taxonID	type	description	language	source
03DF87D1FFD6FF87FF0CFBB4DB156C9D.taxon	materials_examined	Type material. Holotype: male, cephalothorax length 0.4 mm, total length 4.7 mm, Brazil, Minas Gerais state, Presidente Olegário municipality, Vereda da Palha Cave (18 º 15 ' 17 " S 46 ° 07 ' 32 " W), MZUSP 28420, 13. X. 2010, Ferreira, R. L. & Cols. Paratypes: UFRGS 5542 (1 male on slide, 1 male, 1 female), MZUSP 28421 (1 male, 1 female), UFLA 0 261 (1 male on slide, 2 males, 2 females), with the same data as holotype.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFD6FF87FF0CFBB4DB156C9D.taxon	diagnosis	Diagnosis. Eyes reduced or absent in some specimens. Antenna 1 and antenna 2 of similar size. Gnathopod 1 and 2 carpus posterior lobe with polygonal pattern and two rows of denticles as comb scales. Gnathopod 1 propodus inner face with five serrate setae, posterior margin with denticles as comb scales; dactylus with denticles as comb scales. Gnathopod 2 propodus hammer-shaped, posterior margin with denticles as comb scales, irregular palm, longer than posterior margin. Uropod 1 inner ramus of male with one curved seta and five apical cuspidate setae with accessory seta. Uropod 3 peduncle with four cuspidate setae with accessory seta, ramus with three to four cuspidate setae.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFD6FF87FF0CFBB4DB156C9D.taxon	description	Description of male. (Figs 1 D and 3 A). Mean body length: 4.3 ± 0.9 mm (n = 15); mean cephalothorax length: 0.4 ± 0.09 mm. Body surface smooth, epimeral plates not acuminate. Round and pigmented eyes, with few ommatidia or eyeless (n = 45; 4.4 % of the sample). Antenna 1 (Fig. 3 B) total length reaches fifth pereonite; peduncle length exceeds first pereonite; flagellum with 9 to 11 articles, with two aesthetascs on each article after article 5. Antenna 2 (Fig. 3 C) total length reaches sixth pereonite; peduncle surpasses the first pereonite; flagellum with 9 to 11 articles. Upper lip (Fig. 3 D) margin rounded, distal border covered by setules on dorsal and ventral faces. Mandible (Fig. 3 E) basic amphipodan (in the sense of Watling 1993), but without palp; incisor toothed; left lacinia mobilis with five teeth and setal row with three pappose setae; right mandible with three pappose setae; molar process broad and cylindrical with accessory seta. Lower lip (Fig. 3 F) lobes rounded, with setules on dorsal and ventral faces. Maxilla 1 (Fig. 3 G) inner plate shorter than outer plate, with two distal papposerrate setae, and several marginal setules. Outer plate with eight to nine serrate setae. Palp short, uniarticulated with few distal setules. Maxilla 2 (Fig. 3 H) inner and outer plates of similar sizes, inner plate with two papposerrate setae, five serrulate setae and several simple setae; outer plate with several simple distal setae. Maxilliped (Fig. 3 I) inner plate with three cuspidate distal setae and several pappose and simple setae; outer plate with simple setae on the margin; palp with four articles with several simple setae. Dactylus unguiform, smaller than third article, with simple seta and distal nail. Gnathopod 1 (Fig. 4 A) subchelate; basis, ischium and merus with simple setae; carpus longer than wider, longer than propodus, lobe posterior margin with polygonal pattern, two rows of denticles as comb scales and a row of serrate setae; propodus length 1.5 times the width (rectangular), hammer-shaped, inner face with five serrate setae, posterior margin with denticles as comb scales; dactylus claw-like with simple setae, one plumose seta and denticles as comb scales (Fig. 4 B). Gnathopod 2 (Fig. 4 C) subchelate; basis, ischium and merus with simple setae on the posterior margin; carpus wider than longer, lobe posterior margin elongated with polygonal pattern, two rows of denticles as comb scales and a row of serrate setae; propodus longer than wider (rectangular), posterior margin with denticles as comb scales, irregular palm, slope oblique strongly inclined, palm 1.1 times longer than posterior margin, margin convex with several simple setae and cuspidate setae with accessory seta (15 - 18); dactylus claw-like with a plumose seta (Fig. 4 D). Pereopods 3 to 7 (Figs. 4 E and 4 F) merus, carpus, propodus posterior margin with cluster of cuspidate setae with accessory seta; dactylus length 1 / 3 of propodus; propodus dorsal and distal margins with simple setae; pereopod 3 and 4 with similar sizes; pereopod 5 smaller than others; pereopod 6 smaller than pereopod 7, which is about 1.2 times longer than pereopod 3. Pleopods (Fig. 5 A) peduncle shorter than rami, with distal coupling spines; both rami with several plumose setae. Uropod 1 (Fig. 5 B) peduncle shorter than rami, with