taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03DC776A9122FFA4FCF52E9094C7C057.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460606/files/figure.png	https://doi.org/10.5281/zenodo.5460606	Figure 2. Trachelolophos quadrinucleatus sp. nov. from life (A–D, F, G) and after protargol staining (E, H–J). A, typical individual; B, C, different body shapes; D, wedge-like tail; E, H, infraciliature of anterior end, showing circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; arrows point to anterior secant system forming on the left side of glabrous stripe; F, cortical granules arranged in line between somatic kineties; G, four macronuclei and two micronuclei forming a nuclear group; I, J, general infraciliature of the holotype specimen, marking single nuclear group, narrow glabrous stripe and anterior secant system forming on the left side of glabrous stripe (arrowheads). Abbreviations: BK, bristle kinety; CG, cortical granules; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear group; SK, somatic kineties. Scale bars: 500 μm in A–C; 200 μm in I, J.	Figure 2. Trachelolophos quadrinucleatus sp. nov. from life (A–D, F, G) and after protargol staining (E, H–J). A, typical individual; B, C, different body shapes; D, wedge-like tail; E, H, infraciliature of anterior end, showing circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; arrows point to anterior secant system forming on the left side of glabrous stripe; F, cortical granules arranged in line between somatic kineties; G, four macronuclei and two micronuclei forming a nuclear group; I, J, general infraciliature of the holotype specimen, marking single nuclear group, narrow glabrous stripe and anterior secant system forming on the left side of glabrous stripe (arrowheads). Abbreviations: BK, bristle kinety; CG, cortical granules; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear group; SK, somatic kineties. Scale bars: 500 μm in A–C; 200 μm in I, J.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9122FFA4FCF52E9094C7C057.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460608/files/figure.png	https://doi.org/10.5281/zenodo.5460608	Figure 3. Trachelolophos quadrinucleatus sp. nov. from life (A–G) and after protargol staining (H–M). A, typical individual; B, C, different body shapes; D, contracted cell; E, macronuclei forming a nuclear group and food vacuoles (arrows); F, wedge-shaped tail, noting food vacuoles including ingested algae (arrows); G, small colourless cortical granules arranged in line between somatic kineties (arrowheads); H, I, four macronuclei and two micronuclei forming a nuclear group; J, general infraciliature, marking single nuclear group; K, L, infraciliature of anterior end, showing circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; arrowheads point to anterior secant system forming on the left side of glabrous stripe; M, infraciliature of anterior portion indicating narrow glabrous stripe, bristle kinety and anterior secant system (arrowheads). Abbreviations: BK, bristle kinety; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear group; NU, nucleolus; SK, somatic kineties. Scale bars: 500 μm in A–D; 200 μm in J.	Figure 3. Trachelolophos quadrinucleatus sp. nov. from life (A–G) and after protargol staining (H–M). A, typical individual; B, C, different body shapes; D, contracted cell; E, macronuclei forming a nuclear group and food vacuoles (arrows); F, wedge-shaped tail, noting food vacuoles including ingested algae (arrows); G, small colourless cortical granules arranged in line between somatic kineties (arrowheads); H, I, four macronuclei and two micronuclei forming a nuclear group; J, general infraciliature, marking single nuclear group; K, L, infraciliature of anterior end, showing circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; arrowheads point to anterior secant system forming on the left side of glabrous stripe; M, infraciliature of anterior portion indicating narrow glabrous stripe, bristle kinety and anterior secant system (arrowheads). Abbreviations: BK, bristle kinety; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear group; NU, nucleolus; SK, somatic kineties. Scale bars: 500 μm in A–D; 200 μm in J.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9122FFA4FCF52E9094C7C057.