Stenostola ferrea

Kvamme, Torstein, Wallin, Henrik & Kvie, Kjersti S., 2012, Taxonomy and DNA barcoding of Stenostola ferrea (Schrank, 1776) and S. dubia (Laicharting, 1784) (Coleoptera, Cerambycidae, Saperdini), Norwegian Journal of Entomology 59, pp. 78-87 : 81

Stenostola ferrea

Aedeagus: approx. 1.5 mm long, slender, narrowed and weakly curved towards the very sharp, pointing apex, dorsal ridge as wide as

ventral ridge. The crescent-shaped sclerites at the proximal end of the basal segment consist of four separate parts (two rectangular and two crescent-shaped) ( Figure 2 ), and three elongated median sclerites (approx. 1.8 mm long) inside the internal sac forming a slightly bent thread with the posterior end most narrow and not straight: forming an irregular curved or “zigzag” shape ( Figure 3 ); surrounding intersegmental membrane with very fine, speckled microreticulation. Tegmen: approx. 1.6 mm long, parameres slender and flattened dorsoventrally, less separated medially along inner margin and towards apex forming a “V-shaped” cavity. Apex evenly rounded along entire posterior margin, with fringes of relatively long, yellowish hairs well concentrated at edge of apex. No microreticulation on parameres. Base of tegmen, at lower part of median lobe, extended and weakly curved dorsoventrally on middle. Tergite VIII: approx. 0.5 mm long, with fine, brownish pigmentation, rounded at the posterior margin with a concave or notched part medially, and covered with short, very fine yellowish hairs distally towards the posterior margin. Surface has weak micro-reticulation medially. Spermatheca: approx. 0.12 mm wide, round and brownish ( Figure 4 ).

Identification of larvae

DNA results

Four out of five extracted samples were successfully DNA barcoded. Two attempts to extract DNA from different parts of the Swedish pupa of S. dubia failed, it is possible a different method is necessary for pupae. The results from the neighbour-joining analysis show that S. ferre a and S. dubia cluster into two distinct groups ( Figure 12 ). The genetic distance between S. ferrea and S. dubia COI-haplotypes is 10.3 %.

Figures

FIGURE 2. The crescent-shaped sclerites at the proximal end of the basal segment of Stenostola dubia (Laicharting, 1784) (left) and S. ferrea (Schrank, 1776) (right) (Photo H. Wallin).

FIGURE 3. The elongated median sclerites inside the internal sac of Stenostola dubia (Laicharting, 1784) (above) and S. ferrea (Schrank, 1776) (below) (Photo H. Wallin).

FIGURE 4. The crescent-shaped sclerites at the proximal end of the basal segment of Stenostola dubia (Laicharting, 1784) (left) and S. ferrea (Schrank, 1776) (right) (Photo H. Wallin).

FIGURE 12. Neighbour-joining analysis of Stenostola ferrea (Schrank, 1776), S. dubia (Laicharting, 1784) and Phytoecia algerica Desbrochers des Loges, 1870, COI sequences. S. ferrea and S. dubia cluster into two distinct groups with 10. 3 % genetic distance. P. algerica (sequence downloaded from GenBank with accession number AM283245.1) is included as an outgroup. Bootstrap support is shown at each node.