Trimmatostroma salicis Corda, Icon.

Chen, Yanpeng, Liao, Yuechi, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui & Maharachchikumbura, Sajeewa S. N., 2025, Advancements in fungal and fungi-like diversity with a new species and updated records, Phytotaxa 701 (2), pp. 127-147 : 137

Trimmatostroma salicis Corda, Icon. fung. (Prague) 1: 9 (1837) ( FIGURE 5 )

Saprobic on decaying branches of an unidentified woody plant. Sexual morph: Not observed. Asexual morph: Colonies brownish to black, macroscopically visible, caespitose or in erect tufts. Mycelium internal, hyphae not very conspicuous, sparingly branched, pale brown to brown, septate, rather thick-walled, smooth. Stromata lacking. Conidiophores immersed, cylindrical, curved or upright, pale brown, 4–7 μm (x = 5.8 μm) wide, occasionally branched, hyphal filaments gradually developing into fertile threads by becoming somewhat wider and darker. Conidiogenous cells integrated, monoblastic, poorly differentiated, 3–7 × 3–6 μm (x = 5.4 × 4.6 μm, n= 30). Conidia in basipetal chains, multicellular, straight or curved long rod, 2–13-septate, 17–45 × 4–7 μm (x = 28.3 × 5.9 μm, n= 30), pale to dark brown, wall 0.3–0.8 μm (x = 0.6 μm) thick.

Culture characteristics: Conidia germinated on PDA within 12 h at 25 °C in darkness. Colonies white with a dense, uneven texture and a distinct brownish central area, with irregular and slightly raised margins, approximately 12 mm diam. on PDA after 4 weeks at 25°C; on the reverse side, bright orange, dense, and exhibits a radiating, slightly wrinkled texture with smooth and well-defined margins.

Material examined: China, Sichuan Province, Aba Tibetan and Qiang Autonomous Prefecture, Li County, Miyaluo Scenic Resort, 102°48’35” E, 31°42’56” N, elevation 3207 m, on decaying branches of an unidentified woody plant, 19 October 2021, Y.P. Chen and W.H. Tian, MYL57 (HUEST 24.0139), living culture UESTCC 24.0128.

Notes: BLASTn analysis showed that our newly collected isolate UESTCC 24.0128 displayed 99% sequence identity of ITS (661/663, 0 gaps), and 100% sequence identity of LSU to Trimmatostroma salicis CPC 13571. In the multilocus phylogenetic tree ( FIGURE 2 ), isolate UESTCC 24.0128 cluster with T. salicis MFLU 18-0702 formed a strongly supported clade. Morphologically, the newly collected specimen resembles the generic type, Trimmatostroma salicis , in having much more compact and well-delimited sporodochia, up to 13-septate conidia and being non-lichenicolous ( Ellis 1971, Crous et al. 2007). Therefore, we identified the newly collected specimen (HUEST 24.0139) as T. salicis , marking its first recorded occurrence in China.

Figures

FIGURE 2. A phylogram of Trimmatostroma and allied genera was generated from ML analyses based on a combined dataset of ITS and LSU sequence data. Ultrafast bootstrap values from ML and MP>=95 and posterior probability from Bayesian inference>=0.95 are shown above the nodes as ML/MP/BI. The new isolates are in red, and ex-type strains are in bold. The tree was rooted with Variocladium giganteum CBS 508.71.

FIGURE 5. Trimmatostroma salicis (HUEST 24.0139, new country record).A. Natural substrates; B. Sporodochia on natural substrates; C.Close-up of sporodochia; D.Squashed mount of a sporodochium;F–H.Chains of disarticulating conidia; I Growth base of disarticulating conidia; J, K. Colonies on PDA media (front and back views). Scale bar: D = 100 μm, E = 20 μm, F–I = 10 μm.