identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03CC87BEF401C22397DEF854C330AA62.text	03CC87BEF401C22397DEF854C330AA62.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification and sequencing</p><p>DNA was extracted from 5-10 mg tissue of dried specimen with the Broad-spectrum Plant Rapid Genomic DNA Kit (Biomed, Beijing, China) following the manufacturer’s instructions and preserved at −20℃.</p><p>This study used primers ITS4 and ITS5 (White et al. 1990) for the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA repeat, primers LROR and LR5 (Moncalvo et al. 2000) for the Large Subunit (nrLSU). Genes were amplified by polymerase chain reaction (PCR) using the procedures mentioned in (Li et al. 2022; Wang et al. 2024b; Yang et al. 2024a; Han et al. 2024; Li et al. 2024b). The PCR products were sent to a commercial biotech company (Baimaide Biotechnique Company, Beijing, China) for sequencing region.</p></div>	https://treatment.plazi.org/id/03CC87BEF401C22397DEF854C330AA62	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Yang, Wen-Qiang;Zhang, Jia-Rui;Cao, Man-Ting;Hu, Zuo-Lu;Zhao, Rui-Lin	Yang, Wen-Qiang, Zhang, Jia-Rui, Cao, Man-Ting, Hu, Zuo-Lu, Zhao, Rui-Lin (2025): Two new species of Collybiopsis (Agaricales, Omphalotaceae) from Beijing, China. Phytotaxa 715 (1): 47-60, DOI: 10.11646/phytotaxa.715.1.3, URL: https://doi.org/10.11646/phytotaxa.715.1.3
03CC87BEF407C22997DEFF22C6C8AEE2.text	03CC87BEF407C22997DEFF22C6C8AEE2.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Collybiopsis beijingensis R. L. Zhao & W. Q. Yang 2025	<div><p>Collybiopsis beijingensis R.L. Zhao &amp; W.Q. Yang, sp. nov. Figure 2.</p><p>Fungal name: FN 572124</p><p>Diagnosis: The prominent features of this species include a greyish orange to brownish and medium-sized pileus; medium-sized basidiospores (6.4–8.9 × 3.3–3.9 µm) with ornamentation less developed; scattered to gregarious on rotting pine needles.</p><p>Holotype: China, Beijing, Miyun District, Bulaotun Town, Yunfeng Mountain, 28 August 2023, Bin Cao, Minyu Zhu and Bei Han, ZRL20234937 (HMAS 282425, GenBank accession-ITS: PQ408926, LSU: PQ40895).</p><p>Etymology: ‘ beijing ’ refers to the location (Beijing)where the type specimen was collected.</p><p>Macroscopic description:— Pileus 14‒25 mm wide, shallowly depression to plane by maturity, wavy edges, surface smooth, dry, greyish orange (6B3) to brownish (7D8 to E8). Lamellae subdistant, L= 6–9, I= 1–3, almost free to adnate, in two major ranks with no anastomosis, whitish to yellowish white (3A2). Stipe 48–56 × 4‒5 mm, central to eccentric, cylindrical, when fresh reported as concolorous with pileus, apex yellowish white (3A2) to light brown (6D4), edge even. Context thin, white (3A2). Odor and taste not recorded.</p><p>Microscopic description:— Basidiospores 6.4–8.9(9.2) × (3.1) 3.3–3.9(4.5) µm, Q= 1.9–2.3, avQ = 2.1, broadly ellipsoid, amygdaliform, smooth, thin-walled, hyaline, non-dextrinoid, with drops. Basidia 23.6–26.5(32.6) × 4.1– 6.2(6.7) µm, 2 or 4 sterigmata, sterigmata 2.1–3.5 µm long, clavate, often constricted, sterigmata robust, slightly curved, oil droplets present. Cheilocystidia (25.2)30.1–36.7(37.7) × 4.5–6.3 (9.3) µm, broadly clavate, irregular, sometimes lobed, hyaline, thin-walled. Pleurocystidia (21.9)25.5–33.5 × (3.8)4.2–6.6 µm, abundant, clavate, subulate, sometimes lobed, contents homogeneous to heterogeneous with crystal-like inclusions, hyaline, firm-to thick-walled. Caulocystidia absent. Trama hyphae cylindrical, often sub-inflated, smooth, non-dextrinoid 1.7–5.8 µm wide. Pileipellis cylindrical, smooth, sometimes swollen, thin-walled, 2.2‒7.9 µm wide hyphae; Stipitipellis cylindrical, parallel along stipe, smooth, sometimes swollen, thin-walled, 2.4‒4.5 µm wide hyphae. Clamp connections present in all tissues.