five cuspidate setae with accessory seta; inner ramus longer than outer ramus with two cuspidate setae on inner margin, one curved seta and five apical cuspidate setae (Fig. 5 C); outer ramus with three cuspidate setae with accessory seta on inner margin, and apex with three cuspidate setae and a simple seta (U 1 about 5 times the length of U 3). Uropod 2 (Fig. 5 D) shorter than uropod 1; peduncle shorter than rami, with three cuspidate setae on the margin; inner ramus longer than outer ramus, margin with cuspidate seta with accessory seta, apex with three cuspidate setae and two cuspidate setae with accessory seta; outer ramus with a cuspidate seta with accessory seta on the margin, apex with four cuspidate setae, one with accessory seta. Uropod 3 (Fig. 5 E) shorter than uropod 2; peduncle longer than wider with four distal cuspidate setae with accessory seta; ramus uni-articulated, as long as peduncle, with three to four distal cuspidate setae. Telson (Fig. 5 F) as wide as long, with two apical cuspidate setae and three plumose setae laterally. Coxal gills sac-like present on pereonites 2 to 6. Sternal gills tubular present on pereonites 2 to 7. Female. Mean cephalothorax length: 0.4 ± 0.05 mm, mean body length: 4.5 ± 0.9 mm (n = 3). Gnathopod 1 (Fig. 6 A) shorter than gnathopod 2, but wider; carpus longer than wider, lobe posterior margin with polygonal pattern and denticles as comb scales on the margin, propodus longer than wider, inner face with four serrate setae. Gnathopod 2 (Fig. 6 B) carpus longer than wider, lobe posterior margin with polygonal pattern and denticles as comb scales; propodus rectangular, longer than wider (2 times), inner face with three serrate setae. Uropod 1 (Fig. 6 C) peduncle longer than rami, with four cuspidate setae; inner ramus with two cuspidate setae on the margin, apex with five cuspidate setae, three of them with accessory seta; outer ramus with three cuspidate setae on the margin and four distal setae, one of them with accessory seta.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFD6FF87FF0CFBB4DB156C9D.taxon	etymology	Etymology. The specific epithet veredae refers to the location where the species was found. Habitat and ecological considerations. The species was found in a freshwater underground stream, in Vereda da Palha Cave, located in the municipal district of Presidente Olegário, near Varjão de Minas city (Fig. 1 A). The cave is situated in the karst area belonging to the Bambui group, which is extended through the states of Minas Gerais, Goiás, Tocantins and Bahia. The known cave extension corresponds to 1,300 meters, with at least three levels. The lower level is characterized by the presence of a drainage formed by a stream that descends in one of the cave entrances (Fig. 1 B). No individual was observed in this drainage. Specimens were only found at the second level, which has a succession of travertines (Fig. 1 C) filled by percolating water probably from epikarst compartments, especially in dry seasons. The main resource observed in these pools consisted of organic matter. In the first sampling (13. X. 2010), dozens of individuals were observed swimming on small branches, in a very deep puddle. The water was crystal clear, allowing to observe the bottom and of the specimens. On the same visit, several couples were found in pre-copulatory behavior. On a subsequent visit to the cave (31. I. 2011), only one specimen was found. On the occasion, the water from the travertines was quite turbid, indicating that the habitat changes over the year, not only receiving waters from the epikarst compartments. The absence of individuals in this occasion might indicate that the organisms are capable of migrating to other underground compartments, thus avoiding turbid waters.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFD6FF87FF0CFBB4DB156C9D.taxon	discussion	Remarks. Hyalella veredae represents the first troglobitic species described for the state of Minas Gerais, south-eastern Brazil. H. veredae resembles H. spelaea due to the presence of reduced eyes, while in H. caeca, H. imbya, H. anophthalma and H. muerta the eyes are absent. However, a small part of the population (4.4 %) did not present eyes. Culver et al. (1995) described a similar process in cave populations of Gammarus minus Say, 1818 that had different degrees of eyes reduction and different sizes of the antennae. These authors suggest that intraspecific variation may be consequence of time and degree of isolation of each population in underground environments. In addition to the eyes, H. veredae shows different antenna sizes, in which antenna 1 and 2 are subequal in length, The shape of male gnathopod 2 of H. veredae is similar to H. spelaea, H. anophthalma and H. muerta, showing irregular palm, differing from H. caeca and H. imbya, both having a smooth palm. The palm inclination of male gnathopod 2 of H. veredae is similar to H. imbya, both showing palm strongly inclined. The presence of one curved seta in uropod 1 inner ramus is similar to H. imbya. Moreover, it was observed that the number of serrate setae on propodus gnathopod and on uropod 3 is different in H. veredae in comparison to other troglobitic species.