taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03FBF116846AFFEDFD89FDF16946FBC5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710260/files/figure.png	https://doi.org/10.5281/zenodo.7710260	Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm.	Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116846AFFEDFD89FDF16946FBC5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710262/files/figure.png	https://doi.org/10.5281/zenodo.7710262	Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm.	Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116846AFFEDFD89FDF16946FBC5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710264/files/figure.png	https://doi.org/10.5281/zenodo.7710264	Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.	Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116846AFFEDFD89FDF16946FBC5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710266/files/figure.png	https://doi.org/10.5281/zenodo.7710266	Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm.	Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116846AFFEDFD89FDF16946FBC5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710268/files/figure.png	https://doi.org/10.5281/zenodo.7710268	Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm.	Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116846AFFEDFD89FDF16946FBC5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710270/files/figure.png	https://doi.org/10.5281/zenodo.7710270	Fig. 6. Exanthemachrysis fresneliae Véron sp. nov. (AC35), viroplasm, TEM images (Fix 1). A. Benthic non-motile cell embedded in M; parietal C lobes with helicoidal arrangement of the TH lamellae. Cytoplasmic assembly of pentahedral virus (V). B. Viral particles scattered in the cytoplasm. Scale bars: A = 0.2 µm; B = 50 nm.	Fig. 6. Exanthemachrysis fresneliae Véron sp. nov. (AC35), viroplasm, TEM images (Fix 1). A. Benthic non-motile cell embedded in M; parietal C lobes with helicoidal arrangement of the TH lamellae. Cytoplasmic assembly of pentahedral virus (V). B. Viral particles scattered in the cytoplasm. Scale bars: A = 0.2 µm; B = 50 nm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEFFD8BFBEF6E92F9CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710260/files/figure.png	https://doi.org/10.5281/zenodo.7710260	Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm.	Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEFFD8BFBEF6E92F9CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710262/files/figure.png	https://doi.org/10.5281/zenodo.7710262	Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm.	Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEFFD8BFBEF6E92F9CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710264/files/figure.png	https://doi.org/10.5281/zenodo.7710264	Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.	Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEFFD8BFBEF6E92F9CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710266/files/figure.png	https://doi.org/10.5281/zenodo.7710266	Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm.	Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEFFD8BFBEF6E92F9CC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710268/files/figure.png	https://doi.org/10.5281/zenodo.7710268	Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm.	Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEBFDC9F9196B73FA0F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710272/files/figure.png	https://doi.org/10.5281/zenodo.7710272	Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm.	Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEBFDC9F9196B73FA0F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710274/files/figure.png	https://doi.org/10.5281/zenodo.7710274	Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm.	Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168465FFEBFDC9F9196B73FA0F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710276/files/figure.png	https://doi.org/10.5281/zenodo.7710276	Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm.	Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168461FFEAFDF1FA0D69BFFEF8.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710272/files/figure.png	https://doi.org/10.5281/zenodo.7710272	Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm.	Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168461FFEAFDF1FA0D69BFFEF8.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710274/files/figure.png	https://doi.org/10.5281/zenodo.7710274	Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm.	Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168461FFEAFDF1FA0D69BFFEF8.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710276/files/figure.png	https://doi.org/10.5281/zenodo.7710276	Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm.	Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168460FFF6FDA0FE7969CDFEE5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710278/files/figure.png	https://doi.org/10.5281/zenodo.7710278	Fig. 10. Pavlova sp. AC248, motile cells, LM images. Side views of curved oblong (A), ovoid (B) and spherical (C) free-swimming cells with conspicuous S-shaped AF emerging apically. Greenish brown parietal C (black arrows) with an E and a posterior bulging PY. Vacuolar crystals of barium sulphate (white arrows). Scale bars = 10 µm.	