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460604/files/figure.png	https://doi.org/10.5281/zenodo.5460604	Figure 1. Photographs of the sample site. A, an intertidal zone of a sandy beach in Qingdao (35°55′45″N, 120°12′59″E); B, an intertidal zone of a sandy beach in Daya Bay, Guangzhou (22°46′32″N, 114°40′13″E); C, a mangrove wetland in Zhanjiang (21°09′40″N, 110°25′38″E).	Figure 1. Photographs of the sample site. A, an intertidal zone of a sandy beach in Qingdao (35°55′45″N, 120°12′59″E); B, an intertidal zone of a sandy beach in Daya Bay, Guangzhou (22°46′32″N, 114°40′13″E); C, a mangrove wetland in Zhanjiang (21°09′40″N, 110°25′38″E).	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9123FFA2FCCC2B1F97FAC12D.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460612/files/figure.png	https://doi.org/10.5281/zenodo.5460612	Figure 4. Trachelolophos binucleatus sp. nov. from life (A–E) and after protargol staining (F–I). A, typical individual; B, C, different body shapes; D, two macronuclei and one micronucleus forming a nuclear group; E, small cortical granules arranged in line between somatic kineties; F, G, infraciliature of anterior end, indicating circumoral kinety, ciliary tuft, narrow glabrous strip and bristle kinety; arrow in F shows anterior secant system on the left side of glabrous stripe; H, I, general infraciliature of the holotype specimen, to show single nuclear group, narrow glabrous stripe, bristle kinety and anterior secant system (arrowheads). Abbreviations: BK, bristle kinety; CG, cortical granules; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronucleus; NG, nuclear group; SK, somatic kineties. Scale bars: 400 μm in A–C; 30 μm in F, G; 200 μm in H, I.	Figure 4. Trachelolophos binucleatus sp. nov. from life (A–E) and after protargol staining (F–I). A, typical individual; B, C, different body shapes; D, two macronuclei and one micronucleus forming a nuclear group; E, small cortical granules arranged in line between somatic kineties; F, G, infraciliature of anterior end, indicating circumoral kinety, ciliary tuft, narrow glabrous strip and bristle kinety; arrow in F shows anterior secant system on the left side of glabrous stripe; H, I, general infraciliature of the holotype specimen, to show single nuclear group, narrow glabrous stripe, bristle kinety and anterior secant system (arrowheads). Abbreviations: BK, bristle kinety; CG, cortical granules; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronucleus; NG, nuclear group; SK, somatic kineties. Scale bars: 400 μm in A–C; 30 μm in F, G; 200 μm in H, I.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9123FFA2FCCC2B1F97FAC12D.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460614/files/figure.png	https://doi.org/10.5281/zenodo.5460614	Figure 5. Trachelolophos binucleatus sp. nov. from life (A–F) and after protargol staining (G–M). A, typical individual; B, different body shape; C, slightly contracted cell; D, single nuclear group; E, F, small colourless cortical granules arranged in line between somatic kineties (arrowheads); G, general infraciliature, to show single nuclear group; H, two macronuclei forming a nuclear group; I–L, infraciliature of anterior end, indicating circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; M, infraciliature of anterior portion, noting narrow glabrous stripe and anterior secant system forming on the left side of it (arrowheads). Abbreviations: BK, bristle kinety; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; NG, nuclear group; SK, somatic kineties. Scale bars: 400 μm in A–C; 200 μm in G.	Figure 5. Trachelolophos binucleatus sp. nov. from life (A–F) and after protargol staining (G–M). A, typical individual; B, different body shape; C, slightly contracted cell; D, single nuclear group; E, F, small colourless cortical granules arranged in line between somatic kineties (arrowheads); G, general infraciliature, to show single nuclear group; H, two macronuclei forming a nuclear group; I–L, infraciliature of anterior end, indicating circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; M, infraciliature of anterior portion, noting narrow glabrous stripe and anterior secant system forming on the left side of it (arrowheads). Abbreviations: BK, bristle kinety; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; NG, nuclear group; SK, somatic kineties. Scale bars: 400 μm in A–C; 200 μm in G.