Dna extraction, , PCR

Yang, Kun L., Lin, Jia Y., Li, Guang-Mei & Yang, Zhu L., 2025, New and interesting fungi from Guangzhou City, South China, Phytotaxa 707 (1), pp. 1-95 : 6

DNA extraction, PCR , and sequencing

Genomic DNA was extracted from tiny fragments of the collections by using the Ezup Column Fungi Genomic DNA Purification Kit (Sangon Biotech Co.) . Loci for phylogenetic analyses were selected following the recent usage in certain groups, including the nuclear internal transcribed spacer region (ITS), the nuclear large subunit rDNA (nrLSU), the laccase gene (lac3-1), the RNA polymerase II largest subunit gene (rpb1), the RNA polymerase II second largest subunit gene (rpb2), the translation elongation factor 1 alpha gene (tef-1α) and the beta tubulin gene (β-tub), amplified with the primer pairs and specific PCR settings listed in Table 1. All reactions were proceeded with a pre-denaturation at 94 °C for 3 min and a final elongation at 72 °C for 8 min. PCR products were purified and sequenced by Sangon Biotech Co. Raw sequences were checked and trimmed with Chromas v2.6.6 ( Technelysium 2025) and assembled with AliView v1.28 ( Larsson 2014, 2021). Assembled sequences were deposited in GenBank ( NCBI 2025) with the accession numbers shown in Tables 2–16.