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFDEFF80FF0CFE02DB396F9D.taxon	materials_examined	Type material. Holotype: male, cephalothorax length 0.4 mm, total length 5.6 mm, Brazil, Paraná state, Ponta Grossa municipality, Andorinhas Cave (25 ° 08 ’ 39 ” S 49 ° 55 ’ 58 ” W), MZUSP 28419; 24. VII. 2011, Ferreira R. L. & cols. Paratypes: UFRGS 5543 (2 males on slides, 1 female on slide), UFLA 0 260 (1 male, 1 female). All samples have the same data as holotype.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFDEFF80FF0CFE02DB396F9D.taxon	diagnosis	Diagnosis. Eyes absent. Antenna 1 flagellum with 11 to 13 articles, longer than antenna 2. Antenna 2 with eight to ten articles. Gnathopod 1 and 2 carpus posterior lobe with polygonal pattern and one row of serrate setae. Gnathopod 1 propodus oval shape, without projections on the posterior margin, inner face with five serrate setae. Gnathopod 2 propodus elongated, oval shape, without projections on the posterior margin; palm smooth, slope oblique, strongly inclined, longer than posterior margin; dactylus long, exceeding half length of propodus. Uropod 1 inner ramus of male with one curved seta and four apical cuspidate setae. Uropod 3 peduncle with one cuspidate seta distally; ramus with one cuspidate seta and two to three distal simple setae.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFDEFF80FF0CFE02DB396F9D.taxon	description	Description of male. (Figs 2 E and 7 A). Mean body length: 5.4 ± 0.9 mm (n = 3); mean cephalothorax length: 0.4 ± 0.08 mm. Body surface smooth, epimeral plates not acuminate. Eyes absent. Antenna 1 (Fig. 7 B) longer than antenna 2; total length reaches fourth pereonite; peduncle not surpassing first pereonite; flagellum with 11 to 13 articles, each with one or two aesthetascs after article 2. Antenna 2 (Fig. 7 C) total length reaches fourth segment; peduncle not surpassing first pereonite; flagellum with eight to ten articles. Upper lip (Fig. 7 D) margin rounded, distal border covered by setules on dorsal and ventral faces. Mandible (Fig. 7 E) basic amphipodan (in the sense of Watling 1993), but without palp; incisor toothed; left lacinia mobilis with five teeth and setal row with two pappose setae, right mandible with three pappose setae; molar process broad and cylindrical with accessory seta. Lower lip (Fig. 7 F) lobes rounded, with setules on dorsal and ventral faces. Maxilla 1 (Fig. 7 G) inner plate shorter than outer plate, with two distal papposerrate setae and several setules on the margin. Outer plate with nine serrate setae; palp short, uniarticulated with one distal setae. Maxilla 2 (Fig. 7 H) inner and outer plate of similar sizes, inner ramus with two papposerrate setae, nine serrulate and several simple setae; outer plate with several simple setae and one long plumose seta distally. Maxilliped (Fig. 7 I) inner ramus with three cuspidate distal setae and several pappose and simple setae on the margin; outer ramus with simple setae and two serrate setae on the margin; palp with four articles with several simple setae; dactylus unguiform, shorter (1 / 2) than the article 3, with simple seta and distal nail. Gnathopod 1 (Fig. 8 A) subchelate; basis, ischium and merus with simple setae; carpus longer than wider, shorter than propodus, lobe posterior margin with polygonal pattern and one row of serrate seta; propodus length 1.5 times the width (rectangular), oval shape, without projections on the posterior margin, inner face with five serrate setae; dactylus claw-like with simple setae and one plumose seta (Fig. 8 B). Gnathopod 2 (Fig. 8 C) subchelate; basis, ischium and merus with simple setae on the posterior margin; carpus wider than longer, lobe posterior margin with polygonal pattern and one row of serrate setae; propodus elongated, oval shape, without projections on the posterior margin, length 1.4 times the width (rectangular); smooth palm, slope oblique, strongly inclined, palm 1.5 times longer than posterior margin, with 12 to 20 cuspidate setae with accessory seta; dactylus claw-like, long, exceeding half the length of the propodus (Fig. 8 D). Pereopods 3 to 7 (Figs. 8 E and 8 F) merus, carpus, propodus posterior margin with cluster of cuspidate setae with accessory