Ameronothrus Berlese 1896

Pfingstl, Tobias, Hiruta, Shimpei F., Wagner, Maximilian, Hagino, Wataru & Shimano, Satoshi, 2019, First record of the family Ameronothridae (Acari: Oribatida) from Japan - new species, juvenile morphology, ecology and biogeographic remarks, International Journal of Acarology 45 (5), pp. 315-327 : 317-326

Genus Ameronothrus Berlese 1896

Type species – Eremaeus lineatus Thorell, 1871

Ameronothrus yoichi sp. n. Pfingstl and Shimano

Zoobank ID. urn:lsid:zoobank.org:pub:5B772E2C-7D5E-4C86-9955-AB84A84C50DA

Type material/locality. Holotype: adult female, Japan, Hokkaido, Yoichi, grey lichen in spray zone on quay wall; 13 September 2018, coll. S.Shimano, T.Pfingstl, S. Hiruta.(Number:TP-20,180,913 – 01). Two paratypes: adult male and adult female; same data as for holotype (numbers: TP-20,180,913 – 02, TP-20,180,913 – 03). All on microscopic slides, deposited at the Collection of Arachnida, Department of Zoology, National Museum of Nature and Science, Tokyo (NMST). Two additional paratypes from the same sample deposited in the collection of the Senckenberg Museum für Naturkunde Görlitz (SMNG) (number: 59,954).

Etymology. The specific name ‘yoichi ” is given as noun in apposition and refers to its type locality.

Differential diagnosis. Colour dark brown, nearly black. Body length 525 – 600 µm. Notogastral cuticle with densely packed large nodules. Prodorsal lamellar keels converging, anteriorly fused by a semicircular translamella. Short clavate sensilli present. Interlamellar and exobothridial setae absent. Labiogenal articulation complete. All adanal setae located posteriorly of anal orifice. Primilateral setae on tarsus I absent. Dorsal companion seta d on genu I, II and III and all tibiae reduced to alveolus. Tarsal distal setae ending with a small nodule.

Description

Measurements. Females (N = 2), length: 563 – 600 μm (mean 582 μm), width: 381 – 388 μm (mean 385 μm); males (N = 3), length: 525 – 556 μm (mean 535 μm), width: 306 – 356 μm (mean 325 μm).

Integument. Cuticle thin, easily deformable, showing large nodules. Cerotegument dense covering conspicuous nodular integument, consequently showing the same pusticulate pattern. Colour dark brown.

Prodorsum. ( Figure 1A , 2A ) Rostrum rounded in dorsal view, demarcated from the remainder of prodorsum by strong transverse caudally arched ridge ct. Obvious lamellar keels (cl) converging, anteriorly fused by semicircular translamella. Transversal ridges originating from bothridia, forming medially several irregular longitudinal ridges next to the anterior border of notogaster. Rostral seta (ro) spiniform, long and smooth (approx. 35 µm). Lamellar seta (le) short, thickened, blunt and smooth (approx. 11 µm). Interlamellar seta (in) and exobothridial seta (ex) absent. Bothridium cup-like, orifice wide and circular. Sensillum (ss) short (approx. 30 µm), strongly clavate, globular head with inconspicuous linear elevations.

Gnathosoma . Palp pentamerous 0 – 2 – 1 – 3-9 (solenidion not included), trochanter very short, femur by far longest segment, genu, tibia and tarsus of almost equal length ( Figure 3A ). Solenidion ω on palptarsus associated with eupathidium acm. Atelebasic rutellum. Distal part with wide paraxial tooth followed by a smaller tooth merging into a series of inconspicuous projections forming an undulating membranous edge ( Figure 3B ). Incision between rutellar teeth with darker sclerotization. Setae a (approx. 15 µm) and m (approx. 25 µm) long, robust and smooth. Mentum regular, seta h setiform, robust (approx. 23 µm). Labiogenal articulation complete. Chelicera chelate, mobile digit darker sclerotized; distinct strong interlocking teeth. Träghårds organ (tg) slender blunt lamella, slightly upward orientated. Seta cha and chb robust and barbed, cha longer (approx. 40 µm), chb shorter (approx. 23 µm) with a strong curvature ( Figure 3C ). No porose areas were detectable on any of the above-mentioned parts.

