taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
A83CB427FFD4FFDBFCF2F9729FE2FEA4.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10994436/files/figure.png	https://doi.org/10.5281/zenodo.10994436	Fig. 2. A–F. Phialina pupula from life (A‒D, F) and after protargol impregnation (E). (A) Overview of a representative semi-contracted specimen. (B) Details of dumbbell-shaped inclusions from various views. (C) Extrusomes are rod-shaped and about 10 µm long. (D) Surface view showing cortical granulation. (E) Ciliary pattern. (F) Variability of body shape in extended, semi-contracted and contracted cells. CK – circumoral kinety; CV – contractile vacuole; DB – dorsal brush; DI – dumbbell-shaped inclusions; EB – extrusome bundle; EX – ex- trusomes; G – cortical granules; OB – oral bulge; MA – macronucleus; MI – micronucleus; SK – somatic kineties. Scale bars: 20 μm.	Fig. 2. A–F. Phialina pupula from life (A‒D, F) and after protargol impregnation (E). (A) Overview of a representative semi-contracted specimen. (B) Details of dumbbell-shaped inclusions from various views. (C) Extrusomes are rod-shaped and about 10 µm long. (D) Surface view showing cortical granulation. (E) Ciliary pattern. (F) Variability of body shape in extended, semi-contracted and contracted cells. CK – circumoral kinety; CV – contractile vacuole; DB – dorsal brush; DI – dumbbell-shaped inclusions; EB – extrusome bundle; EX – ex- trusomes; G – cortical granules; OB – oral bulge; MA – macronucleus; MI – micronucleus; SK – somatic kineties. Scale bars: 20 μm.	2019-12-31	Rajter, Ľubomír;Bourland, William;Vďačný, Peter		Zenodo	biologists	Rajter, Ľubomír;Bourland, William;Vďačný, Peter			
A83CB427FFD4FFDBFCF2F9729FE2FEA4.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10994438/files/figure.png	https://doi.org/10.5281/zenodo.10994438	Fig. 3. Phialina pupula from life under differential interference contrast (A–G) and bright field (H–M) illumination. (A) Overview of a semi-contracted specimen, showing the general body organization. The head is attached directly to the broadly fusiform trunk. Note that the contractile vacuole is located terminally due to the body contraction. The macronucleus is elliptical and situated slightly below the midbody. (B) Detail of the highly refractive dumbbell-shaped inclusions scattered throughout the cytoplasm. (C) A semi-contracted specimen, showing an accumulation of the dumbbell-shaped inclusions in the anterior body half. (D) Detail of the nuclear apparatus. The macronucle- us is elliptical, and the micronucleus is attached to the anterior pole of the macronucleus. (E) A contracted specimen, showing many refrac- tive, dumbbell-shaped inclusions scattered throughout the cytoplasm and an elliptical macronucleus accompanied by a single micronucleus. (F) A strongly squeezed specimen, showing the nuclear apparatus, multiple extrusome bundles and some lipid droplets scattered throughout the cytoplasm. Left inset shows optical section through the cortex (opposed arrowhead), containing inconspicuous elliptical granules. (G) Detail of a cytoplasmic rod-shaped extrusome. (H, J) Fusiform, slightly curved cells with narrowly rounded posterior body end. (I) A cylindrical cell. (K) An extended, fusiform exemplar with tail-like posterior end. (L) A sigmoid cell with narrowly rounded ends. (M) A semi-contracted, pyriform specimen with broadly rounded posterior body end. CV – contractile vacuole; DI – dumbbell-shaped inclusions; EB – extrusome bundles; EX – extrusomes; G – cortical granules; H – head; LD – lipid droplets; MA – macronucleus; MI – micronucleus; OB – oral bulge; T – trunk. Scale bars: 20 μm.	Fig. 3. Phialina pupula from life under differential interference contrast (A–G) and bright field (H–M) illumination. (A) Overview of a semi-contracted specimen, showing the general body organization. The head is attached directly to the broadly fusiform trunk. Note that the contractile vacuole is located terminally due to the body contraction. The macronucleus is elliptical and situated slightly below the midbody. (B) Detail of the highly refractive dumbbell-shaped inclusions scattered throughout the cytoplasm. (C) A semi-contracted specimen, showing an accumulation of the dumbbell-shaped inclusions in the anterior body half. (D) Detail of the nuclear apparatus. The macronucle- us is elliptical, and the micronucleus is attached to the anterior pole of the macronucleus. (E) A contracted specimen, showing many refrac- tive, dumbbell-shaped inclusions scattered throughout the cytoplasm and an elliptical macronucleus accompanied by a single micronucleus. (F) A strongly squeezed specimen, showing the nuclear apparatus, multiple extrusome bundles and some lipid droplets scattered throughout the cytoplasm. Left inset shows optical section through the cortex (opposed arrowhead), containing inconspicuous elliptical granules. (G) Detail of a cytoplasmic rod-shaped extrusome. (H, J) Fusiform, slightly curved cells with narrowly rounded posterior body end. (I) A cylindrical cell. (K) An extended, fusiform exemplar with tail-like posterior end. (L) A sigmoid cell with narrowly rounded ends. (M) A semi-contracted, pyriform specimen with broadly rounded posterior body end. CV – contractile vacuole; DI – dumbbell-shaped inclusions; EB – extrusome bundles; EX – extrusomes; G – cortical granules; H – head; LD – lipid droplets; MA – macronucleus; MI – micronucleus; OB – oral bulge; T – trunk. Scale bars: 20 μm.	2019-12-31	Rajter, Ľubomír;Bourland, William;Vďačný, Peter		Zenodo	biologists	Rajter, Ľubomír;Bourland, William;Vďačný, Peter			
