taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
0397E660DC5B831DFC6D45CBF118FCBA.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426121/files/figure.png	https://doi.org/10.5281/zenodo.8426121	Figure2. Drawings of Trimyema foissneri from life (A–D) and aħer protargol staining (E–I).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I).	Figure2. Drawings of Trimyema foissneri from life (A–D) and aħer protargol staining (E–I).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I).	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
0397E660DC5B831DFC6D45CBF118FCBA.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426123/files/figure.png	https://doi.org/10.5281/zenodo.8426123	Figure 3. Photomicrographs of Trimyema foissneri from life (A–C, J–M) and aħer protargol staining (D–I, N–º). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).G–I, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (A–C); 10 μm (G, P, º).	Figure 3. Photomicrographs of Trimyema foissneri from life (A–C, J–M) and aħer protargol staining (D–I, N–º). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).G–I, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (A–C); 10 μm (G, P, º).	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
0397E660DC5E831DFEB440FDF7ADFA2C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426125/files/figure.png	https://doi.org/10.5281/zenodo.8426125	Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C).	Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C).	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
0397E660DC5E831DFEB440FDF7ADFA2C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426127/files/figure.png	https://doi.org/10.5281/zenodo.8426127	Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm.	Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm.	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
0397E660DC5E8312FC6946EFF3F2F953.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426129/files/figure.png	https://doi.org/10.5281/zenodo.8426129	Figure 6. Drawings of Plagiopyla frontata from life (A–C, E, F) and after protargol staining (D, G–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, tangential section of the cortex, arrowhead marks straight extrusome, arrow indicates curved extrusome. C, extruded extrusomes with one end slightly inflated (red arrowheads). D, details of oral region, showing oral opening and buccal cavity (arrow). E, F, different cell shapes. G, dorsal view of a compressed cell, showing position of striated band, cytoproct (black arrow), dense ciliary rows (red arrow), and contractile vacuole pores (red arrowheads). H, ventral view to show somatic and oral kineties, arrowhead indicates gap between upper oral lip kineties and somatic kineties. I, dorsal view to show somatic kineties, arrow indicates striated band, arrowhead shows dense ciliary rows, double-arrowhead shows several irregularly and densely packed kinetosomes located near loop of striated band. Abbreviations: CV, contractile vacuole; Ma, macronucleus; SB, striated band. Scale bars: 35 μm (A, E, G, H); 10 μm (C).	Figure 6. Drawings of Plagiopyla frontata from life (A–C, E, F) and after protargol staining (D, G–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, tangential section of the cortex, arrowhead marks straight extrusome, arrow indicates curved extrusome. C, extruded extrusomes with one end slightly inflated (red arrowheads). D, details of oral region, showing oral opening and buccal cavity (arrow). E, F, different cell shapes. G, dorsal view of a compressed cell, showing position of striated band, cytoproct (black arrow), dense ciliary rows (red arrow), and contractile vacuole pores (red arrowheads). H, ventral view to show somatic and oral kineties, arrowhead indicates gap between upper oral lip kineties and somatic kineties. I, dorsal view to show somatic kineties, arrow indicates striated band, arrowhead shows dense ciliary rows, double-arrowhead shows several irregularly and densely packed kinetosomes located near loop of striated band. Abbreviations: CV, contractile vacuole; Ma, macronucleus; SB, striated band. Scale bars: 35 μm (A, E, G, H); 10 μm (C).	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
0397E660DC5E8312FC6946EFF3F2F953.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426131/files/figure.png	https://doi.org/10.5281/zenodo.8426131	Figure 7. Photomicrographs of Plagiopyla flontata in vivo (A–H) and aħer protargol staining (I–M). A, ventral view of a slightly compressed cell, arrow marks buccal cavity. B, representative individual, arrow shows oral opening, arrowhead indicates contractile vacuole.C, extruded extrusomes with one end slightly inflated (arrowheads).D, contractile vacuole pores (red arrowheads). E, ventral view of a compressed cell, arrows show straight extrusomes, arrowheads show curved extrusomes. F, details of dorsal side, arrowhead shows cytoproct. G, right side of a compressed cell showing striated band (arrowhead). H, details of extrusomes.I, macronucleus and micronucleus (white arrowhead). J, K, oral region, showing buccal cavity under different focal planes. L, M, ventral (L) and dorsal (M) views of the same individual, showing somatic and oral kineties, red arrow indicates gap between upper oral lip kineties and somatic kineties, black arrow shows striated band, black arrowhead shows dense ciliary rows, red arrowhead indicates several polykineties located near loop of striated band. Abbreviation: Ma, macronucleus. Scale bars: 35 μm.	Figure 7. Photomicrographs of Plagiopyla flontata in vivo (A–H) and aħer protargol staining (I–M). A, ventral view of a slightly compressed cell, arrow marks buccal cavity. B, representative individual, arrow shows oral opening, arrowhead indicates contractile vacuole.C, extruded extrusomes with one end slightly inflated (arrowheads).D, contractile vacuole pores (red arrowheads). E, ventral view of a compressed cell, arrows show straight extrusomes, arrowheads show curved extrusomes. F, details of dorsal side, arrowhead shows cytoproct. G, right side of a compressed cell showing striated band (arrowhead). H, details of extrusomes.I, macronucleus and micronucleus (white arrowhead). J, K, oral region, showing buccal cavity under different focal planes. L, M, ventral (L) and dorsal (M) views of the same individual, showing somatic and oral kineties, red arrow indicates gap between upper oral lip kineties and somatic kineties, black arrow shows striated band, black arrowhead shows dense ciliary rows, red arrowhead indicates several polykineties located near loop of striated band. Abbreviation: Ma, macronucleus. Scale bars: 35 μm.	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
0397E660DC5E8312FC6946EFF3F2F953.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8426135/files/figure.png	https://doi.org/10.5281/zenodo.8426135	Figure 8. Maximum likelihood (ML) tree inferred from the small subunit rRNA gene sequences, revealing the positions of the 21 newly obtained sequences (red font). Numbers near branches indicate ML bootstrap values and BI posterior probabilities. Black circles represent full support (ML/BI, 100/1.00) in both analyses. Disagreements in topology between ML and BI are marked with asterisks.The scale bar corresponds to 2 changes per 100 nucleotide positions.Abbreviation:sp = species.	Figure 8. Maximum likelihood (ML) tree inferred from the small subunit rRNA gene sequences, revealing the positions of the 21 newly obtained sequences (red font). Numbers near branches indicate ML bootstrap values and BI posterior probabilities. Black circles represent full support (ML/BI, 100/1.00) in both analyses. Disagreements in topology between ML and BI are marked with asterisks.The scale bar corresponds to 2 changes per 100 nucleotide positions.Abbreviation:sp = species.	2023-07-28	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong		Zenodo	biologists	Li, Ran;Zhuang, Wenbao;Feng, Xiaochen;Al-Farraj, Saleh A.;Schrecengost, Anna;Roưerova, Johana;Beinart, Roxanne A.;Hu, Xiaozhong			