Gastronotic region. ( Figure 1A , 2A ) Oval in dorsal view, convex in lateral view; no distinct border between anterior median notogastral and prodorsal region. Ascleritic incision pgn visible along the lateral humeral area. Fifteen pairs of short, thickened and blunt notogastral setae (12 – 15 µm), c 1-3, da, dm, dp, la, lm, lp, h 1-3, p 1-3; setae p 1 and h 1 conspicuously longer than others, the latter unilaterally barbed. Five pairs of notogastral lyrifissures present but difficult to trace due to the rough cuticular surface; ia between seta c 2 and c 3, but closer to the latter; im between seta lm and lp; ih laterad and anterior to h 3; lyrifissures ip and ips laterally of seta p 3 and p 2 respectively. Orifice of the opisthonotal gland (gla) not traceable due to heavy ornamention.

Lateral aspect. ( Figure 2C ) Pedotectum I and II absent. Discidium dis between acetabulum III and IV, developed as conspicuous rounded ridge.

Podosoma and venter. ( Figure 1B , 2B ) Epimeral setation 3 – 1 – 2 – 2, all setae setiform and smooth, seta 1b conspicuously longer (approx. 37 µm) than others (10 – 21 µm). Genital orifice large, rectangular with rounded corners, anteriorly broader. Six pairs of genital setae arranged in longitudinal rows, whereas the fifth pair slightly laterally displaced and first slightly longer than others (30 – 37 µm). One pair of short, setiform aggenital setae ag. Strongly curved obvious transversal ridge between genital and anal opening. Anal valves were triangular but strongly rounded. Outer part of preanal organ rectangular with rounded edges, inner part shaped like a transverse bar. Two pairs of thin anal setae, an 1-2 (19 – 25 µm), inserting close to the median border. Three pairs of short adanal setae, ad 1-3 (approx. 12 µm), all located posteriorly of anal orifice. Lyrifissure iad flanking anterior third of anal plates.

Legs. ( Figure 4 ) Ambulacrum tridactylous, median claw broad and strong, lateral claws weaker developed and dorsally slightly dentate. Extensive brachytracheae with slit-like stigmata on the dorsal paraxial face of all femora and tracheal sacculi ventrally on all tibiae and dorsally on trochanter III and IV. Dorsal companion seta d (usually associated with solenidia) on genu I, II and III and all tibiae reduced to alveolus. Primilateral setae of tarsus I absent. Tectal (tc) and iteral (it) setae as well as most other terminal tarsal setae with spoon-shaped or nodular tips (these are sometimes difficult to observe). Famulus ε on tarsus I rod-like, blunt and next to solenidion ω 1, solenidion ω 2 shorter and in slight paraxial position. Solenidia ω 1 and ω 2 on tarsus II adjacent. Chaetome and solenidia see Table 2 .

Common features of juvenile stages

Apheredermous. Colour dark brown. Integument soft and strongly plicate (“ nymphes plissées ” in the sense of Grandjean 1953). Prodorsum triangular, rostrum rounded, cerotegument overall finely granular. Rostral seta (ro) thin, long and smooth, lamellar (le) and interlamellar setae (in) thickened, blunt and barbed. Exobothridial seta (ex) small and smooth. Bothridia small cup-like. Sensillum short, clavate, head with inconspicuous linear elevations. Hysterosoma oval in dorsal view, slightly convex in lateral view. Notogastral setae thickened and barbed, posterior setae longest, median dorsal setae shortest. Orifice of opisthonotal gland gla located in latero-ventral folds on a level with anterior third of anal valves. Legs monodactylous, with slightly invaginated porose areas on the same leg segments and position as brachytracheae in adults. Solenidia on genu and tibia always associated with dorsal companion setae (both approx. the same size).

Larva. (N = 1): length 249 µm.

Prodorsal region. Sensillum short clavate, head drop-shaped with a pointed tip.

Gastronotic region. Twelve pairs of notogastral setae; c 1-3, da, dm, dp, la, lm, lp, h 1-3.

