taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03E187EFFFB5AE4AFCA9BCA81C071C6C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552024/files/figure.png	https://doi.org/10.5281/zenodo.4552024	Fig. 1. Phase-contrast images of probable new species of microsporidium from fat body of female larva of Simulium kiewmaepanense, Doi Inthanon, Thailand. (A) Individual spores. (B) Developmental stages.	Fig. 1. Phase-contrast images of probable new species of microsporidium from fat body of female larva of Simulium kiewmaepanense, Doi Inthanon, Thailand. (A) Individual spores. (B) Developmental stages.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
03E187EFFFB5AE4AFCA9BCA81C071C6C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552026/files/figure.png	https://doi.org/10.5281/zenodo.4552026	Fig. 2. Species-specific chromosomal sequences of species from Doi Inthanon,Thailand. (A) IS base of Simulium tenebrosum cytospecies B (female larva), with bracket indicating limits of IS-33; C, centromere. (B) IIIL end of S. kiewmaepanense (male larva), showing IIIL-1 sequence (bracketed). (C) IIL distal half of S. undecimum (male larva) with bracket indicating limits of IIL-1 and enhanced, glassy band (+). (D) IIS of S. kiewmaepanense (male larva) showing IIS-1, IIS-2, IIS-3 sequence.The sequence involves two coincident breakpoints.Thus, for the hypothesis shown, inverting IIS-3 will create the e/g junction representing the IIS-1 break proximal to the centromere; when IIS-2 is then inverted, it creates the h/f junction representing the IIS-1 break distal to the centromere.The Simulium subgeneric standard sequence is, thus, obtained by alphabetically ordering the fragments indicated by the letters a–h; bu, bulge; C, centromere; RoB, ring of Balbiani.	Fig. 2. Species-specific chromosomal sequences of species from Doi Inthanon,Thailand. (A) IS base of Simulium tenebrosum cytospecies B (female larva), with bracket indicating limits of IS-33; C, centromere. (B) IIIL end of S. kiewmaepanense (male larva), showing IIIL-1 sequence (bracketed). (C) IIL distal half of S. undecimum (male larva) with bracket indicating limits of IIL-1 and enhanced, glassy band (+). (D) IIS of S. kiewmaepanense (male larva) showing IIS-1, IIS-2, IIS-3 sequence.The sequence involves two coincident breakpoints.Thus, for the hypothesis shown, inverting IIS-3 will create the e/g junction representing the IIS-1 break proximal to the centromere; when IIS-2 is then inverted, it creates the h/f junction representing the IIS-1 break distal to the centromere.The Simulium subgeneric standard sequence is, thus, obtained by alphabetically ordering the fragments indicated by the letters a–h; bu, bulge; C, centromere; RoB, ring of Balbiani.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
03E187EFFFB5AE49FCA9B93B19B21CB1.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552026/files/figure.png	https://doi.org/10.5281/zenodo.4552026	Fig. 2. Species-specific chromosomal sequences of species from Doi Inthanon,Thailand. (A) IS base of Simulium tenebrosum cytospecies B (female larva), with bracket indicating limits of IS-33; C, centromere. (B) IIIL end of S. kiewmaepanense (male larva), showing IIIL-1 sequence (bracketed). (C) IIL distal half of S. undecimum (male larva) with bracket indicating limits of IIL-1 and enhanced, glassy band (+). (D) IIS of S. kiewmaepanense (male larva) showing IIS-1, IIS-2, IIS-3 sequence.The sequence involves two coincident breakpoints.Thus, for the hypothesis shown, inverting IIS-3 will create the e/g junction representing the IIS-1 break proximal to the centromere; when IIS-2 is then inverted, it creates the h/f junction representing the IIS-1 break distal to the centromere.The Simulium subgeneric standard sequence is, thus, obtained by alphabetically ordering the fragments indicated by the letters a–h; bu, bulge; C, centromere; RoB, ring of Balbiani.	