Dna extraction

Duran, Ahmet, Lyskov, Dmitry, Samigullin, Tahir & Dündar, Ekrem, 2025, Pimpinella husnucan-baseri (Apiaceae), a new species from South Anatolia, Turkey, Phytotaxa 702 (1), pp. 29-47 : 30

DNA extraction , nuclear ribosomal ITS amplification and sequencing

Total genomic DNA was isolated from fruit material using a Diamond DNA Plant kit (ABT, Barnaul, Russia) following the manufacturer’s instructions. Polymerase chain reaction (PCR) amplification was performed on a Biometra T3000 Thermocycler using an Encyclo PCR kit (Evrogen JSC, Moscow, Russia). Details of the PCR nrITS region amplifications (including primer locations and characteristics) and sequencing strategies for nrITS were described by Valiejo-Roman et al. (2002). Cycle sequencing was performed by automated sequencing using Big Dye Terminator version 3.1 for both forward and reverse strands was conducted on ABI Prism 3100-Avant (Applied Biosystems). Newly obtained sequences were deposited in the GenBank (Appendix 1).