</p><p>Habitat and distribution: Scattered to gregarious on rotting pine needles in coniferous forest from summer to autumn.</p><p>Additional specimens examined: China, Beijing, Miyun District, Xinchengzi Town, Yunxiu Valley, 15 August 2023, Bin Cao, Min-yu Zhu and Bei Han, ZRL20232835 ; China, Beijing, Miyun District, Bulaotun Town, Yun feng Mountain, 28 August 2023, Bin Cao, Min-yu Zhu and Bei Han, ZRL20234938 ; China, Beijing, Mentougou District, Pearl Lake Scenic Area, 5 September 2023, Jia-xin Li, Wen-qiang Yang and Ze-zhi Wang, ZRL20232473 ; China, Beijing, Yanqing District, Luo jia tai Village, 18 August 2023, Jia-xin Li, Wen-qiang Yang and Ze-zhi Wang, ZRL20233443 .</p><p>Notes: Collybiopsis beijingensis can be easily distinguished by its greyish orange to brownish pileus. Microscopically, this new species has medium-sized spores with less developed ornamentation. In the field, this new species is morphologically similar to C. confluens (Hughes &amp; Petersen 2015), which is known as a representative example of intra-specific variation between continents (Kim et al. 2022). Collybiopsis confluens is distinguishable from C. beijingensis by its denser lamellae and larger cheilocystidia (34–77 × 2.5–4 µm) (Hughes &amp; Petersen 2015), which the two species are phylogenetically distant from each other. Collybiopsis ramealis is another morphological similar species to this new species. However, this known species has a smaller basidiocarp (2–20 mm), bigger basidiospores (7.8–11 × 2.5–4 µm) and different type of pileipellis (Noordeloos 1983; Desjardin et al. 1997; Kim et al. 2022). In this study, phylogenetic analysis demonstrated that five specimens of this new species formed a cohesive cluster with full support (Fig 1). Additionally, they were sister to C. peronata (Fig 1). Collybiopsis peronata fewer and buff lamellulae (1–3), a thicker stipe (3–8 mm), longer basidia (20–40 µm), and longer cheilocystidia (25–90 × 5–10 µm) (Noordeloos et al. 1999; Kim et al. 2022).</p></div>	https://treatment.plazi.org/id/03CC87BEF407C22997DEFF22C6C8AEE2	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Yang, Wen-Qiang;Zhang, Jia-Rui;Cao, Man-Ting;Hu, Zuo-Lu;Zhao, Rui-Lin	Yang, Wen-Qiang, Zhang, Jia-Rui, Cao, Man-Ting, Hu, Zuo-Lu, Zhao, Rui-Lin (2025): Two new species of Collybiopsis (Agaricales, Omphalotaceae) from Beijing, China. Phytotaxa 715 (1): 47-60, DOI: 10.11646/phytotaxa.715.1.3, URL: https://doi.org/10.11646/phytotaxa.715.1.3
03CC87BEF408C22B97DEFA04C224A992.text	03CC87BEF408C22B97DEFA04C224A992.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Collybiopsis lignicola R. L. Zhao & W. Q. Yang 2025	<div><p>Collybiopsis lignicola R.L. Zhao &amp; W.Q. Yang, sp. nov. Figure 3.</p><p>Fungal name: FN 572125</p><p>Diagnosis: The distinctive features include a deep brownish and whitish radially fibrillose pileus, scattered or gregarious on the bark of deciduous trees, broadly ellipsoid to fusiform basidiospores.</p><p>Holotype: China, Bejing, Changping District, Dalinggou Village, Kiwi Fruit Valley Scenic Spot , 11 August 2023, Jia-xin Li, Wen-qiang Yang and Ze-zhi Wang, ZRL20233191 (HMAS 282429, GenBank accession-ITS: PQ408934, LSU: PQ408957).