Ventral region of idiosoma. Epimeral setation 2(3)-1-2, third seta on epimeron 1(given in parentheses) developed as protective seta (number in parentheses includes this seta) covering Claparède ’ s organ. No anogenital setae present in this stage. Posterior third of anal orifice framed by smooth and short seta h 3 and smooth and long seta h 2.

Legs. Chaetome and solenidia see Table 2 .

Protonymph. (N = 1): length 286 µm.

Prodorsal region. Sensillum short clavate, head globular without pointed tips from this stage.

Gastronotic region ( Figure 5A , 6A, 6C ). Fifteen pairs of notogastral setae; c 1-3, da, dm, dp, la, lm, lp, h 1-3, p 1-3.

Ventral region of idiosoma ( Figure 5B , 6B ). Epimeral setation 3 – 1-2-1. One pair of short genital setae, placed on the anterior half of genital valves. Adanal and anal setae not developed. Setae p 3 and p 2 short and smooth framing posterior half of anal orifice, seta p 1 longer and slightly barbed.

Legs. Chaetome and solenidia see Table 2 .

Deutonymph. (N = 5): length 356 – 425 µm (mean 388 µm).

Gastronotic region ( Figure 7A, 7C ). Fifteen pairs of notogastral setae, same positions and shapes as in protonymph.

Ventral region of idiosoma ( Figure 7B ). Epimeral setation 3 – 1-2 – 2, seta 4b added in a median position. Two pairs of short genital setae aligned in a longitudinal row. Three pairs of short, smooth adanal setae ad 1-3 flanking anal orifice.

Legs. Chaetome and solenidia see Table 2 .

Tritonymph no data available

Ecological data

The specimen from Muroran ( Figure 8 ) was found in black algae growing on a large rock in the upper eulittoral zone of a rough coastal area with strong wave action. The sea at this location belongs to the North Pacific Ocean but still is influenced by the warm Tsugaru current which flows eastwards through the strait of Tsugaru (between Hokkaido and Honshu connecting the Sea of Japan with the Pacific Ocean). The air temperature at Muroran ranges from −2.6°C in February to 20.1°C in August, while the sea surface temperature ranges from approximately 2°C in the coldest month to 23°C in the warmest month ( Table 3 ).

The population from Yoichi was collected from large layers of grey lichen growing on a massive quay wall. The sample was taken in the supralittoral area, which is only exposed to spray from the seawater. This location lies at the Sea of Japan ( Figure 8 ) in an area that is influenced by the warm northwards flowing Tsushima current. The air temperature in Yoichi ranges from −5°C in the winter to 20°C in the summer, whereas sea surface temperature is the lowest in March with approx. 4.8°C and the highest in September with 23.4°C ( Table 3 ).

Genetic data

COI as well as 18S rRNA sequence data confirm all investigated specimens as members of A. yoichi . The Bayesian inference tree based on 18S rRNA further shows a well-separated A. yoichi clade but places it in paraphyly to Paraquanothrus grahami Norton & Franklin, 2018 , a supposed member of the Ameronothridae ( Figure 9 ). Additionally, A. yoichi as well as P. grahami are placed outside other members of the superfamily Ameronothroidea , namely the Selenoribatidae and Fortuyniidae that are represented as a monophyletic group in the tree.

