taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491104/files/figure.png	https://doi.org/10.5281/zenodo.10491104	Fig. 1. Elution profile of polyamines in P. vulgaris nodules (A), roots (B) and leaves (C) standard polyamines (D). Polyamines were extracted and derivatized with dansyl chloride before subjecting to HPLC analysis.1,7-diaminoheptane was used as a internal standard (IS). Peaks X, Y and Z in panel A denotes unknown compounds. Peaks in panel D: Dap; diaminopropane, Put; putrescine, Cad; cadaverine, Spd; spermidine, Homspd; homospermidine, Spm; spermine.	Fig. 1. Elution profile of polyamines in P. vulgaris nodules (A), roots (B) and leaves (C) standard polyamines (D). Polyamines were extracted and derivatized with dansyl chloride before subjecting to HPLC analysis.1,7-diaminoheptane was used as a internal standard (IS). Peaks X, Y and Z in panel A denotes unknown compounds. Peaks in panel D: Dap; diaminopropane, Put; putrescine, Cad; cadaverine, Spd; spermidine, Homspd; homospermidine, Spm; spermine.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491106/files/figure.png	https://doi.org/10.5281/zenodo.10491106	Fig. 2. Free putrescine (Put), spermidine (Spd), spermine (Spm), homospermidine (Homspd) and cadaverine (Cad) content in nodules of P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	Fig. 2. Free putrescine (Put), spermidine (Spd), spermine (Spm), homospermidine (Homspd) and cadaverine (Cad) content in nodules of P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491108/files/figure.png	https://doi.org/10.5281/zenodo.10491108	Fig. 3. Free 4-aminobutylcadaverine (4-ABcad) content expressed in relative values (%) in nodules of P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Relative values expressed as % are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	Fig. 3. Free 4-aminobutylcadaverine (4-ABcad) content expressed in relative values (%) in nodules of P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Relative values expressed as % are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491110/files/figure.png	https://doi.org/10.5281/zenodo.10491110	Fig. 4. Soluble conjugated putrescine (Putc), spermidine (Spdc) and cadaverine (Cadc) content in nodules of P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	Fig. 4. Soluble conjugated putrescine (Putc), spermidine (Spdc) and cadaverine (Cadc) content in nodules of P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10568451/files/figure.png	https://doi.org/10.5281/zenodo.10568451	Fig. 5. Expression analyses of arginine decarboxylase (Adc), ornithine decarboxylase (Odc), spermidine synthase (Spds), spermine synthase (Spms) and S-adenosylmethionine decarboxylase (Samdc) in leaves (L), roots (R) and nodules (N) of P. vulgaris inoculated with R. tropici CIAT899. 10 µl of the RT-PCR product was loaded on each lane and separated by electrophoresis on 1% (w/v) agarose gel. Acting product was used as a loading control.	Fig. 5. Expression analyses of arginine decarboxylase (Adc), ornithine decarboxylase (Odc), spermidine synthase (Spds), spermine synthase (Spms) and S-adenosylmethionine decarboxylase (Samdc) in leaves (L), roots (R) and nodules (N) of P. vulgaris inoculated with R. tropici CIAT899. 10 µl of the RT-PCR product was loaded on each lane and separated by electrophoresis on 1% (w/v) agarose gel. Acting product was used as a loading control.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491112/files/figure.png	https://doi.org/10.5281/zenodo.10491112	Fig. 6. Quantitative RT-PCR analysis of arginine decarboxylase (Adc), ornithine decarboxylase (Odc), S-adenosylmethionine decarboxylase (Samdc) and spermidine synthase (Spds) transcripts in nodules of P. vulgaris inoculated with R. tropici CIAT899 treated with 0 and 100 mM NaCl for 3 and 7 days. Expression levels are normalized to the housekeeping gene Actin and are presented as relative to untreated plants. The data are expressed as the means and SE of three biological replications.	Fig. 6. Quantitative RT-PCR analysis of arginine decarboxylase (Adc), ornithine decarboxylase (Odc), S-adenosylmethionine decarboxylase (Samdc) and spermidine synthase (Spds) transcripts in nodules of P. vulgaris inoculated with R. tropici CIAT899 treated with 0 and 100 mM NaCl for 3 and 7 days. Expression levels are normalized to the housekeeping gene Actin and are presented as relative to untreated plants. The data are expressed as the means and SE of three biological replications.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491114/files/figure.png	https://doi.org/10.5281/zenodo.10491114	Fig. 7. Enzyme activities arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) in nodules of P. vulgaris inoculated with R. tropici CIAT899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	Fig. 7. Enzyme activities arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) in nodules of P. vulgaris inoculated with R. tropici CIAT899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 3). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491116/files/figure.png	https://doi.org/10.5281/zenodo.10491116	Fig. 8. Nitrogen fixation parameters: apparent nitrogenase activity (ANA), total nitrogenase activity (TNA), nitrogen fixation rate (NFR), and nodule fresh weight (NFW) in P. vulgaris inoculated with R.tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 8). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	Fig. 8. Nitrogen fixation parameters: apparent nitrogenase activity (ANA), total nitrogenase activity (TNA), nitrogen fixation rate (NFR), and nodule fresh weight (NFW) in P. vulgaris inoculated with R.tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 8). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
BE3DB645FFD47E32E053A663FA80FEEC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/10491118/files/figure.png	https://doi.org/10.5281/zenodo.10491118	Fig. 9. Plant dry weight (PDW) and root to shoot rate (RSR) in P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 8). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	Fig. 9. Plant dry weight (PDW) and root to shoot rate (RSR) in P. vulgaris inoculated with R. tropici CIAT 899 treated with 0 and 100 mM NaCl for 3 and 7 days. Date are mean ± SE (n = 8). Mean values followed by the same letter do not differ (P 6 0.05) using LSD test.	2014-11-30	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen		Zenodo	biologists	López-Gómez, Miguel;Cobos-Porras, Libertad;Hidalgo-Castellanos, Javier;Lluch, Carmen			
