taxonID	type	description	language	source
03E787E8FF94FFAC88365B598154112E.taxon	description	For the detection of microorganisms, extracted DNA was tested by a battery of PCR protocols targeting Rickettsia, Anaplasmataceae family organisms, Borrelia, Babesia and Hepatozoon spp., using specific primers and published protocols for each agent (Table 2). Molecular grade water was used as negative control in all PCR reactions. Positive controls used included Rickettsia parkeri strain Toledo, Ehrlichia canis isolate P 1091, Borrelia anserina PL, Babesia bovis Paysandú, Hepatozoon sp. (Z 78 S, P 1244), and Ixodes fuscipes S 18 IpH 1. Five microliters of PCR products were separated by electrophoresis in a 1.5 % agarose gel, stained with GoodView TM Nucleic Acid Stain (Beijing SBS Genetech Co., Ltd), and examined under UV transillumination. Amplicons of expected size were purified using GeneJET PCR purification kit and Sanger sequenced (Macrogen, Korea).	en	Castillero, Sergio Bermudez, Félix, María, Domínguez, Lillian, Abstract, José Venzal (2025): Spotted fever group Rickettsia and Babesia odocoilei in hard ticks (Acari: Ixodidae) from wild areas in Panama. Acarological Studies 7 (2): 101-110, DOI: 10.47121/acarolstud.1726467, URL: https://doi.org/10.47121/acarolstud.1726467
