taxonID	type	description	language	source
921287A7FFB2FFEC30D164F9AF42F8A4.taxon	materials_examined	Type: — UNITED STATES. Colorado: Boulder County, Little Gaynor Lake (40.12 ° N, 105.12 ° W), May 3 rd 2012. (holotype: COLO!, individual on slide JPK 9560, Kociolek Collection, here illustrated as Fig. 4. Isotype: AU!, slide MMDL 120503).	en	Li, Jing, Kociolek, J. Patrick, Gao, Yahui (2016): Chaetoceros coloradensis sp. nov. (Bacillariophyta, Chaetocerotaceae), a new inland species from Little Gaynor Lake, Colorado, North America. Phytotaxa 255 (3): 199-213, DOI: 10.11646/phytotaxa.255.3.2, URL: http://dx.doi.org/10.11646/phytotaxa.255.3.2
921287A7FFB2FFEC30D164F9AF42F8A4.taxon	etymology	Etymology: — Named for the state where the species was collected. Observations: — Over 100 vegetative cells have been measured. Frustules are solitary, delicate, containing only one chloroplast (Figs 2 – 3). The cells are rectangular in girdle view (Figs 3 – 5), sometimes nearly square (Fig. 3). Valve surfaces are flat (Figs 3 – 5), an anastomosing pattern of narrow ribs can be found on the valve face (Fig. 9). No processes are present on the valve (Fig. 9). Cells are 3.0 – 9.5 μm in the apical axis, 2.4 – 8.1 μm in the transapical axis, and 2.0 – 9.0 μm in the pervalvar axis. Very weak siliceous costae are ornamented on girdle bands (Fig. 10). Setae are thin, 3 – 4 times longer than apical axis. Setae on opposite valves have different orientation, with a 30 – 45 ° difference between these on opposite valves (Fig. 2). Spiral rows of rectangular pores, spiral rows of minute spines on setae (Fig. 13), and the pores, are smaller at the base of the setae (Fig. 11). Setae are closed at the end tip (Fig. 12). Nearly 300 resting spores have been measured. Resting spores are smooth, and form inside of vegetative cells (Fig. 5). Apical length of primary valve is 2.4 – 13.8 μm, width of the primary valve is 2.6 – 10.0 μm, height of primary valve is 3.6 – 6.2 μm. Length of secondary valve is 1.0 – 6.0 μm. Spore height ranges from 4.8 to 11.0 μm. The shape of resting spores is highly variable (Figs 27 – 35). The primary valve is convex, sometimes concave at the center (Fig. 28). The secondary valve sometimes has a chimney-like protrusion (Fig. 15). The secondary valve is not always substantially narrow when compared with the apical length of the primary valve (Figs 16, 27, 33). In addition, the secondary valve can be narrow and long (Fig. 34). The margin of the secondary valve is flat (Fig. 29) or concave (Fig. 30). Additionally, the secondary valve can be trapezoidal (Fig. 29) or inverted trapezoidal (Figs 28, 32) in shape. The secondary valve mantle might be elongated (Figs 17, 33, 35), or even be separated from the primary valve (Fig. 32). The primary and secondary valves can also be similar to one another (Fig. 31). There is a siliceous annulus at the margin of the secondary valve mantle (Fig. 15). Puncta are randomly distributed on the secondary valve mantle (Figs 15, 27). Phylogenetic analyses: — The ML and MrB for LSU have the same topologies (Fig. 24). In these trees, all solitary Chaetoceros species, including C. coloradensis, fall into clade V with high support (96 % ML / 100 % MrB). Within the clade V, C. coloradensis clusters with C. muelleri (strain GSL 5) and C. gracilis F. Schütt (1895: 42) with high support (100 % ML / 100 % MrB); while inside the small clade formed by these three species, C. muelleri clusters with C. gracilis and forms a smaller clade (ML 80 % / 99 % MrB), which suggests that C. muelleri is more closely related to C. gracilis in the LSU analysis. For SSU, the ML and MrB analyses also have the same topologies (Fig. 25). However, members of the section Simplicia do not fall into a single clade in the SSU analyses, indicating that the section Simplicia is not monophyletic. Although most Simplicia taxa are in clade IV (62 % ML / 87 % MrB), two strains of C. muelleri, two strains of C. calcitrans f. pumilus Takano (1968: 3) and two strains of C. calcitrans (Paulsen 1905: 6) Takano (1968: 1) fall outside of this clade. These two strains of C. muelleri form clade I with C. decipiens (Cleve 1873: 11) and C. cf. lorenzianus Grunow (1863: 157) with high support (100 % ML / 100 % MrB). The four strains of C. calcitrans and C. calcitrans f. pumilus form clade II with high support (100 % ML / 100 % MrB). Chaetoceros coloradensis falls into clade IV and forms a sister clade with the strain of C. muelleri collected from Great Salt Lake, but the support is low (60 % ML / 63 % MrB). The ML and MrB analyses generated the same topologies for the phylogenetic tree of LSU + SSU (Fig. 26). Solitary Chaetoceros species fall into clade V with high support (98 % ML / 100 % MrB). Chaetoceros coloradensis forms a small clade with C. muelleri (strain GSL 5) and C. gracilis with high support (100 % ML & MrB). Inside this small clade, C. coloradensis forms a clade with C. muelleri (strain GSL 5) (88 % ML and 100 % MrB). The analyses of LSU, SSU and LSU + SSU provide high support that the position of C. coloradensis is close to C. muelleri (strain GSL 5) isolated from Great Salt Lake. The molecular analyses also indicate C. coloradensis is one member of the clade composed by solitary Chaetoceros taxa.	en	Li, Jing, Kociolek, J. Patrick, Gao, Yahui (2016): Chaetoceros coloradensis sp. nov. (Bacillariophyta, Chaetocerotaceae), a new inland species from Little Gaynor Lake, Colorado, North America. Phytotaxa 255 (3): 199-213, DOI: 10.11646/phytotaxa.255.3.2, URL: http://dx.doi.org/10.11646/phytotaxa.255.3.2
