Dna extraction, , PCR

Yang, Kun L., Lin, Jia Y., Li, Guang-Mei & Yang, Zhu L., 2025, New and interesting fungi from Guangzhou City, South China, Phytotaxa 707 (1), pp. 1-95 : 6

DNA extraction, PCR , and sequencing

Genomic DNA was extracted from tiny fragments of the collections by using the Ezup Column Fungi Genomic DNA Purification Kit (Sangon Biotech Co.) . Loci for phylogenetic analyses were selected following the recent usage in certain groups, including the nuclear internal transcribed spacer region (ITS), the nuclear large subunit rDNA (nrLSU), the laccase gene ( lac3-1), the RNA polymerase II largest subunit gene ( rpb1), the RNA polymerase II second largest subunit gene ( rpb2), the translation elongation factor 1 alpha gene ( tef-1α) and the beta tubulin gene ( β-tub), amplified with the primer pairs and specific PCR settings listed in Table 1. All reactions were proceeded with a pre-denaturation at 94 °C for 3 min and a final elongation at 72 °C for 8 min. PCR products were purified and sequenced by Sangon Biotech Co. Raw sequences were checked and trimmed with Chromas v2.6.6 ( Technelysium 2025) and assembled with AliView v1.28 ( Larsson 2014, 2021). Assembled sequences were deposited in GenBank ( NCBI 2025) with the accession numbers shown in Tables 2–16.