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9123FFA2FCCC2B1F97FAC12D.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460604/files/figure.png	https://doi.org/10.5281/zenodo.5460604	Figure 1. Photographs of the sample site. A, an intertidal zone of a sandy beach in Qingdao (35°55′45″N, 120°12′59″E); B, an intertidal zone of a sandy beach in Daya Bay, Guangzhou (22°46′32″N, 114°40′13″E); C, a mangrove wetland in Zhanjiang (21°09′40″N, 110°25′38″E).	Figure 1. Photographs of the sample site. A, an intertidal zone of a sandy beach in Qingdao (35°55′45″N, 120°12′59″E); B, an intertidal zone of a sandy beach in Daya Bay, Guangzhou (22°46′32″N, 114°40′13″E); C, a mangrove wetland in Zhanjiang (21°09′40″N, 110°25′38″E).	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9123FFA2FCCC2B1F97FAC12D.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460608/files/figure.png	https://doi.org/10.5281/zenodo.5460608	Figure 3. Trachelolophos quadrinucleatus sp. nov. from life (A–G) and after protargol staining (H–M). A, typical individual; B, C, different body shapes; D, contracted cell; E, macronuclei forming a nuclear group and food vacuoles (arrows); F, wedge-shaped tail, noting food vacuoles including ingested algae (arrows); G, small colourless cortical granules arranged in line between somatic kineties (arrowheads); H, I, four macronuclei and two micronuclei forming a nuclear group; J, general infraciliature, marking single nuclear group; K, L, infraciliature of anterior end, showing circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; arrowheads point to anterior secant system forming on the left side of glabrous stripe; M, infraciliature of anterior portion indicating narrow glabrous stripe, bristle kinety and anterior secant system (arrowheads). Abbreviations: BK, bristle kinety; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear group; NU, nucleolus; SK, somatic kineties. Scale bars: 500 μm in A–D; 200 μm in J.	Figure 3. Trachelolophos quadrinucleatus sp. nov. from life (A–G) and after protargol staining (H–M). A, typical individual; B, C, different body shapes; D, contracted cell; E, macronuclei forming a nuclear group and food vacuoles (arrows); F, wedge-shaped tail, noting food vacuoles including ingested algae (arrows); G, small colourless cortical granules arranged in line between somatic kineties (arrowheads); H, I, four macronuclei and two micronuclei forming a nuclear group; J, general infraciliature, marking single nuclear group; K, L, infraciliature of anterior end, showing circumoral kinety, ciliary tuft, narrow glabrous stripe and bristle kinety; arrowheads point to anterior secant system forming on the left side of glabrous stripe; M, infraciliature of anterior portion indicating narrow glabrous stripe, bristle kinety and anterior secant system (arrowheads). Abbreviations: BK, bristle kinety; CK, circumoral kinety; CT, ciliary tuft; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear group; NU, nucleolus; SK, somatic kineties. Scale bars: 500 μm in A–D; 200 μm in J.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9125FFAFFCCC28C89636C05F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460618/files/figure.png	https://doi.org/10.5281/zenodo.5460618	Figure 6. Tracheloraphis similis from life (A–D) and after protargol staining (E–H). A, typical individual; B, wedgeshaped tail; C, four macronuclei and two micronuclei forming a nuclear group; D, distribution of cortical granules between somatic kineties and in glabrous stripe; E, F, infraciliature of anterior end, indicating circumoral kinety, brosse, bristle kinety, glabrous stripe and anterior secant system forming on left side of it (arrowheads); G, H, general infraciliature, noting two nuclear groups, each of which contains about four macronuclei; arrowheads point to secant system on left side of glabrous stripe. Abbreviations: B, brosse; BK, bristle kinety; CG, cortical granules; CK, circumoral kinety; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear groups; SK, somatic kineties. Scale bars: 400 μm in A, 150 μm in G, H.	