Cucumis sativus

Zandi, Peiman, Możdżeń, Katarzyna, Barabasz-Krasny, Beata & Wang, Yaosheng, 2019, The role of magnesium salts in germination and growth of CUCUmiS SAtivUS L., Annales Universitatis Paedagogicae Cracoviensis Studia Naturae 4, pp. 119-131 : 120-121

Cucumis sativus seeds from POLAN company were used in the experiment. Kottke

et al. (1987) medium and their modi cations were used for the experiments:

[1] – pH 5.4, [2] with the addition of MgSO 4 ×7H 2 O – pH 4.8, [3] with the addition of MgCO 3 ×3H 2 O – pH 6.5. ffle control was performed on distilled water [4].

Germination

ffle cleaned seeds, in a 1% solution of acetone and washed with running and distilled water, were put 25 pieces with tweezers on sterile Petri dishes with a triple layer of lter paper moistened with a suitable type of medium and distilled water (control). ffle Petri dishes with seed were placed in the dark, at room temperature. Every 24 hours the number of germinated seeds was checked. Germinated seeds were considered to be those with sprouts equal to at least 2 mm. A er 7 days, germination capacity of cucumber seeds were measured by germination indexes according to Islam and Kato-Noguchi (2014): germination energy ( GE), germination as a percentage of control ( G %), speed emergence ( SE), mean germination time ( MGT) were measured .

Growth of plants

A er 3 days, germinated seeds were rinsed with distilled water and planted into pots with sand (10 plants for each series). Plants were grown in a growth chamber (Angelantoni Industrie, Italy) in a 12 / 12h photoperiod, at 25°C / 20°C (day / night) and relative humidity RH% 70–90%.

Biometric analysis

A er 27 days, the length of underground and aboveground organs of cucumber plants was analysed. An inhibition percentage of growth (IP) index for root, hypocotyl, stalk, petioles was measured according to method used by Islam and Kato-Noguchi (2012).

Fresh, dry mass and turgor water content

Fresh mass of organ plants (root, hypocotyl, stalk, petiole, cotyledons, rst leaves, second leaves) were measured on scale (Radwag WPS 120, Poland). Turgor water content (TWC) according to ( Mullan, Pietragalla, 2012) was checked. Dry mass after 48 h of drying plant material in dryer (Wamed SUP 100, Poland) at temperature 105°C was measured.

Detailed analyses concerned the assessment of the e ect of nutrients on germination, growth on the length, weight increase and water content of cucumber specimens: grown from seeds germinated in distilled water, and watered of the media during growth [I] and specimens grown from seeds germinated on media and also in the growth stage watered with media [II].

Statistical analysis

ffle germination experiment was carried out in two series by 25 seeds on Petri dishes for each Kottke medium modi cation and distilled water. In growth stage in two repetition,10 plants for each treatment of medium were measured. ffle results were analysed by the ANOVA / MANOVA parametric test using the post hoc Duncan test p <0.05.