identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
B732B027FFD1A35EFE583E292E32C391.text	B732B027FFD1A35EFE583E292E32C391.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Aphanius fasciatus (Valenciennes 1821)	<div><p>RESULTS</p><p>Morphological analysis</p><p>Canonical variate analysis (CVA) shows that differences exist between females and males morphology of A. fasciatus (Fig. 3A). These differences are displayed along the second axis, which explains 30.39% of global variance. Specimen shape of Ayata Lake is discriminated from those of the three other localities along the first axis, which represents 45.73% of global variance (Fig. 3A). The variation highlighted along axis 1 is related to landmarks 3, 9, 11, 12 and 14 and 3, 4, 8, 10, 12, 13 for axis 2. For a better appreciation of the shape differences, we expose transformation grids based on procrustes coordinates covariance matrix (Fig. 3B). Because of the overlapping of Mellah lagoon, Mellah Marsh and Bizerte lagoon specimens, we used discriminate function to obtain a shape pair comparisons female/male. Transformation grids that discriminate sexes within populations were also described (Appendix 1). Based on transformations grids, Ayata Lake fish have a shorter head length than the other populations and the end of the mouth position litters higher (see points 2 and 14 in figure 3B). The base of opercule seems to be larger for Ayata Lake specimens. We noted variations between sexes in point 9, which corresponds to the insertion of the anal fin (smaller base in Ayata Lake) and more distant from pelvic fin in Ayata Lake. We see a difference between males and females along axis 2 (Cv2) in points 3, 4, 5, 7, 8 and 9. (Fig. 3B). Males are a bit wider than females, from the anal part to the caudal part of the body.</p><p>Genetic analysis</p><p>The seven-enzymatic systems (MDH, GOT, PGI, PGM, EST, IDH, LDH) gave clear zymograms. Among fifteen identified loci, eleven were polymorphic (GOT m, GOT f, PGI m, PGI f, PGM-2, EST f1, EST f2, EST m, LDH m, LDH f, IDH f). The mean number of alleles per locus (Am) varies between 1.93 for Ayata Lake and 1.33 for Mellah Marsh (Tab. II).</p><p>The highest value for both allelic richness (Ar = 1.45) and private allele richness (Ap = 0.32) are noted for Ayata Lake sample, while Bizerte lagoon is nearly close to Mellah lagoon in allelic richness (Ar = 1.21). However, the lowest is observed in Mellah marsh (1.11 for allelic richness and 0.06 for private allele richness) (Tab. II). For the expected heterozygosity, the highest value is 0.1413 ± 0.1803 in Ayata Lake and the lowest one in Mellah marsh (He = 0.0294 ± 0.0644).</p><p>The estimation of Wright fixation index Fis, according to Weir and Cockerham in each sample, revealed a significant deviation from panmixia with Fis = 0.282 (0.050 -0.505, 95% CI) in Bizerte lagoon and Fis = 0.524 (0.345 -0.653, 95% CI) in Ayata Lake. The estimation of global Fst shows a high significant value (Fst = 0.1246, P = 1). <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">Removing</a> PGM-2 locus, this value decreases and becomes not significant (Fst = 0.015, P = 0.053). <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">We</a> also note that after a jacknife analysis, when removing <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">Ayata Lake</a> sample, we obtain a lower and not significant value of <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">Fst</a> (0.0064, P = 0.186). <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">Genetic</a> distances calculated among pairwise samples according to Reynolds et al. (1983) showed that the highest distances were between <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">Ayata Lake</a> and the three other populations. <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">The</a> same finding was observed with <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=-0.653&amp;materialsCitation.latitude=0.345" title="Search Plazi for locations around (long -0.653/lat 0.345)">Fst</a> values as shown in table III.</p><p>Neighbour-joining tree was drawn basing on genetic distances of Reynold (Reynolds et al., 1983). The tree differentiates the populations in two clusters. The first group, supported by a bootstrap value of 100, contains Bizerte lagoon with Mellah lagoon and Mellah marsh as sub clade. This clade shows also high relationships between the two samples of Mellah region. The second clade only includes Ayata lake samples (Fig. 4).</p></div>	https://treatment.plazi.org/id/B732B027FFD1A35EFE583E292E32C391	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Labbaci, Abdelkader;Chaoui, Lamya;Bahri-Sfar, Lilia;Hammami, Ibtissem;Kara, M. Hichem	Labbaci, Abdelkader, Chaoui, Lamya, Bahri-Sfar, Lilia, Hammami, Ibtissem, Kara, M. Hichem (2021): Morphogeometric and genetic variations among North African populations of the Mediterranean killifish Aphanius fasciatus (Valenciennes, 1821) from different habitats. Cybium 45 (3): 225-238, DOI: 10.26028/cybium/2021-453-007, URL: http://dx.doi.org/10.26028/cybium/2021-453-007
