taxonID	type	description	language	source
038587BEB557BE74FF5CF93C4094F9AA.taxon	etymology	Etymology: — Referring to a new insect host in order Coleoptera.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
038587BEB557BE74FF5CF93C4094F9AA.taxon	materials_examined	Type: — CHINA. Guizhou: Qiannan Buyi and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E), on a dead ladybug (Coleoptera), 4 September 2021, Wanhao Chen, GZAC DY 09148 (holotype); ex-type living culture, DY 091481. Colonies on PDA reaching a diameter of 29 – 30 mm in 14 days at 25 ℃, convex, with white velutinate aerial mycelium, reverse yellowish to brownish, especially in the middle, margin entire, soluble pigment not produced. Vegetative hyphae branched, hyaline, smooth-walled, septate, 1.0 – 1.3 μm wide. Phialides produced on aerial hyphae, always solitary, aseptate, hyaline, smooth-walled, relatively slender, and tapering toward the tip, 22.5 – 30.0 × 1.3 – 1.9 μm. Conidia hyaline, ellipsoidal to globose, aseptate, smooth-walled, 1 - celled, 2.1 – 3.2 × 1.6 – 2.1 μm. Octahedral crystals absent. Sexual state not observed.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
038587BEB557BE74FF5CF93C4094F9AA.taxon	biology_ecology	Host: — Ladybug (Coleoptera). Additional strain examined: — CHINA. Guizhou: Qiannan Buyi and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E), on a dead ladybug (Coleoptera), 4 September 2021, Wanhao Chen, DY 091482 (living culture). Note: — Simplicillium neocoleopterorum was readily identified as Simplicillium based on the blast result in NCBI and the phylogenetic analysis of combined datasets (ITS, LSU, TEF) (Fig. 1), and clustered into an independent clade. When comparing the typical characteristics, S. neocoleopterorum has close relationship with S. araneae W. H. Chen, Y. F. Han, J. D. Liang & Z. Q. Liang, S. coleopterorum W. H. Chen, Y. F. Han, J. D. Liang & Z. Q. Liang, S. formicae D. P. Wei & K. D. Hyde and S. sinense X. Y. Yuan, Jing Z. Sun & H. W. Liu by its ellipsoidal to globose conidia and absent of octahedral crystals. However, S. neocoleopterorum was easily distinguished from these four species based on the phylogenetic analysis of combined datasets (ITS, LSU, TEF). Thus, the combined analysis of morphological characteristics and molecular phylogenetic results support S. neocoleopterorum as a new species.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
038587BEB551BE77FF5CF9CC45F8F932.taxon	etymology	Etymology: — Referring to its location at Mayao River Valley.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
038587BEB551BE77FF5CF9CC45F8F932.taxon	materials_examined	Type: — CHINA. Guizhou: Qiannan Buyi and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ’ 8.3748 ” N, 107 ° 23 ’ 16.96 ” E), on a dead spider (Araneae), 4 September 2021, Wanhao Chen, GZAC DY 0992 (holotype); ex-type living culture, DY 09921. Colonies on PDA reaching a diameter of 28 – 32 mm in 14 days at 25 ℃, convex, with white velutinate aerial mycelium, reverse yellowish, especially in the middle, margin entire, soluble pigment not produced. Vegetative hyphae branched, hyaline, smooth-walled, septate, 1.1 – 3.6 μm wide. Phialides produced on aerial hyphae, always solitary, aseptate, hyaline, smooth-walled, relatively slender, and tapering toward the tip, 13.0 – 28.1 × 1.5 – 2.6 μm. Conidia in small globose heads at the apex of the phialides, hyaline, ellipsoidal to globose, aseptate, smooth-walled, 1 - celled, 1.3 – 3.0 × 0.9 – 1.3 μm. Octahedral crystals absent. Sexual state not observed.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
038587BEB551BE77FF5CF9CC45F8F932.taxon	biology_ecology	Host: — Spider (Araneae). Additional strain examined: — CHINA. Guizhou: Qiannan Buyi and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E), on a dead spider (Araneae), 4 September 2021, Wanhao Chen, DY 09922 (living culture). Note: — Simplicillium vallense was easily identified as Simplicillium based on the blast result in NCBI and the phylogenetic analysis of combined datasets (ITS, LSU, TEF) (Fig. 1), and has a close relationship with S. neolepidopterorum W. H. Chen, Y. F. Han, J. D. Liang & Z. Q. Liang. S. vallense was easily distinguished from S. neolepidopterorum (phialide, 34.1 – 44.3 × 1.0 – 1.7 μm, absence of conidial heads) by its shorter phialides (13.0 – 28.1 × 1.5 – 2.6 μm) and presence of small globose conidial heads at the apex of the phialides. Jeewon and Hyde (2016) recommended that a minimum of> 1.5 % nucleotide differences in the ITS regions may be indicative of a new species. The pairwise dissimilarities of ITS sequences show 35 bp difference within 569 bp between S. vallense and S. neolepidopterorum (6.15 %). Thus, the morphological characteristics and molecular phylogenetic results support S. vallense as a new species.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
038587BEB552BE76FF5CF97B4173F90E.taxon	description	Strains examined: — CHINA. Guizhou: Qiannan Buyi and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E), on a dead spider (Araneae), 4 September 2021, Wanhao Chen, DY 09641 and DY 09642. Note: — Strains DY 09641 and DY 09642 were easily identified as Simplicillium based on the blast result in NCBI and the phylogenetic analysis of combined datasets (ITS, LSU, TEF) (Fig. 1), and clustered with S. formicae D. P. Wei & K. D. Hyde in a clade (100 / 1). Pairwise dissimilarities of ITS sequences show only 3 bp differences within 518 bp between strains DY 09641, DY 09642 and S. formicae (0.58 %). Thus, the molecular phylogenetic results strongly supported that strains DY 09641 and DY 09642 were S. formicae, which is a new species record in China.	en	Chen, Wan-Hao, Li, Dan, Shu, Hui-Lin, Liang, Jian-Dong, Han, Yan-Feng (2024): Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China. Phytotaxa 677 (3): 278-288, DOI: 10.11646/phytotaxa.677.3.7, URL: https://doi.org/10.11646/phytotaxa.677.3.7
