Vaginicola tincta, EHRENBERG, 1830

Lu, Borong, Li, Lifang, Hu, Xiaozhong, Ji, Daode, Al-Rasheid, Khaled A S & Song, Weibo, 2019, Novel contributions to the peritrich family Vaginicolidae (Protista: Ciliophora), with morphological and phylogenetic analyses of poorly known species of Pyxicola, Cothurnia and Vaginicola, Zoological Journal of the Linnean Society 187 (1), pp. 1-30 : 14

VAGINICOLA TINCTA EHRENBERG, 1830

( FIGS 6A–I , 7A–U; TABLES 1, 6, 7)

Vaginicola tincta was originally described by Ehrenberg (1830) and was subsequently redescribed several times ( Ehrenberg 1838; Kahl, 1935; Sommer, 1951; Stiller, 1971; Vucetich & Escalante, 1979; Foissner et al., 1992; Kreutz & Foissner, 2006; Shen & Gu, 2016). However, its ciliature is still unknown. We here provide an improved diagnosis based on present and previous observations.

Figures

Figure 6. Vaginicola tincta in vivo (A–G) and after protargol staining (H, I). A, B, two individuals with single zooid (A) and two zooids (B). C, D, two contracted individuals. E, striations of pellicle. F, apical view of zooid, showing the location of contractile vacuole. G, macronucleus in vivo. H, showing macronucleus and the trochal band. I, oral ciliature. Abbreviations: CV, contractile vacuole; EM1–2, epistomial membrane 1–2; G, germinal kinety; H, haplokinety; In, infundibulum; Ma, macronucleus; Po, polykinety; P1–3, infundibular polykineties 1–3; TB, trochal band. Scale bars = 80 μm (A–G), 40 μm (H).

Figure 7. Photomicrographs of Vaginicola tincta in vivo (A–N) and after protargol staining (O–U). A–F, lateral views of different individuals with single zooid (A, C–E) and two zooids (B, F), showing varieties of body shape, lorica shape and colour. G, H, two contracted individuals. I, arrow indicates the contractile vacuole. J, apical view of zooid; arrow indicates the contractile vacuole, arrowhead indicates the infundibular. K, apical view of lorica. L, anterior portion of lorica; arrow indicates the everted aperture. M, posterior portion of lorica; arrow indicates the fluctuation of lorica. N, striations of pellicle; arrow indicates the trochal band. O–Q, three stained specimens; arrows indicate the trochal band. R, T, oral ciliature; asterisks indicate the beginning of haplokinety and polykinety. S, U, infundibular polykineties 1–3; asterisks indicate the ending of row1 within P2 and P3. Abbreviations: EM1–2, epistomial membrane 1–2; G, germinal kinety; H, haplokinety; Ma, macronucleus; Po, polykinety; P1–3, infundibular polykineties 1–3. Scale bars = 80 μm (A–H), 40 μm (O–Q).