Eichleriella yunnanensis Y.L. Deng & C.L. Zhao, 2024

Deng, Yinglian, Chen, Meng, Liu, Linfeng, Zhao, Rui, Li, Qi, Miao, Jiayun, Zhao, Changlin & Zhou, Hongmin, 2024, Molecular phylogeny and morphology reveal a new Eichleriella species (Auriculariales, Basidiomycota) from China, Phytotaxa 675 (3), pp. 247-260 : 254-257

Eichleriella yunnanensis Y.L. Deng & C.L. Zhao , sp. nov. Figs. 3 and 4

MycoBank no.: MB 854309

Etymology: — yunnanensis (Lat.) : referring to the locality (Yunnan Province) of the new species.

Holotype: — CHINA. Yunnan Province, Zhaotong, Wumengshan National Natural Reserve , 27°51′N, 104°22′E, elev. 2300 m, on the fallen branch of angiosperm, 25 August 2023, CLZhao 31317 ( SWFC) .

Basidiomata: —Annual, resupinate, ceraceous, without odor or taste when fresh, becoming hard brittle upon drying, very hard to separate from substrate, first as small circular to orbicular colonies, later confluent, up to 18 cm long, 4 cm wide, 100–300 μm thick. Hymenial surface grandinioid, cream (4A2/3) to flesh-pink (8A3/9A4) when fresh, turning to buff (4A4) to clay-pink (6B/C4) upon drying. Sterile margin thin, slightly cream, up to 1 mm.

Hyphal structure: —Hyphal system monomitic, generative hyphae with clamp connections, colorless, thin- to thick walled, interwoven, 1.5–4 μm in diameter; IKI-, CB-, tissues unchanged in KOH.

Hymenium: —Cystidia and cystidioles absent. Dendrohyphidia arising from generative hyphae, nodulose or branched, colorless, thin-walled, 1–3.5 μm in diameter. Basidia subglobose to subclavate, longitudinally septate, two to four-celled, thin-walled, 10–35 × 6–10 µm, with oil drops; basidioles subglobose, similar to basidia in shape, but slightly smaller.

Spores: —Basidiospores curved-cylindrical to allantoid, hyaline, smooth, thin-walled, with 1–2 oil drops, IKI-, CB-, (6.5–)7.5–11.5(–12) × (3–)3.5–5(–6) µm, L = 9.53 μm, W = 4.06 µm, Q = 2.04–2.69 (n = 300/10).

Type of rot: —White rot

Additional specimens examined (paratypes): — CHINA. Yunnan Province, Zhaotong, Wumengshan National Natural Reserve , 27°51′N, 104°22′E, elev. 2300 m, on the branch fallen of angiosperm, 25 August 2023, CLZhao 31325, CLZhao 31350, CLZhao 31354, CLZhao 31364, CLZhao 31369, CLZhao 31372, CLZhao 31376, CLZhao 31437, CLZhao 31440, CLZhao 31441, CLZhao 31456, CLZhao 31479, CLZhao 31496, CLZhao 31514, CLZhao 31525, CLZhao 31527, CLZhao 31537, CLZhao 31545, CLZhao 31550, CLZhao 31554, CLZhao 31556, CLZhao 31560, CLZhao 31563 ( SWFC) ; 26 August 2023, CLZhao 31606, CLZhao 31628, CLZhao 31681, CLZhao 31688 ( SWFC) , 27 August 2023, CLZhao 31854, CLZhao 31870, CLZhao 31872, CLZhao 31875, CLZhao 31929, CLZhao 31943, CLZhao 32002 ( SWFC) ; on the stump of angiosperm, 27 August 2023, CLZhao 31940, CLZhao 31963 ( SWFC) ; on the trunk of angiosperm, 28 June 2023, CLZhao 32461 ( SWFC) ; on the branch fallen of angiosperm, 29 August 2023, CLZhao 32743, CLZhao 32762, and CLZhao 32845 ( SWFC) ; on the trunk of angiosperm, 29 June 2023, CLZhao 32797 ( SWFC) ; on the branch fallen of angiosperm, 20 September 2023, CLZhao 33416, CLZhao 33431, and CLZhao 33486 ( SWFC) .

