identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03E787E8FF94FFAC88365B598154112E.text	03E787E8FF94FFAC88365B598154112E.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction	<div><p>DNA extraction and molecular analysis</p><p>Individual ticks were bisected longitudinally using sterile scalpels and washed with distilled water to remove residual ethanol. DNA was extracted using the commercial kit GeneJET Genomic DNA Purification Kit (Thermo Scientific, Lithuania) following manufacturer instructions. To confirm nymph tick species, ticks were analyzed through PCR amplification of a ~460 base pair (bp) fragment of the tick mitochondrial 16S rRNA gene (Mangold et al., 1998).</p><p>For the detection of microorganisms, extracted DNA was tested by a battery of PCR protocols targeting Rickettsia, Anaplasmataceae family organisms, Borrelia, Babesia and Hepatozoon spp., using specific primers and published protocols for each agent (Table 2). Molecular grade water was used as negative control in all PCR reactions. Positive controls used included Rickettsia parkeri strain Toledo, Ehrlichia canis isolate P1091, Borrelia anserina PL, Babesia bovis Paysandú, Hepatozoon sp. (Z78S, P1244), and Ixodes fuscipes S18IpH1. Five microliters of PCR products were separated by electrophoresis in a 1.5% agarose gel, stained with GoodView TM Nucleic Acid Stain (Beijing SBS Genetech Co., Ltd), and examined under UV transillumination. Amplicons of expected size were purified using GeneJET PCR purification kit and Sanger sequenced (Macrogen, Korea).</p></div>	https://treatment.plazi.org/id/03E787E8FF94FFAC88365B598154112E	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Castillero, Sergio Bermudez;Félix, María;Domínguez, Lillian;Abstract, José Venzal	Castillero, Sergio Bermudez, Félix, María, Domínguez, Lillian, Abstract, José Venzal (2025): Spotted fever group Rickettsia and Babesia odocoilei in hard ticks (Acari: Ixodidae) from wild areas in Panama. Acarological Studies 7 (2): 101-110, DOI: 10.47121/acarolstud.1726467, URL: https://doi.org/10.47121/acarolstud.1726467