seta; dactylus length 1 / 3 of propodus; propodus dorsal and distal margins with simple setae; pereopod 3 and 4 with similar sizes; pereopod 5 smaller than others; pereopod 6 smaller than pereopod 7; which is about 1.5 times greater than pereopod 3. Pleopods (Fig. 9 A) peduncle shorter than rami, with distal coupling spines; both rami with several plumose setae. Uropod 1 (Fig. 9 B) peduncle longer than rami, with four cuspidate setae with accessory seta; inner ramus with one curved seta and four apical cuspidate setae (Fig. 9 C); outer ramus with three cuspidate setae with accessory seta on inner margin, and apex with four cuspidate setae, one of them with accessory seta (U 1 about 5 times the length of U 3). Uropod 2 (Fig. 9 D) shorter than uropod 1; peduncle with four cuspidate setae with accessory seta on the margin; inner ramus longer than outer ramus with one or two cuspidate setae with accessory seta on the margin, apex with five cuspidate setae with accessory seta; outer ramus with three cuspidate setae with accessory seta, apex with three to four cuspidate setae, two of them with accessory seta. Uropod 3 (Fig. 9 E) shorter than uropod 2, peduncle longer than ramus, longer than wide with one distal cuspidate setae; ramus uniarticulated with one cuspidate setae and two to three distal simple setae. Telson (Fig. 9 F) longer than wider, with two long simple setae and three short simple setae laterally. Coxal gills sac-like present on pereonites 2 to 6. Sternal gills tubular present on pereonites 2 to 7. Female. Cephalothorax length: 0.4 mm, body length: 5.0 mm (n = 1). Gnathopod 1 (Fig. 10 A) smaller than gnathopod 2; carpus wider than longer, lobe posterior margin with polygonal pattern; propodus longer than wider, inner face with five serrate setae. Gnathopod 2 (Fig. 10 B) similar to gnathopod 1, slightly larger, with carpus wider than longer, lobe posterior margin with polygonal pattern; propodus rectangular, longer than wider, inner face with five serrate setae. Uropod 1 peduncle longer than rami, inner margin with five cuspidate setae with accessory seta; inner ramus with two apical cuspidate setae; outer ramus with two cuspidate setae with accessory seta on the margin and apex with four cuspidate setae (Fig. 10 C). Habitat and ecological considerations. Hyalella. formosa was found in an underground lake in Andorinhas Cave, located near Ponta Grossa city (Fig. 2 A). The cave is mainly composed of sandstone; its entrance is within a fracture covered by dense vegetation, while the surrounding is quite modified, especially due to agricultural activities (Fig. 2 B). The cave comprises an elliptical hall approximately 140 meters long, its floor is strongly declined (Fig. 1 D) and, in the deepest portion, there is the lake (Fig. 1 C) where the species was found (Spinardi & Lopes, 1990). Dozens of individuals were observed swimming in the water column. There is organic material composed by plant fragments that are flushed in from the outside by water during rainy periods. The water table appears to vary over the year so the configuration of the pond level may be variable depending on the season. Other fractures were observed in the region, although apparently not connected with the water table. Although the only possible habitat for this species appears to be Andorinhas Cave, it is quite likely that the distribution of the species is more extensive, comprising part of the water table in the area.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
03DF87D1FFDEFF80FF0CFE02DB396F9D.taxon	discussion	Remarks. The troglomorphic features found in H. formosa, such as the absence of eyes resembles H. caeca, H. imbya, H. anophthalma and H. muerta; and the antenna 1 being longer than antenna 2, resemble H. imbya and H. muerta. The propodus of gnathopod 2 with a smooth palm in H. formosa is similar to H. caeca and H. imbya, while in H. spelaea, H. anophthalma and H. muerta the palm is irregular. In addition H. formosa shares with H. imbya the strongly inclinated palm of gnathopod 2, the absence of denticles on the posterior margin of gnathopod 2 carpus (Fig. 23), the presence of one curved seta in uropod 1 and long pereopods 6 and 7. The new species has unique characteristics such as the shape of gnathopod 1 distinct from the hammer-shape type, which is present in all other species (González & Watling, 2003), as well as different numbers of setae on the gnathopod propodus, uropod 1 and 3.	en	Cardoso, Giovanna Monticelli, Araujo, Paula Beatriz, Bueno, Alessandra Angélica De Pádua, Ferreira, Rodrigo Lopes (2014): Two new subterranean species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from Brazil. Zootaxa 3814 (3): 353-368, DOI: 10.11646/zootaxa.3814.3.3