</p><p>Etymology: ‘ lignicola ’ refers to growing from rotten wood.</p><p>Macroscopic description:— Pileus 12–24 mm broad, convex to hemispherical when young, becoming planoconvex to flat with an uplifted margin with age, when fresh rich deep brown (6E6-7), surface smooth, radially fibrillose, white (3A2). Lamellae distant, L= 5–11, I = 0.7–1, free, whiteish to yellowish white (3A2). Context thin, white (3A2). Stipe 17–24 × 1.5‒1.9 mm, central to eccentric, cylindrical, tomentose, apex white to yellowish white (3A2), gradually becoming deep downwards (7D2 to 7E6), covered with pubescence. Odor and taste not recorded.</p><p>Microscopic description:— Basidiospores 5.1–7.4 × 3.1–4.3 µm, Q = 1.65–1.72, avQ = 1.69, broadly ellipsoid to fusiform, smooth, hyaline, non-dextrinoid, with drops, germ pore absent. Basidia (18.4)20.4–33.6 × (2.1)4.6–9.0 µm, 4-spored, narrowly clavate, colorless, with oil droplets or pigment, sterigmata 1.5–3.5 µm long. Cheilocystidia (18.5)20.1–29.0 × (3.7)4.2–6.5(7.1) µm, utriform to narrowly lageniform, some cylindrical to narrowly clavate, hyaline, with obtuse or sub-capitate apex, base pedunculate or pedicellate, non-gut, well-defined basal clamp. Pleurocystidia 25.5‒28.6 × 4.6‒6.7 µm, clavate, fusiform, hyaline, with obtuse or sub-capitate apex. Caulocystidia absent. Trama hyphae cylindrical, nearly inflated, branched, smooth, non-dextrinoid, 2.4‒5.1 µm wide. Pileipellis cylindrical, smooth, sometimes swollen, thin-walled, 3.1‒5 µm wide hyphae; Stipitipellis cylindrical, parallel along stipe, smooth, sometimes swollen, thin-walled, 1.9‒5 µm wide hyphae. Clamp connections present in all tissues.</p><p>Habitat and distribution: Scattered or gregarious on the bark of deciduous trees in summer and autumn.</p><p>Additional specimens examined: China, Bejing, Miyun District, Dongshaoqu Township, 9 August 2023, Bin Cao, Min-yu Zhu and Bei Han, ZRL20232668 ; China, Bejing, Changping District, Xixincheng Village, Taoyukou Reservoir, 10 August 2023, Jia-xin Li, Wen-qiang Yang and Ze-zhi Wang, ZRL20233136 .</p><p>Notes: Collybiopsis lignicola can be easily distinguished by its deep brownish and whitish radially fibrillose pileus. In the field, C. lignicola appears similar to C. biformis and C. menehune . Collybiopsis biformis differs from this new species by its elongated basidiospores (6.4‒9.2 × 2.4‒4.8 µm) and smaller basidia (&lt;22 µm long) (Kim et al. 2022). Collybiopsis menehune can be distinguished from C. lignicola by its larger pileus (8‒30 mm) and longer basidiospores (7.5‒9.5 × 3.5‒4.2 µm) (Desjardin et al. 1999; Kim et al. 2022). In the phylogenetic tree (Fig 1), C. dichrous and C. dichroa are sisters to C. lignicola . Morphologically, they can be easily distinguished and C. dichroa has shorter stipe (&lt;2 cm long) and has reddish-brown color of pileus surface (Kim et al. 2022). Collybiopsis dichrous has larger basidiospores (8.8–12.8 × 4.0–5.6 µm) and larger basida (29–40 × 6–8 µm) (Mata et al. 2002).</p></div>	https://treatment.plazi.org/id/03CC87BEF408C22B97DEFA04C224A992	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Yang, Wen-Qiang;Zhang, Jia-Rui;Cao, Man-Ting;Hu, Zuo-Lu;Zhao, Rui-Lin	Yang, Wen-Qiang, Zhang, Jia-Rui, Cao, Man-Ting, Hu, Zuo-Lu, Zhao, Rui-Lin (2025): Two new species of Collybiopsis (Agaricales, Omphalotaceae) from Beijing, China. Phytotaxa 715 (1): 47-60, DOI: 10.11646/phytotaxa.715.1.3, URL: https://doi.org/10.11646/phytotaxa.715.1.3