Fig. 2. Species-specific chromosomal sequences of species from Doi Inthanon,Thailand. (A) IS base of Simulium tenebrosum cytospecies B (female larva), with bracket indicating limits of IS-33; C, centromere. (B) IIIL end of S. kiewmaepanense (male larva), showing IIIL-1 sequence (bracketed). (C) IIL distal half of S. undecimum (male larva) with bracket indicating limits of IIL-1 and enhanced, glassy band (+). (D) IIS of S. kiewmaepanense (male larva) showing IIS-1, IIS-2, IIS-3 sequence.The sequence involves two coincident breakpoints.Thus, for the hypothesis shown, inverting IIS-3 will create the e/g junction representing the IIS-1 break proximal to the centromere; when IIS-2 is then inverted, it creates the h/f junction representing the IIS-1 break distal to the centromere.The Simulium subgeneric standard sequence is, thus, obtained by alphabetically ordering the fragments indicated by the letters a–h; bu, bulge; C, centromere; RoB, ring of Balbiani.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
03E187EFFFB6AE49FF09B84E1D5B1CF1.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552028/files/figure.png	https://doi.org/10.5281/zenodo.4552028	Fig. 3. Diagnostic sequences of chromosome IIIL for Simulium tenebrosum s. l. from Doi Inthanon,Thailand. (A, C, E) Simulium tenebrosum cytospecies A; female larvae. (B, D) Simulium tenebrosum cytospecies B. (A) Centromere and adjacent region of chromosome III; C, centromere; hb, heterozygous expression of heteroband 82; N.O., primary nucleolar organizer. (B) IIIL (female larva), showing fixed inversions IIIL-1, IIIL-8, IIIL-11, IIIL-13, IIIL-64 sequence with breakpoints indicated by arrows and brackets; C, centromere; cs, cup, and saucer marker. (C) IIIL end with heterozygous expression of secondary nucleolar organizer (2°N.O.96) and heteroband (hb); location of 2°N.O.94 indicated by arrow;inversion IIIL-96 indicated by bracket; IIIL-11 and IIIL-13 are present but not indicated. (D) IIIL end with heterozygous expression ofY-linked IIIL-10 (X0Y1) indicated by dashed bracket; inversion IIIL-64 indicated by bracket; IIIL-11 and IIIL-13 are present but not indicated. (E) IIIL, showing fixed inversions IIIL-1, IIIL-8, IIIL-11, IIIL-13, IIIL-96 sequence with breakpoints indicated by arrows and brackets; C, centromere; cs, cup, and saucer marker; N.O. primary nucleolar organizer.	Fig. 3. Diagnostic sequences of chromosome IIIL for Simulium tenebrosum s. l. from Doi Inthanon,Thailand. (A, C, E) Simulium tenebrosum cytospecies A; female larvae. (B, D) Simulium tenebrosum cytospecies B. (A) Centromere and adjacent region of chromosome III; C, centromere; hb, heterozygous expression of heteroband 82; N.O., primary nucleolar organizer. (B) IIIL (female larva), showing fixed inversions IIIL-1, IIIL-8, IIIL-11, IIIL-13, IIIL-64 sequence with breakpoints indicated by arrows and brackets; C, centromere; cs, cup, and saucer marker. (C) IIIL end with heterozygous expression of secondary nucleolar organizer (2°N.O.96) and heteroband (hb); location of 2°N.O.94 indicated by arrow;inversion IIIL-96 indicated by bracket; IIIL-11 and IIIL-13 are present but not indicated. (D) IIIL end with heterozygous expression ofY-linked IIIL-10 (X0Y1) indicated by dashed bracket; inversion IIIL-64 indicated by bracket; IIIL-11 and IIIL-13 are present but not indicated. (E) IIIL, showing fixed inversions IIIL-1, IIIL-8, IIIL-11, IIIL-13, IIIL-96 sequence with breakpoints indicated by arrows and brackets; C, centromere; cs, cup, and saucer marker; N.O. primary nucleolar organizer.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
