identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03927337FFADFFA60A66F111D4C9FC50.text	03927337FFADFFA60A66F111D4C9FC50.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ecklonia maxima	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> 2.2  Ecklonia maxima biomass </p>
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                  Mixed thalli of  E. maxima , harvested from  
                <a title="Search Plazi for locations around (long 16.867887/lat -29.253998)" href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=16.867887&amp;materialsCitation.latitude=-29.253998">Port Nolloth</a>
                 (−29.253997, 16.867888), South Africa  ,  were supplied by KelpX (Pty) Ltd. (Johannesburg, Gauteng, South Africa). Harvested thalli were first sundried, then cut into pieces (4 – 5 cm) before grinding to powder using a Platinum ® coffee grinder (Model: JC-CG150W, Johannesburg, Gauteng, South Africa). The powder was stored in a dark, dry place until further use . 
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	https://treatment.plazi.org/id/03927337FFADFFA60A66F111D4C9FC50	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Park, Research Article Yuchan;Malgas, Samkelo;Krause, Rui W. M.;Pletschke, Brett I.	Park, Research Article Yuchan, Malgas, Samkelo, Krause, Rui W. M., Pletschke, Brett I. (2024): Extraction and characterisation of sodium alginate from the Southern African seaweed Ecklonia maxima. Botanica Marina (Warsaw, Poland) 67 (5): 513-523, DOI: 10.1515/bot-2024-0011, URL: https://doi.org/10.1515/bot-2024-0011
03927337FFADFFA60A66F09DD45AF84E.text	03927337FFADFFA60A66F09DD45AF84E.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ecklonia maxima	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> 2.3 Extraction of sodium alginate from  E. maxima</p>
            <p>Sodium alginate was extracted according to a modified extraction process described by Lorbeer et al. (2015). Ground seaweed was depigmented in ethanol for 2 h with 99 % ethanol (1:2, solid:liquid) with gentle shaking; the process was repeated. The depigmented ground seaweed was dried at room temperature for 24 h, thereafter submerged in deionised water, and the pH adjusted to 1.0 using 2 M HCl in a solid-to-liquid ratio of 1:30 (w/v). This was left for 2 h at room temperature with a magnetic stirrer at 100 rpm on a magnetic stirrer plate. Thereafter, the supernatant and the residual biomass were separated through a cheesecloth, and the residual biomass was resuspended into a 0.5 M sodium carbonate solution at a solid-to-liquid ratio of 1:50. This solution was left overnight at a room temperature of 22 °C with a constant stirring with a magnetic stirrer at 100 rpm. The biomass was then sieved using a cheesecloth to separate it from the liquid containing the dissolved sodium alginate. This supernatant was made to a final ethanol concentration of 70 % (v/v) by adding 99 % ethanol and left for 1 h at room temperature to precipitate the sodium alginate. A sieve was used to separate the precipitated sodium alginate from the supernatant, which was washed with methanol and acetone (50 mL each) and then dried at room temperature of 22 °C overnight. The dried sodium alginate was stored in a closed container at room temperature until further use.</p>
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	https://treatment.plazi.org/id/03927337FFADFFA60A66F09DD45AF84E	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Park, Research Article Yuchan;Malgas, Samkelo;Krause, Rui W. M.;Pletschke, Brett I.	Park, Research Article Yuchan, Malgas, Samkelo, Krause, Rui W. M., Pletschke, Brett I. (2024): Extraction and characterisation of sodium alginate from the Southern African seaweed Ecklonia maxima. Botanica Marina (Warsaw, Poland) 67 (5): 513-523, DOI: 10.1515/bot-2024-0011, URL: https://doi.org/10.1515/bot-2024-0011
