Tricholosporum porphyrophyllum (S. Imai) Guzmán ex T.J. Baroni, Mycologia, 1982

Liu, Yuanchao, Liu, Pu, Hu, Huiping, Li, Dan & Li, Yu, 2016, Tricholosporum, a newly recorded genus of Agaricomycetes in China, Phytotaxa 289 (3), pp. 263-270 : 267-269

Tricholosporum porphyrophyllum (S. Imai) Guzmán ex T.J. Baroni, Mycologia 74(6): 868 (1982) ( FIGURES 1–6 )

≡ Tricholoma porphyrophyllum S. Imai, J. Coll. agric., Hokkaido Imp. Univ. 43: 69 (1938)

≡ Tricholosporum porphyrophyllum (S. Imai) Guzmán, Bol. Soc. Mex. Micol. 9: 63 (1975)

Description:— Pileus 3.5–6 cm, hemispheric, convex to plane, border enrolled slightly when immature, with brown to lilac hues, surface smooth and slightly sticky when wet. Context white, 3–5 mm thick in central. Lamellae adnate or sinuate, crowded, lengths unequal with lamellulae, 3–4 mm broad, violet, slightly changing to brown when bruised. Stipe 3–7 cm long, 0.6–1.0 cm thick at apex, solid, fibrous, violet to grayish violaceous at first, then gradually changing to yellowish to white when mature, cylindrical or slightly expanded downward, white basal mycelium.

Basidiospores starlike or cruciate, 6.25–7×5–5.5 μm, colorless, inamyloid. Basidia clavate, 27.5–30×6.25–7.5 μm, colorless. Pleurocystidia and cheilocystidia absent. Hymenophoral trama parallel, with oleiferous hyphae, 2.8–4.6 μm in diam., with clamp-connections on hyphae of hymenium, and also on hyphae of pileus and stipe.

Specimens examined:— CHINA. Liaoning Province, Fu Shun City, HunHeyuan Nature Reserve of Liaoning Province , N41°52´, E124°50´, 510 m above sea level, 22 August 2011 and 31 July 2012, HMJAU 24949 .

Habitat:—mixed coniferous broad-leaved forest.

Known distribution:— Japan (Hongo, 2001), China.

Comments:— Tricholosporum porphyrophyllum is similar to Tricholosporum subporphyrophyllum in some characteristics, such as the colour of pileus, lamellae and stipe, but slightly different in size. T. subporphyrophyllum is smaller with pileus (2–5.7 cm) and stipe (3–5 cm), and it also has hymenial cystidia that are absent in T. porphyrophyllum ( Guzmán, 1975) . Construction of the phylogenetic tree based on ITS, nlsu, rpb2 sequences showed that the two samples collected in the same nature reserve have high bootstrap support value. The species of T. porphyrophyllum has different taxonomic status in these phylogenetic trees constructed in a different sequence, but there is a higher support that shows it belongs to Tricholomataceae .

Figures

FIGURE 1. The taxonomic position of Tricholosporum porphyrophyllum and other similar genera in the Tricholomataceae based on ITS rDNA phylogeny by using Bayesian algorithm (BA). (The ITS sequence fragment of T. porphyrophyllum was submitted to GenBank (accession No. KU954553, KU954554.))

FIGURE 2. The taxonomic position of Tricholosporum porphyrophyllum and other similar genera in the Tricholomataceae based on nlsu rDNA phylogeny by using Bayesian algorithm (BA). (The nlsu sequence fragment of T. porphyrophyllum was submitted to GenBank (accession No. KU954556, KU954558.))

FIGURE 3. The taxonomic position of Tricholosporum porphyrophyllum and other similar genera in the Tricholomataceae based on rpb2 phylogeny by using Bayesian algorithm (BA). (The rpb2 sequence fragment of T. porphyrophyllum was submitted to GenBank (accession No.KX397356, KX397357))

FIGURE 4. Scanning electron microscope illustrations of basidia and basidiospores of Tricholosporum porphyrophyllum. Photos by Zhang Ailing.

FIGURE 5. Fruitbodies of Tricholosporum porphyrophyllum in the wild. Photos by Liu Yuanchao.

FIGURE 6. Features of Tricholosporum porphyrophyllum (A basidiospores, bar=5μm; B basidia, bar =10μm).