Neopyrenochaetopsis machili Y.Z. He, 2025

He, Yong-Ze, 2025, Neopyrenochaetopsis machili sp. nov. on Machilus nanmu from Shanxi Province, China, Phytotaxa 696 (3), pp. 224-234 : 229-230

Neopyrenochaetopsis machili Y.Z. He , sp. nov.

MycoBank: 856714

Fig. 2

Etymology. The specific epithet refers to the genus of the host plant Machilus nanmu .

Type. China, Shanxi Province: Taiyuan City, Longcheng Forest Park, on diseased leaves of Machilus nanmu (Oliv.) Hemsl. (1200m; 37.8°N, 112.5°E), 15 Oct. 2023, Y. Z. He, holotype HSAUP1175 , ex-type CGMCC3.28266 .

Description. Colonies on PDA incubated at 25 °C in the dark, reaching 73–76 mm diam., margin scalloped or irregularly round, center olive green and flavogreen edges, aerial mycelium moderately developed, reverse similarity. Conidiomata solitary or aggregated, formed on agar surface, black, globose to subglobose, 150–250 × 150–300 mm. Conidiophores are inapparent and often reduced to conidiogenous cells. Conidiogenous cells phialidic, ampulliform, straight or slightly curved, slightly branched, 4.2–7.7 × 2–2.6. Conidia solitary, obovoid to ellipsoid, 2.8–3.8 × 1.5–2.2 μm, mean ± SD = 3.3 ± 0.2 × 1.8 ± 0.2 μm, hyaline, 1–2 guttulate, smooth, apex obtuse, base with inconspicuous hilum. Sexual morph not observed.

Additional specimens examined: China, Shanxi Province: Taiyuan City, on leaves of Machilus nanmu (Oliv.) Hemsl. , 15 Oct. 2023, Y.Z. He, HSAUP1125, living culture H1125.

Notes. Phylogenetic analyses showed that Neopyrenochaetopsis machili formed an independent clade closely related to Neopyrenochaetopsis hominis CBS 143033 ( Fig. 1 ). Neopyrenochaetopsis machili was distinguished from N. hominis (CBS 143033) by 5/815 base pairs in the LSU, 47/ 537 in the ITS, 50/ 304 in the tub, and 153/ 874 in the rpb2, including gaps. The conidia of N. machili were similar to those of N. hominis (2.8–3.8 × 1.5–2.2 vs. 3–3.5 × 1.5–2 µm), but the conidiomata and colonies of N. machili differed from those of N. hominis (black vs. brown). The new isolates were described here as a new species based on their distinct morphological characteristics and DNA based differences as recommended by Jeewon and Hyde (2016).

Figures

FIGURE 1. Phylogram of Pyrenochaetopsidaceae based on combined LSU, ITS, tub and rpb2 genes. The BI and ML bootstrap support values above 0.70 BYPP and 70 % are shown at the first and second position above nodes. Strains from the current study are in red. Some branches were shortened to fit them to the page – these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines.

FIGURE 2. Neopyrenochaetopsis machili (CGMCC3.282660). a Leaves of host plant. b Surface of colony after 15 days on PDA. c Reverse of colony after 15 days on PDA. d Conidiomata sporulating on PDA. e–f Conidiogenous cells with conidia. g–h Conidia. Scale bars = 10 μm (e–h).