Discussion

Systematics

The new species Ameronothrus yoichi can be easily distinguished from its congeners by its unique prodorsal ridge structure, the conspicuously pusticulate body surface showing densely packed large nodules and the loss of dorsal companion setae d on all genua in the adult stage. Most other characteristics do overlap with certain species and hence can only be used in specific combinations for determination. Schulte (1975) classified four groups of Ameronothrus species based on morphological correlations, (I) the Ameronothrus marinus group containing A. marinus (Banks, 1896) , A. bilineatus (Michael, 1888) , A. schusteri Schubart, 1970 and A. schubarti Weigmann & Schulte 1975 , (II) the Ameronothrus maculatus group consisting of A. maculatus (Michael, 1882) and A. schneideri (Oudemans, 1903) , (III) the Ameronothrus lineatus group with A. lineatus (Thorell, 1871) and A. nigrofemoratus (L. Koch, 1879) and (IV) the monotypic Ameronothrus lapponicus Dalenius, 1963 group. Ameronothrus yoichi does not share any group-specific characters with the A. marinus and the A. lapponicus group. It shows the same loss of dorsal tibial setae d as the A. lineatus group (whereas in A. yoichi the alveoli of these setae are still present), but differs in all the other group traits. It shares the lack of primilateral tarsal setae (pl), the reduction of the ascleritic incision pgn and the complete labiogenal articulation with the A. maculatus group, but shows a differing anal setation with two instead of only one pair of setae. Based on these conformities, A. yoichi seems to be closest related to A. maculatus and A. schneideri , though there is no complete match with the group. Unfortunately, the descriptions of A. dubinini , A. nidicola and A. oblongus ( Sitnikova 1975, 1977) are not detailed enough for a thorough comparison and classification into one evolutionary group in the sense of Schulte (1975); therefore, a clear assessment of phylogenetic relationships within Ameronothrus is not feasible yet.

The same applies to juvenile morphology as information on the above-mentioned three species and A.nigrofemoratus and A. schubarti is lacking. Nevertheless, a comparison with known Ameronothrus juveniles shows that A. yoichi exhibits the same developmental patterns and formulas, e.g. prodorsal, notogastral, epimeral and anogenital setation, but differs from other species in certain characteristics. Ameronothrus marinus , A. bilineatus and A. schneideri already lack a trichobothrium in the juvenile stages and A. lineatus and A. lapponicus show uniform shapes of notogastral setae (e.g. Schulte 1975; Ermilov et al. 2012) whereas A. yoichi juveniles show enlarged and broadened, posterior notogastral setae. A similar condition can only be found in the nymphs of A. maculatus , but here seta lp is not thickened and enlarged as it is in A. yoichi .

In a larger phylogenetic context, the present data places A. yoichi in a paraphyletic position to Paraquanothrus grahami , the second supposedly member of Ameronothridae . This contrasts with the recently suggested close relationship of Aquanothrinae and Ameronothridae ( Norton and Franklin 2018) and questions its inclusion in the latter. The suggested independent evolutionary origin of the littoral Ameronothridae , Fortuyniidae and Selenoribatidae ( Krause et al. 2016; Pfingstl 2017), all members of the superfamily Ameronothroidea , on the other hand, is supported as shown by their paraphyletic placement. The monophyletic origin of Fortuyniidae and Selenoribatidae was already confirmed by certain studies ( Iseki and Karasawa 2014; Krause et al. 2016) and is further affirmed by the present data. Nonetheless, comprehensive molecular genetic studies, including many more taxa, are necessary to solve found discrepancies and to verify indicated phylogenetic positions.

Ecology

Schulte et al. (1975) postulated four ecological groups of ameronothrid mites: (I) stenotopic inhabitants of the marine littoral populating characteristic salinity ranges, (II) eurytopic inhabitants of the littoral living in saline and brackish waters, (III) holeurytopic inhabitants of the littoral living in limnic, brackish, marine and terrestrial habitats and (IV) stenotopic inhabitants of inland regions dwelling exclusively in terrestrial habitats. Based on the few present records of A. yoichi it is difficult to assess the ecology of this species but the populations were found in the upper marine eulittoral and supralittoral area hence a strictly terrestrial lifestyle (group IV) can be excluded. Additionally, they were found at relatively low latitudes and species occurring at lower latitudes usually tend to be stenotopic and restricted to marine environments ( Schulte et al. 1975; Marshall and Convey 2004); therefore, A. yoichi most likely belongs to group I or II.

Several Ameronothrus species, i.e. A. marinus , A. schusteri , A. lineatus , A. maculatus and A. schubarti are known to live mainly in the sediment-free rock littoral ( Schulte et al. 1975) and the same may apply to A. yoichi as both investigated populations were found restricted to this habitat. Ameronothrus yoichi shows three claws on each leg, which further points to the rocky substrate as the main habitat because tridactylous Ameronothrus species are usually found on solid substrates while monodactylous species are mainly restricted to soft substrates (Schulte 1975).

