapania gigantea was reported from Kunashir, Shikotan, and Honshu Islands, and the Yunnan Province of China. In all

1 – Tsitsin Main Botanical Garden, Russian Academy of Sciences, Botanicheskaya Str., 4, Moscow 127276 Russia. E-mail: [e-mail]; ORCID (OK): 0000-0002-5513-1329.

2 – Botanical Garden-Institute, Makovskogo Str., 142, Vladivostok, 690024 Russia. E-mail: [e-mail]. ORCID: 0000- 0001-7897-4305.

3 – Polar-Alpine Botanical Garden-Institute of the Russian Academy of Sciences, Akademgorodok, Fersmana Str., 18A, Apatity, Murmansk Province, 184209 Russia. E-mails: e-mails: [e-mail]; [e-mail]. ORCID: (NK) 0000-0002-7600- 0512; (AV) 0000-0001-7779-2593;

4 – Yugra State University, 16 Chekhov St., Khanty-Mansiysk, 628012 Russia – Россия 628012, г. Ханты-Мансийск, ул. Чехова, д. 16, Югорский государственный университет; e-mail: [e-mail]

5 – Komarov Botanical Institute, Professor Popov Str. 2, St. Petersburg, 197376 Russia. E-mail: [e-mail]. ORCID: (AP) 0000-0003-4420-1704

known locations, it grows on rocks and sandy soil in mountain streams and on their banks (Potemkin et al., 2021). This record of Scapania gigantea is the first for Iturup Island.

Taxonomic notes. Despite Scapania gigantea was not accepted in the World checklist of hornworts and liverworts ( Söderström et al., 2016), and in the Checklist of Japanese liverworts and hornworts ( Katagiri & Furuki, 2018) after its resurrection by Potemkin (2001), it’s species status was confirmed by Potemkin et al. (2021).

Scapania gigantea from Itutup Island is distinct in the large plants, 4–6 cm long, deep purplish-black color, and characteristic development of teeth at and near the decurrent strip of the dorsal lobe.

2. Rudolgaea borealis (Frisvoll & Moen) Potemkin & Vilnet [ Gymnocolea borealis (Frisvoll et Moen) R.M.Schust. ]

Contributors: N.A. Konstantinova, E.D. Lapshina, A.A. Vilnet, G.N. Ganasevich

Specimen: Russia, Krasnoyarsk Territory, the southern part of the Taimyr Peninsula, southern tundra subzone in the middle reaches of the Dudypta River between the mouths of the Bataika and Avam rivers, sedge-hypnum floating fen on the shore of thermokarst lake with Carex chordorriza , C. rotundata , 38 m above sea level, 71.23266°N, 92.66041°E, single shoots in mats dominated by Scorpidium revolvens (Sw. ex anon.) Rubers with admixture of Oncophorus demetrii (Renauld et Cardot) Hedenas , Cinclidium subrotundum Lindb. , Warnstorfia sarmentosa (Wahlenb.) Hedenäs. Coll. G.N. Ganasevich. YSU #4354. The plants in the specimen are large (to 2 mm wide) and warm dark brown in color, which is somewhat different from the description of the species as green to yellow and golden brown.

GenBank accession numbers are ON175945 for ITS1- 2 nrDNA, ON185621 for trn L-F cpDNA .

The nucleotide sequences comparison between specimens from Gydansky ( MZ343174, MZ353627 ) and Taimyr Peninsulas resolved in identity of reference loci .

The species was described as Lophozia subg. Leiocolea (Frisvoll & Moen, 1980) , but then transferred to Gymnocolea ( Schuster, 1986) and, finally, as a result of molecular phylogenetic estimation, isolated into a distinct genus Rudolgaea (Potemkin & Vilnet, 2021) . Until recently, the species was recorded for Fennoscandia, where it is not uncommon as well as for Greenland ( Damsholt, 2002). For Russia, R. borealis was first recorded just last year for Gydansky Peninsula (Potemkin et al., 2021). Given the narrow ecological amplitude of the species and the occurrence of usually as individual shoots in the mats of mosses it can be assumed that the species overlooked during collecting and identification and has in fact circumpolar distribution, similar to distribution of rich fen mosses communities.

3. Solenostoma rossicum Bakalin & Vilnet

Contributors: N.A. Konstantinova, E.D. Lapshina, A.A. Vilnet

Specimen: Russia, Yamal-Nenets Autonomous Area, Nadymsky District, 40 km north of Pangody town, on the border of forest tundra and southern tundra subzones; Larix sibirica -Salix -Polytrichum -lichen tundra, on patches of bare clay soil, with admixture of Blasia pusilla , Cephalozia bicuspidata, Nardia sp., Schistochilopsis incisa , Scapania obcordata , Solenostoma cf. pusillum , 66.06586°N, 74.04234°E. Coll. E.D. Lapshina, 5VIII.2019 #378E/3-1-19, KPABG 124440.

GenBank accession numbers are ON185619 for trn L- F cpDNA, ON185620 for trn G-intron cpDNA .

The nucleotide sequence divergence of newly sequenced samples (0-0.5% in trn L-F, 0% in trn G-intron as calculated in Mega 11 ( Tamura et al., 2021)) fits well in level of infraspecific variability ( Kusnetsova et al., 2020).

Solenostoma rossicum is a recently described ( Bakalin & Vilnet, 2012 as « rossica») but not universally accepted species ( Váňa et al., 2013; Söderström et al., 2016). As it turns out in the course of our work, the species differs quite well not only at the nucleotide sequence data, but also in appearance ( Kusnetsova et al., 2020). Based on the discovery of the species in the Khanty-Mansi Autonomous Area (l. c.), on the mountain pass of eastern slope of Subpolar Urals (65.21085°N, 61.97035°E) and taking into account the ecology of the species occurring on bare clay soil and, in particular, on mud boils and non-sorted circles in tundra, we assumed its much wider, possibly circumpolar distribution. The discovery of the species in the Yamal-Nenets Autonomous Area confirms this assumption.