Figure 6. Tracheloraphis similis from life (A–D) and after protargol staining (E–H). A, typical individual; B, wedgeshaped tail; C, four macronuclei and two micronuclei forming a nuclear group; D, distribution of cortical granules between somatic kineties and in glabrous stripe; E, F, infraciliature of anterior end, indicating circumoral kinety, brosse, bristle kinety, glabrous stripe and anterior secant system forming on left side of it (arrowheads); G, H, general infraciliature, noting two nuclear groups, each of which contains about four macronuclei; arrowheads point to secant system on left side of glabrous stripe. Abbreviations: B, brosse; BK, bristle kinety; CG, cortical granules; CK, circumoral kinety; GS, glabrous stripe; Ma, macronuclei; Mi, micronuclei; NG, nuclear groups; SK, somatic kineties. Scale bars: 400 μm in A, 150 μm in G, H.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9125FFAFFCCC28C89636C05F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460620/files/figure.png	https://doi.org/10.5281/zenodo.5460620	Figure 7. Tracheloraphis similis from life (A–F) and after protargol staining (G–L). A, typical individual; B, four macronuclei and two micronuclei forming a nuclear group; C, wedge-shaped tail; D, detailed view of middle region to show cytoplasmic granules; E, F, distribution of cortical granules (arrowheads) between somatic kineties and in glabrous stripe; G, general infraciliature, to show two nuclear groups, each of which contains about four macronuclei; H, L, infraciliature of anterior end, indicating circumoral kinety, brosse, glabrous stripe and bristle kinety; I, J, to show nuclear group including four (I) or six macronuclei (J) and micronucleus (I); K, infraciliature of mid-body portion, noting glabrous stripe bordered by bristle kinety. Abbreviations: B, brosse; BK, bristle kinety; CK, circumoral kinety; GS, glabrous stripe; Ma, macronuclei; Mi, micronucleus; NG, nuclear groups; NU, nucleolus; SK, somatic kineties. Scale bars: 400 μm in A; 150 μm in G.	Figure 7. Tracheloraphis similis from life (A–F) and after protargol staining (G–L). A, typical individual; B, four macronuclei and two micronuclei forming a nuclear group; C, wedge-shaped tail; D, detailed view of middle region to show cytoplasmic granules; E, F, distribution of cortical granules (arrowheads) between somatic kineties and in glabrous stripe; G, general infraciliature, to show two nuclear groups, each of which contains about four macronuclei; H, L, infraciliature of anterior end, indicating circumoral kinety, brosse, glabrous stripe and bristle kinety; I, J, to show nuclear group including four (I) or six macronuclei (J) and micronucleus (I); K, infraciliature of mid-body portion, noting glabrous stripe bordered by bristle kinety. Abbreviations: B, brosse; BK, bristle kinety; CK, circumoral kinety; GS, glabrous stripe; Ma, macronuclei; Mi, micronucleus; NG, nuclear groups; NU, nucleolus; SK, somatic kineties. Scale bars: 400 μm in A; 150 μm in G.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
03DC776A9125FFAFFCCC28C89636C05F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/5460624/files/figure.png	https://doi.org/10.5281/zenodo.5460624	Figure 8. ML tree inferred from the SSU rRNA gene sequences showing the positions of two newly sequenced species (arrow). Nodal support for branches in the ML and BI trees are marked in order. Nodes marked by filled circles indicate full support in both analyses (100% ML, 1.00 BI). Clades with a different topology between the two analyses are shown by an asterisk. Heterotrichea are selected as outgroups. All branches are drawn to scale. The scale bar corresponds to 2 substitutions per 100 nucleotide positions.	Figure 8. ML tree inferred from the SSU rRNA gene sequences showing the positions of two newly sequenced species (arrow). Nodal support for branches in the ML and BI trees are marked in order. Nodes marked by filled circles indicate full support in both analyses (100% ML, 1.00 BI). Clades with a different topology between the two analyses are shown by an asterisk. Heterotrichea are selected as outgroups. All branches are drawn to scale. The scale bar corresponds to 2 substitutions per 100 nucleotide positions.	2016-05-20	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo		Zenodo	biologists	Yan, Ying;Xu, Yuan;Al-Farraj, Saleh A.;Al-Rasheid, Khaled A. S.;Song, Weibo			
