Aponuphis bilineata ( Baird, 1870 )

Borisova, P. B., Schepetov, D. M. & Budaeva, N. E., 2018, Aponuphis Kucheruk, 1978 (Annelida: Onuphidae) from western African waters, Invertebrate Zoology 15 (1), pp. 19-41 : 29-31

Aponuphis bilineata ( Baird, 1870) species complex

Figs. 1 , 2 , 4 , 5 .

Hyalinoecia bilineata Baird, 1870: 358–359 ; Rioja, 1918: 44; Fauvel, 1936: 51; Bellan, 1960: 18; Intes, Le Loeuff, 1975: 309.

Hyalinoecia rubra Langerhans, 1880: 292 .

Aponuphis bilineata . — Kucheruk, 1978: 91; Aguirrezabalaga et al., 2002: 27; Arias, Paxton, 2015: 358-360 .

MATERIAL EXAMINED:

WesternAfrica, RV FridtjofNansen: ZMBN 91344, Morocco, St. GR37 (1 DNA voucher); ZMBN 91345, Morocco, St. GR37 (1 DNA voucher); ZMBN 91346, Morocco, St. GR40 (1 DNA voucher); ZMBN 91348, Morocco, St. GR49 (1 DNA voucher); ZMBN 111713, West Sahara, St. GR28 (1 DNA voucher); ZMBN 111714, Morocco, St. GR37 (1 DNA voucher); ZMBN 111719, Morocco, St. GR40 (1 DNA voucher); ZMBN 91413, Morocco, St. GR37 (1 DNA voucher); ZMBN 91348, Morocco, St. GR-49 (1); ZMBN 91344, West Sahara, St. GR-28 (8); ZMBN 91347, Morocco, St. GR40 (6); ZMBN 91341, Morocco, St. SL38 (5, 1 on SEM stub); ZMBN 91342, Guinea, St. 7GU-3C (1); ZMBN 91415, Morocco, St. GR37 (1 on SEM stub); ZMBN 91340, São Tomé and Príncipe, St. E5-7-5B (10).

Mediterranean : ZMBN 111716 , Spain, off Mallorca, 39.72125°N 3.46395E °, 10–18 m (1 DNA voucher); ZMBN 111717 , Spain, off Mallorca, 39.72125°N 3.46395E °, 10–18 m (1 DNA voucher).

North Sea : ZMBN 91718 , Norway, St. SFB-11_11, 61.213°N 1.816°E, 148 m (1 DNA voucher); ZMBN 91720 , Norway, St. SFNE-01_11, 61.432°N 1.927°E, 266 m (1 DNA voucher).

TYPE LOCALITY: off Cornwall, Great Britain, 73 m.

REMARKS: Antennae reach chaetiger 10– 15 ( Fig. 4A ). First 6–7 chaetigers with tridentate falcigers ( Fig. 4E, F ), bidentate falcigers were reported in previous descriptions ( Arias, Paxton, 2015) but were absent in the studied material. Subulate ventral cirri present on the first five chaetigers ( Fig. 4B, C ). Branchiae simple strap-like, starting from chaetiger 5. Subacicular hooks from chaetiger 10 ( Fig. 4D ). Three clades are recovered within A. bilineata ( Fig. 3A–C ). Two clades (A and C) have the typical color pattern of two large spots on the peristomium continuing into two longitudinal orange stripes on the dorsal side. Additional lateral pigmented spots are visible on each segment at the bases of the parapodia ( Figs 1A , 2A ). This pattern was named by Arias & Paxton (2015) as morph (1). Morph (2) was described as “peristomium and first 3–4 chaetigers with two large brownish spots almost filling dorsal surface of segment, sometimes coalescing medially; from chaetiger 4–5 to median region two longitudinal dorsal pigmented stripes discoursing laterally close to parapodia” ( Arias, Paxton, 2015). This morph was not present in the studied material.

Clade C comprised specimens collected off Mallorca, Spain with slightly pigmented peristomium and a pair of lateral pigmented dots on each segment ( Figs 1B , 2B ) representing the third previously unknown color morph in this species complex.

DISTRIBUTION: East Atlantic, North Sea, Mediterranean, in western Africa from Morocco to São Tomé and Príncipe. Clade A — North Sea, off Morocco and Western Sahara, 101–266 m; clade B — off Morocco, 102 m; clade C — off Mallorca, Spain, 10–18 m ( Fig. 5 ).

Figures

Fig. 1. Various color morphs in Aponuphis. A, B — A. bilineata; C — A. ornata; D–F — A. brementi. Рис. 1. Цветовые морфы в роде Aponuphis. A, B — A. bilineata; C — A. ornata; D–F — A. brementi.

Fig. 2. Schematic interpretation of various color morphs in Aponuphis. A, B — A. bilineata; C — A. ornata; D–F — A. brementi. Рис. 2. Схематическое иЗображение цветовых морф в роде Aponuphis. A, B — A. bilineata; C — A. ornata; D–F — A. brementi.

Fig. 4. Morphology of Aponuphis bilineata. A — anterior part of the body, lateral view; B — parapodia of chaetigers 3–5, arrow indicates the first branchia on parapodium 5; C — parapodia of chaetigers 5–7, arrow indicates ventral glandular pad on parapodium 6; D — parapodia of chaetigers 9–11, arrow indicates subacicular hooks on parapodium 10; E, F — pseudocompound falcigers from anterior parapodia. A–E — scanning electron micrographs; F — light micrograph. Рис. 4. Морфологические приЗнаки Aponuphis bilineata. A — передний отдел тела, вид сбоку; B — параподии 3–5-го сегментов, стрелка укаЗывает на первую жабру на 5-м сегменте; С — параподии 5– 7-го сегментов, стрелка укаЗывает на вентральную желеЗистую подуШечку на 6-м сегменте; D — параподии 9–11-го сегментов, стрелка укаЗывает на субацикулЯрные крючьЯ на 10-м сегменте; E, F — псевдосочленные крючковидные Щетинки на передних параподиЯх. A–E — сканируюЩие Электрон- ные микрофотографии; F — световаЯ микрофотографиЯ.

Fig. 5. Distribution of Aponuphis bilineata. Black star indicates the type locality; black circles indicate localities from which genetic data were available; white circles indicate localities without genetic data; white squares indicate data from literature (Arias, Paxton, 2015). Рис. 5. Распространение Aponuphis bilineata.Чер- ной ЗвеЗдой отмечено типовое местонахожде- ние; черными кругами отмечены станции, длЯ которых получены молекулЯрно-генетические данные; белыми кругами отмечены станции, длЯ которых молекулЯрно-генетических данных нет; белыми квадратами отмечены данные иЗ литера- туры (Arias, Paxton, 2015).

Fig. 3. Phylogenetic tree resulting from the Neighbor Joining analysis of 16S rDNA sequences of Aponuphis species. Numbers on nodes indicate bootstrap support. Capital letters correspond with the clades discussed in the text. Рис. 3. Филогенетическое дерево, построенное методом ближайШего соседа на основе последова- тельностей гена 16S рДНК длЯ видов Aponuphis. ЗначениЯ в уЗлах дерева отображают поддержку бутстреп. Заглавные буквы соответствуют кладам, обсуждаемым в тексте.