All Ameronothrus species are microphytophagous and can be classified into three groups, namely, lichenivorous species feeding on lichen growing on hard substrates of the coast or inland, algivorous species subsisting on diverse algae from hard coastal substrates and fungivorous species feeding on fungal growth in the upper soil layer of salt marshes ( Schulte 1976). Based on its predominant occurrence in lichen on a hard substrate, A. yoichi most likely belongs to the lichenivorous group. However, feeding experiments in the lab would be necessary to confirm such a food preference.

In all known cases, Ameronothrus species are larviparous ( Bücking et al. 1998) or at least ovoviviparous ( SØvik 2003), which means that larvae hatch immediately after deposition. The same is true for A. yoichi as we found fully developed larvae contained within eggshells inside the body of a female.

Biogeographic aspects

In a global context, the occurrence of A. yoichi at a latitude of approximately 42 degrees North is nothing unusual because distributions of most Ameronothrus species stretch further south (Schulte 1975). In the Asian Pacific region, on the other hand, represents the record of A. yoichi on Hokkaido the southernmost occurrence of an Ameronothrus species. The congeneric A. nidicola and A. oblongus were reported from Kunashir Island ( Klimov 1998), which is very close to Hokkaido but lies approximately two degrees more northern (see Figure 8 ). The distribution of both species stretches further north with records from Sakhalin Island, Lovushki and the Peninsula of Kamchatka ( Ryabinin 2015). This pattern indicates that A. nidicola and A. oblongus are adapted to colder climates than A. yoichi whereas it is not clear yet how far the distribution of the latter reaches into the South. Records from Honshu are lacking, but occurrences in the north and hence colder regions of this Japanese Island should be considered. Water temperatures seem to play an important role in shaping the distributions of Ameronothrus as shown in A. lineatus and A. nigrofemoratus . These species reach the latitude of 38° North at the Pacific American shore where consistently cold waters prevail while they only reach a latitude of 50° North at European coasts where comparable water temperatures are present (Schulte 1975). Japanese coasts are strongly influenced by the warm Kuroshio Current and its branch the Tsushima Current and these warm waters may limit the southern distribution of Asian Pacific Ameronothrus species.

However, biogeographic studies combined with local climate data would be necessary to prove such a correlation. Unfortunately, most studies on Ameronothrus species provide the locations of the species found, but no detailed information on the climate prevailing at these locations, therefore only vague conclusions might be drawn. In the light of global warming, local climates may change rapidly and hence distributions of Ameronothrus species may alter, therefore providing reference data is the first step in predicting these shifts.

Figures

Figure 1. Ameronothrus yoichi sp. n. adult. (a) male dorsal view, legs omitted; (b) female ventral view, legs distal segments omitted. Scale bar valid for both depictions.

Figure 2. Ameronothrus yoichi sp. n. stereomicroscopic photographs of adult female. (a) dorsal view; (b) ventral view; (c) lateral view.

Figure 3. Ameronothrus yoichi sp. n. mouthparts adult. (a) left pedipalp antiaxial view; (b) left rutellum ventral view; (c) left chelicera antiaxial view.

Figure 4. Ameronothrus yoichi sp. n. adult legs. (a) right leg I antiaxial view; (b) right leg II antiaxial view; (c) left leg III dorsolateral view of antiaxial aspect; (d) left leg IV ventrolateral view of antiaxial aspect.

Figure 5. Ameronothrus yoichi sp. n. protonymph. (a) dorsal view; (b) ventral view. Scale bar valid for both depictions.

Figure 6. Ameronothrus yoichi sp. n. stereomicroscopic photographs of protonymph. (a) dorsal view; (b) ventral view; (c) lateral view. Scale bars 100 µm.

Figure 7. Ameronothrus yoichi sp. n. deutonymph. (a) dorsal view; (b) ventral view, distal leg segments omitted; (c) lateral view, legs drawn simplified.

Figure 8. Distribution of Ameronothrus species in the northern Asian Pacific region.

Figure 9. Bayesian inference tree based on 18S rRNA sequences (1751 bp). Different families are highlighted in colour. Individual sequences of the newly described Ameronothrus yoichi sp. n. species are labelled from JP14_A_03 – JP14_A_11. References and accession numbers for newly created and downloaded sequences can be found in Table 1.