Notes: —The phylogenetic tree ( Figures 1 and 2 ) inferred from ITS and nLSU sequences revealed that Eichleriella yunnanensis was nested into the genus Eichleriella s.s. within the family Auriculariaceae , in which E. yunnanensis formed a monophyletic lineage, and it was then grouped closely with two taxa E. discolor and E. xinpingensis from China. However, E. discolor can be delimited from E. yunnanensis by having spiny-to-velutinous from hyphal pegs, with smooth areas between pegs, hymenial surface first yellowish white (4A2), pale yellow (4A3), orange white (5A2), pale orange (5A3), light orange (5A4), then darkening to greyish orange (5B3), brownish orange (5C5), yellowish brown (5D4), thin-walled generative hyphae, ellipsoid to ovoid basidia, and larger cylindrical basidiospores (11–13.5 × 5–6 µm), often ventrally depressed ( Li et al. 2023). The species E. xinpingensis can be distinguished from E. yunnanensis by its flesh-pink to clay-pink to vinaceous hymenial surface covered by blunt-pointed spines, narrowly ovoid to obconical basidia with two celled, and broadly cylindrical basidiospores ( Liu et al. 2019).

Furthermore, Eichleriella yunnanensis resembles E. aculeobasidiata and E. xinpingensis in sharing cylindrical to allantoid basidiospores. Howerver, E. aculeobasidiata can be distinguished from E. yunnanensis by having dimitic hyphal system with clamped generative hyphae, and larger allantoid basidiospores (9–13.5 × 4.5–7.5 µm, Wang et al. 2022). The taxa E. xinpingensis differs from the new species by its blunt-pointed spines hymenial surface, dimitic hyphal system ( Liu et al. 2019).

Eichleriella yunnanensis shares similarities with Heterocorticium bambusicola S.H. He, T. Nie & Yue Li (2023: 7) and H. latisporum S.H. He, T. Nie & Yue Li (2023: 10) in sharing monomitic hyphal system with clamped generative hyphae. Howerver, morphologically, Heterocorticium bambusicola differs from E. yunnanensis by its wider ovoid to subglobose basidia with four-celled, without enucleate stalk (13–20 × 9–11 µm), and larger cylindrical basidiospores with a distinct apiculus (10–14 × 5–6 µm, Li et al. 2023); H. latisporum can be different from E. yunnanensis by having wider ovoid to subglobose, longitudinally septate basidia with four–celled (16–22 × 11–15 µm), and capable of germinating by repetition, broadly ellipsoid-to-ovoid basidiospores with a distinct apiculus (11–13 × 8–9 µm, Li et al. 2023). In addition, the new speices resembles Heteroradulum maolanense S.H. He, T. Nie & Yue Li (2023: 18) and H. yunnanense C.L. Zhao (2020: 57) in sharing cylindrical to allantoid basidiospores. However, H. maolanense differs from Eichleriella yunnanensis by its odontoid, irpicoid to subporoid from hyphal pegs hymenophore, monomitic-tosubdimitic hyphal system, longitudinally septate, ovoid to subglobose basidia with four-celled (18–25 × 10–15 µm), and capable of germinating by repetition, larger basidiospores with an apiculus (12–15 × 6–7.5 µm, Li et al. 2023). H. yunnanense can be different from E. yunnanensis by having odontoid hymenial surface, longitudinally septate and narrowly ovoid to obconical basidia with two to three-celled (28–41 × 9–14 µm), and larger basidiospores (17–24 × 5–8 µm, Guan et al. 2020).

Figures

FIGURE 1. Maximum parsimony strict consensus tree illustrating the phylogeny of two new species and related species inAuriculariaceae within Auriculariales based on ITS+nLSU sequences. The branch is labeled with a maximum likelihood lead value greater than 70%, a reduced lead value greater than 50% and a Bayesian posterior probability greater than 0.95. The new species are in bold.

FIGURE 2. Maximum parsimony strict consensus tree illustrating the phylogeny of the new species and related species in Eichleriella based on ITS sequences. The branch is labeled with a maximum likelihood lead value greater than 70%, a reduced lead value greater than 50% and a Bayesian posterior probability greater than 0.95. The new species are in bold.

FIGURE 3. Eichleriella yunnanensis (holotype, CLZhao 31317): basidiomata on the substrate (A), macroscopic characteristics of hymenophore (B). Bars: (A) = 1 cm and (B) = 1 mm.

FIGURE 4. Microscopic structures of Eichleriella yunnanensis (holotype, CLZhao 31317): basidiospores (A), basidia (B), basidioles (C), cystidia (D), hyphidia (E), a section of the hymenium (F). Bars: A–F= 10 µm.