03E187EFFFB6AE49FF09B84E1D5B1CF1.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552026/files/figure.png	https://doi.org/10.5281/zenodo.4552026	Fig. 2. Species-specific chromosomal sequences of species from Doi Inthanon,Thailand. (A) IS base of Simulium tenebrosum cytospecies B (female larva), with bracket indicating limits of IS-33; C, centromere. (B) IIIL end of S. kiewmaepanense (male larva), showing IIIL-1 sequence (bracketed). (C) IIL distal half of S. undecimum (male larva) with bracket indicating limits of IIL-1 and enhanced, glassy band (+). (D) IIS of S. kiewmaepanense (male larva) showing IIS-1, IIS-2, IIS-3 sequence.The sequence involves two coincident breakpoints.Thus, for the hypothesis shown, inverting IIS-3 will create the e/g junction representing the IIS-1 break proximal to the centromere; when IIS-2 is then inverted, it creates the h/f junction representing the IIS-1 break distal to the centromere.The Simulium subgeneric standard sequence is, thus, obtained by alphabetically ordering the fragments indicated by the letters a–h; bu, bulge; C, centromere; RoB, ring of Balbiani.	Fig. 2. Species-specific chromosomal sequences of species from Doi Inthanon,Thailand. (A) IS base of Simulium tenebrosum cytospecies B (female larva), with bracket indicating limits of IS-33; C, centromere. (B) IIIL end of S. kiewmaepanense (male larva), showing IIIL-1 sequence (bracketed). (C) IIL distal half of S. undecimum (male larva) with bracket indicating limits of IIL-1 and enhanced, glassy band (+). (D) IIS of S. kiewmaepanense (male larva) showing IIS-1, IIS-2, IIS-3 sequence.The sequence involves two coincident breakpoints.Thus, for the hypothesis shown, inverting IIS-3 will create the e/g junction representing the IIS-1 break proximal to the centromere; when IIS-2 is then inverted, it creates the h/f junction representing the IIS-1 break distal to the centromere.The Simulium subgeneric standard sequence is, thus, obtained by alphabetically ordering the fragments indicated by the letters a–h; bu, bulge; C, centromere; RoB, ring of Balbiani.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
03E187EFFFB6AE49FF09B84E1D5B1CF1.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552031/files/figure.png	https://doi.org/10.5281/zenodo.4552031	Fig. 4. Expression and locations of secondary nucleolar organizer (2°N.O.) in Simulium tenebrosum cytospecies A from Doi Inthanon,Thailand; female larvae. (A) IIIS distal to the primary nucleolar organizer (N.O.); arrow indicates location of 2°N.O.; bl, blister; ca, capsule. (B) IIS from centromere (C) to ring of Balbiani (RoB); arrow indicates location of 2°N.O. (C) IS end with heterozygous expression of 2°N.O. (D) IL end; arrow indicates location of 2°N.O.; n, neck.	Fig. 4. Expression and locations of secondary nucleolar organizer (2°N.O.) in Simulium tenebrosum cytospecies A from Doi Inthanon,Thailand; female larvae. (A) IIIS distal to the primary nucleolar organizer (N.O.); arrow indicates location of 2°N.O.; bl, blister; ca, capsule. (B) IIS from centromere (C) to ring of Balbiani (RoB); arrow indicates location of 2°N.O. (C) IS end with heterozygous expression of 2°N.O. (D) IL end; arrow indicates location of 2°N.O.; n, neck.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
03E187EFFFB7AE48FF09BA861B961CF6.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/4552035/files/figure.png	https://doi.org/10.5281/zenodo.4552035	Fig. 5. COI barcoding tree of representatives of the Simulium tuberosum species group in Southeast Asia. Bootstrap values are given on the branches.The horizontal bar with 0.1 above indicates substitutions per site.	Fig. 5. COI barcoding tree of representatives of the Simulium tuberosum species group in Southeast Asia. Bootstrap values are given on the branches.The horizontal bar with 0.1 above indicates substitutions per site.	2019-05-17	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn		Zenodo	biologists	Adler, Peter H.;Srisuka, Wichai;Low, Van Lun;Takaoka, Hiroyuki;Saeung, Atiporn			