Fig. 10. Pavlova sp. AC248, motile cells, LM images. Side views of curved oblong (A), ovoid (B) and spherical (C) free-swimming cells with conspicuous S-shaped AF emerging apically. Greenish brown parietal C (black arrows) with an E and a posterior bulging PY. Vacuolar crystals of barium sulphate (white arrows). Scale bars = 10 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168460FFF6FDA0FE7969CDFEE5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710280/files/figure.png	https://doi.org/10.5281/zenodo.7710280	Fig. 11.Pavlova sp. AC248, chloroplast details, TEM images (Fix 1). A. Benthic colony of non-motile cells surrounded by a single, loose, non-layered M (white stars); single cup-shaped parietal C. B. Longitudinal section of a flagellate cell with parallel arrangement of TH in the C. Posterior PY protruding from the centre of the C, opposite a F, with an E at the tip of the C on its inner face. C. Detail of parallel TH lamellae forming stacks of 4 or 5 ending at the beginning of the PY.D. Detail of F insertion area showing proximity of the intrachloroplastic E to the long flagellar root (white arrows). Scale bars: A–B = 2 µm; C–D = 200 nm.	Fig. 11.Pavlova sp. AC248, chloroplast details, TEM images (Fix 1). A. Benthic colony of non-motile cells surrounded by a single, loose, non-layered M (white stars); single cup-shaped parietal C. B. Longitudinal section of a flagellate cell with parallel arrangement of TH in the C. Posterior PY protruding from the centre of the C, opposite a F, with an E at the tip of the C on its inner face. C. Detail of parallel TH lamellae forming stacks of 4 or 5 ending at the beginning of the PY.D. Detail of F insertion area showing proximity of the intrachloroplastic E to the long flagellar root (white arrows). Scale bars: A–B = 2 µm; C–D = 200 nm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168460FFF6FDA0FE7969CDFEE5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710282/files/figure.png	https://doi.org/10.5281/zenodo.7710282	Fig. 12. Pavlova sp. AC248, flagellar apparatus details of motile cells. A. SEM image of apical view of a swimming cell with a complete F apparatus emerging almost in the centre of a narrow, shallow pit: tomentose S-shaped AF, end-tapered PF and bipartite H inserted between both. Bulging PY on the opposite side. B–E. Negative staining images. B. Details of the base of the F apparatus showing the AF, PF with a tapered end, and H consisting of a proximal part of constant diameter and a distal part of subequal length and smaller diameter. C. Distal rounded part of the AF (with a blistering of the membrane) showing covering of long non-tubular hairs and regular arrangement of KS. D. Central part of the AF showing flat KS with a slight median constriction (black arrows) arranged in several more or less regular layers. E. Proximal part of the AF with multilayered KS, extending after a bare area in the immediate vicinity of the cell. Scale bars: A–B = 1 µm; C = 0.5 µm; D = 50 nm; E = 200 nm.	Fig. 12. Pavlova sp. AC248, flagellar apparatus details of motile cells. A. SEM image of apical view of a swimming cell with a complete F apparatus emerging almost in the centre of a narrow, shallow pit: tomentose S-shaped AF, end-tapered PF and bipartite H inserted between both. Bulging PY on the opposite side. B–E. Negative staining images. B. Details of the base of the F apparatus showing the AF, PF with a tapered end, and H consisting of a proximal part of constant diameter and a distal part of subequal length and smaller diameter. C. Distal rounded part of the AF (with a blistering of the membrane) showing covering of long non-tubular hairs and regular arrangement of KS. D. Central part of the AF showing flat KS with a slight median constriction (black arrows) arranged in several more or less regular layers. E. Proximal part of the AF with multilayered KS, extending after a bare area in the immediate vicinity of the cell. Scale bars: A–B = 1 µm; C = 0.5 µm; D = 50 nm; E = 200 nm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168460FFF6FDA0FE7969CDFEE5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710284/files/figure.png	https://doi.org/10.5281/zenodo.7710284	Fig. 13. Three Pavlova strains, details of the PY. A–E. TEM images. A. AC250, whole cell with a longitudinally sectioned C showing the E composed of osmiophilic clustered globules on its inner side and on the opposite side, the transversely sectioned campylotropous PY forming a slight hump on the cell surface and showing the narrow median space. (Fix 1). B. AC248, protruding campylotropous PY, longitudinally sectioned, completely surrounded by the C-membrane, bordered by periplastic ER on the cell surface and showing a narrow medial stromal space. (Fix 1). C. AC250, campylotropous PY, longitudinally sectioned emerging from the C towards the interior of the cell. (Fix 1). D. AC33, section of a palmelloid benthic cell surrounded by M with a cross sectioned campylotropous PY, in the middle of single cup-shaped parietal C, forming an ovoid bulge at the cell surface. (Original image, courtesy of C. Billard). E. AC248, detail of a cross section of the campylotropous pyrenoid attached to the plasma membrane (white arrows) showing the surrounding chloroplastic envelope lined on the inside by the periplastic ER (black arrows). (Fix 1). F. SEM image of AC248, postero-lateral view of a swimming cell with a complete F apparatus. PY bulging opposite the flagella with a tongue-like part projecting outwards from the cell. Scale bars: A = 1 µm; B = 20 nm; C = 0.5 µm; E = 200 nm; F = 2 µm.	