A83CB427FFD4FFDBFCF2F9729FE2FEA4.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10994440/files/figure.png	https://doi.org/10.5281/zenodo.10994440	Fig. 4. Phialina pupula in the scanning electron microscope (SEM). (A) Detail of the anterior body half. The head is localized at the anterior body end and is attached directly to the trunk, as typical of the genus Phialina. The head is covered by very narrowly spaced cilia arranged in helically extending rows. Note that the cortex of the trunk is distinctly furrowed by slightly helically extending ciliary rows. According to protargol preparations, each somatic ciliary row has two to five brush dikinetids at its anterior end (see Fig. 2E). SEM observations show that the anterior basal body of a brush dikinetid bears a minute to short cilium or is unciliated, while the posterior basal body bears an or- dinary somatic cilium. Therefore, the brush is very difficult to recognize in the SEM and in vivo. (B) Detail of the anterior end of somatic ciliary rows, showing that the anterior basal body of a brush dikinetid bears a short cilium (arrowheads) or is unciliated. The posterior basal body of a brush dikinetid bears an ordinary somatic cilium. Such an inconspicuous brush is a typical feature of lacrymariids and also of the possibly related chaeneids. (C) Detail of a somatic ciliary row, showing a dikinetid (dividing basal bodies) followed by monokinetids that bear ordinary cilia. As typical for haptorians, the anterior cilium of dividing basal bodies is short and stump-like while the posterior cilium is ordinarily long. AC – anterior stump-like cilium of dividing basal bodies; G – tips of cortical granules; H – head; HC – head cilia; SC – somatic cilia; T – trunk.	Fig. 4. Phialina pupula in the scanning electron microscope (SEM). (A) Detail of the anterior body half. The head is localized at the anterior body end and is attached directly to the trunk, as typical of the genus Phialina. The head is covered by very narrowly spaced cilia arranged in helically extending rows. Note that the cortex of the trunk is distinctly furrowed by slightly helically extending ciliary rows. According to protargol preparations, each somatic ciliary row has two to five brush dikinetids at its anterior end (see Fig. 2E). SEM observations show that the anterior basal body of a brush dikinetid bears a minute to short cilium or is unciliated, while the posterior basal body bears an or- dinary somatic cilium. Therefore, the brush is very difficult to recognize in the SEM and in vivo. (B) Detail of the anterior end of somatic ciliary rows, showing that the anterior basal body of a brush dikinetid bears a short cilium (arrowheads) or is unciliated. The posterior basal body of a brush dikinetid bears an ordinary somatic cilium. Such an inconspicuous brush is a typical feature of lacrymariids and also of the possibly related chaeneids. (C) Detail of a somatic ciliary row, showing a dikinetid (dividing basal bodies) followed by monokinetids that bear ordinary cilia. As typical for haptorians, the anterior cilium of dividing basal bodies is short and stump-like while the posterior cilium is ordinarily long. AC – anterior stump-like cilium of dividing basal bodies; G – tips of cortical granules; H – head; HC – head cilia; SC – somatic cilia; T – trunk.	2019-12-31	Rajter, Ľubomír;Bourland, William;Vďačný, Peter		Zenodo	biologists	Rajter, Ľubomír;Bourland, William;Vďačný, Peter			
A83CB427FFDCFFD4FF46F8B49955FB5E.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10994442/files/figure.png	https://doi.org/10.5281/zenodo.10994442	Fig. 5. Phylogeny based on the 18S rRNA gene and the ITS1-5.8S-ITS2 region of 80 litostomatean taxa and two armophoreans serving as outgroup (CON-lit alignment). Posterior probabilities for the Bayesian inference and bootstrap values for maximum likelihood were mapped onto the 50%-majority rule Bayesian consensus tree. Note that monophyly of the family Lacrymariidae is moderately to strongly statistically supported. Sequences in bold face were obtained during this study. The scale bar indicates five substitutions per one hundred nucleotide positions. For GenBank accession numbers, see Supplementary Table S3.	Fig. 5. Phylogeny based on the 18S rRNA gene and the ITS1-5.8S-ITS2 region of 80 litostomatean taxa and two armophoreans serving as outgroup (CON-lit alignment). Posterior probabilities for the Bayesian inference and bootstrap values for maximum likelihood were mapped onto the 50%-majority rule Bayesian consensus tree. Note that monophyly of the family Lacrymariidae is moderately to strongly statistically supported. Sequences in bold face were obtained during this study. The scale bar indicates five substitutions per one hundred nucleotide positions. For GenBank accession numbers, see Supplementary Table S3.	2019-12-31	Rajter, Ľubomír;Bourland, William;Vďačný, Peter		Zenodo	biologists	Rajter, Ľubomír;Bourland, William;Vďačný, Peter			