Fig. 13. Three Pavlova strains, details of the PY. A–E. TEM images. A. AC250, whole cell with a longitudinally sectioned C showing the E composed of osmiophilic clustered globules on its inner side and on the opposite side, the transversely sectioned campylotropous PY forming a slight hump on the cell surface and showing the narrow median space. (Fix 1). B. AC248, protruding campylotropous PY, longitudinally sectioned, completely surrounded by the C-membrane, bordered by periplastic ER on the cell surface and showing a narrow medial stromal space. (Fix 1). C. AC250, campylotropous PY, longitudinally sectioned emerging from the C towards the interior of the cell. (Fix 1). D. AC33, section of a palmelloid benthic cell surrounded by M with a cross sectioned campylotropous PY, in the middle of single cup-shaped parietal C, forming an ovoid bulge at the cell surface. (Original image, courtesy of C. Billard). E. AC248, detail of a cross section of the campylotropous pyrenoid attached to the plasma membrane (white arrows) showing the surrounding chloroplastic envelope lined on the inside by the periplastic ER (black arrows). (Fix 1). F. SEM image of AC248, postero-lateral view of a swimming cell with a complete F apparatus. PY bulging opposite the flagella with a tongue-like part projecting outwards from the cell. Scale bars: A = 1 µm; B = 20 nm; C = 0.5 µm; E = 200 nm; F = 2 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF1168460FFF6FDA0FE7969CDFEE5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710264/files/figure.png	https://doi.org/10.5281/zenodo.7710264	Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.	Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116847CFFF6FDF9FE636F50FAB8.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710284/files/figure.png	https://doi.org/10.5281/zenodo.7710284	Fig. 13. Three Pavlova strains, details of the PY. A–E. TEM images. A. AC250, whole cell with a longitudinally sectioned C showing the E composed of osmiophilic clustered globules on its inner side and on the opposite side, the transversely sectioned campylotropous PY forming a slight hump on the cell surface and showing the narrow median space. (Fix 1). B. AC248, protruding campylotropous PY, longitudinally sectioned, completely surrounded by the C-membrane, bordered by periplastic ER on the cell surface and showing a narrow medial stromal space. (Fix 1). C. AC250, campylotropous PY, longitudinally sectioned emerging from the C towards the interior of the cell. (Fix 1). D. AC33, section of a palmelloid benthic cell surrounded by M with a cross sectioned campylotropous PY, in the middle of single cup-shaped parietal C, forming an ovoid bulge at the cell surface. (Original image, courtesy of C. Billard). E. AC248, detail of a cross section of the campylotropous pyrenoid attached to the plasma membrane (white arrows) showing the surrounding chloroplastic envelope lined on the inside by the periplastic ER (black arrows). (Fix 1). F. SEM image of AC248, postero-lateral view of a swimming cell with a complete F apparatus. PY bulging opposite the flagella with a tongue-like part projecting outwards from the cell. Scale bars: A = 1 µm; B = 20 nm; C = 0.5 µm; E = 200 nm; F = 2 µm.	Fig. 13. Three Pavlova strains, details of the PY. A–E. TEM images. A. AC250, whole cell with a longitudinally sectioned C showing the E composed of osmiophilic clustered globules on its inner side and on the opposite side, the transversely sectioned campylotropous PY forming a slight hump on the cell surface and showing the narrow median space. (Fix 1). B. AC248, protruding campylotropous PY, longitudinally sectioned, completely surrounded by the C-membrane, bordered by periplastic ER on the cell surface and showing a narrow medial stromal space. (Fix 1). C. AC250, campylotropous PY, longitudinally sectioned emerging from the C towards the interior of the cell. (Fix 1). D. AC33, section of a palmelloid benthic cell surrounded by M with a cross sectioned campylotropous PY, in the middle of single cup-shaped parietal C, forming an ovoid bulge at the cell surface. (Original image, courtesy of C. Billard). E. AC248, detail of a cross section of the campylotropous pyrenoid attached to the plasma membrane (white arrows) showing the surrounding chloroplastic envelope lined on the inside by the periplastic ER (black arrows). (Fix 1). F. SEM image of AC248, postero-lateral view of a swimming cell with a complete F apparatus. PY bulging opposite the flagella with a tongue-like part projecting outwards from the cell. Scale bars: A = 1 µm; B = 20 nm; C = 0.5 µm; E = 200 nm; F = 2 µm.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
03FBF116847CFFF6FDF9FE636F50FAB8.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7710286/files/figure.png	https://doi.org/10.5281/zenodo.7710286	Fig. 14. Key to the genera of the Pavlovales (+ = present, - = absent). Drawn by A. Defrance.	Fig. 14. Key to the genera of the Pavlovales (+ = present, - = absent). Drawn by A. Defrance.	2023-03-08	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier		Zenodo	biologists	Véron, Benoît;Rougier, Etienne;Taylor, Anthony;Goux, Didier			