A83CB427FFDCFFD4FF46F8B49955FB5E.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10994444/files/figure.png	https://doi.org/10.5281/zenodo.10994444	Fig. 6. Phylogeny based on the 18S rRNA gene of 22 taxa from the family Lacrymariidae (18S-lac1 alignment). Note that the genus Phialina is paraphyletic and contains the polyphyletic genus Lacrymaria. Posterior probabilities for the Bayesian inference and bootstrap values for maximum likelihood were mapped onto the 50%-majority rule ML tree. Sequences in bold were obtained during this study. The scale bar indicates nine substitutions per one thousand nucleotide positions.	Fig. 6. Phylogeny based on the 18S rRNA gene of 22 taxa from the family Lacrymariidae (18S-lac1 alignment). Note that the genus Phialina is paraphyletic and contains the polyphyletic genus Lacrymaria. Posterior probabilities for the Bayesian inference and bootstrap values for maximum likelihood were mapped onto the 50%-majority rule ML tree. Sequences in bold were obtained during this study. The scale bar indicates nine substitutions per one thousand nucleotide positions.	2019-12-31	Rajter, Ľubomír;Bourland, William;Vďačný, Peter		Zenodo	biologists	Rajter, Ľubomír;Bourland, William;Vďačný, Peter			
A83CB427FFDCFFD4FF46F8B49955FB5E.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10994440/files/figure.png	https://doi.org/10.5281/zenodo.10994440	Fig. 4. Phialina pupula in the scanning electron microscope (SEM). (A) Detail of the anterior body half. The head is localized at the anterior body end and is attached directly to the trunk, as typical of the genus Phialina. The head is covered by very narrowly spaced cilia arranged in helically extending rows. Note that the cortex of the trunk is distinctly furrowed by slightly helically extending ciliary rows. According to protargol preparations, each somatic ciliary row has two to five brush dikinetids at its anterior end (see Fig. 2E). SEM observations show that the anterior basal body of a brush dikinetid bears a minute to short cilium or is unciliated, while the posterior basal body bears an or- dinary somatic cilium. Therefore, the brush is very difficult to recognize in the SEM and in vivo. (B) Detail of the anterior end of somatic ciliary rows, showing that the anterior basal body of a brush dikinetid bears a short cilium (arrowheads) or is unciliated. The posterior basal body of a brush dikinetid bears an ordinary somatic cilium. Such an inconspicuous brush is a typical feature of lacrymariids and also of the possibly related chaeneids. (C) Detail of a somatic ciliary row, showing a dikinetid (dividing basal bodies) followed by monokinetids that bear ordinary cilia. As typical for haptorians, the anterior cilium of dividing basal bodies is short and stump-like while the posterior cilium is ordinarily long. AC – anterior stump-like cilium of dividing basal bodies; G – tips of cortical granules; H – head; HC – head cilia; SC – somatic cilia; T – trunk.	Fig. 4. Phialina pupula in the scanning electron microscope (SEM). (A) Detail of the anterior body half. The head is localized at the anterior body end and is attached directly to the trunk, as typical of the genus Phialina. The head is covered by very narrowly spaced cilia arranged in helically extending rows. Note that the cortex of the trunk is distinctly furrowed by slightly helically extending ciliary rows. According to protargol preparations, each somatic ciliary row has two to five brush dikinetids at its anterior end (see Fig. 2E). SEM observations show that the anterior basal body of a brush dikinetid bears a minute to short cilium or is unciliated, while the posterior basal body bears an or- dinary somatic cilium. Therefore, the brush is very difficult to recognize in the SEM and in vivo. (B) Detail of the anterior end of somatic ciliary rows, showing that the anterior basal body of a brush dikinetid bears a short cilium (arrowheads) or is unciliated. The posterior basal body of a brush dikinetid bears an ordinary somatic cilium. Such an inconspicuous brush is a typical feature of lacrymariids and also of the possibly related chaeneids. (C) Detail of a somatic ciliary row, showing a dikinetid (dividing basal bodies) followed by monokinetids that bear ordinary cilia. As typical for haptorians, the anterior cilium of dividing basal bodies is short and stump-like while the posterior cilium is ordinarily long. AC – anterior stump-like cilium of dividing basal bodies; G – tips of cortical granules; H – head; HC – head cilia; SC – somatic cilia; T – trunk.	2019-12-31	Rajter, Ľubomír;Bourland, William;Vďačný, Peter		Zenodo	biologists	Rajter, Ľubomír;Bourland, William;Vďačný, Peter			
