taxonID	type	description	language	source
D17D163B8E79FF8FFF4EFBFFFEE1598E.taxon	materials_examined	Type: — BRAZIL. Bahia: Mulungu do Morro, estrada de Segredo para Bonito, 810 m, 12 ° 0 ’ 23.00 ” S, 41 ° 32 ’ 4.99 ” W, 30 August 1999, Carneiro-Torres 123 (holotype HUEFS!).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E79FF8FFF4EFBFFFEE1598E.taxon	diagnosis	Diagnosis: — Portulaca giuliettiae differs from P. hirsutissima in having seeds covered by grayish and granulated wax throughout the relief, the outline irregular or corrugated in “ U ” or “ T ”, not bifurcated, the cells without convex projections; pollen grains pantocolpate, synaperturate, clypeate, the colpes arranged in pentagons.	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E79FF8FFF4EFBFFFEE1598E.taxon	description	Description: — Herb succulent, decumbent (rarely erect), 5.0 − 28.0 cm tall, stems branched, green to purplish; axillary trichomes overlying the entire stem, abundant and conspicuous, whitish, wooly. Leaves sessile, leaf blades 0.5 − 1.4 cm × 0.3 − 0.6 mm, oblong-linear, sometimes purplish, adaxial surface flat, the abaxial one convex, midrib not evident, base rounded, apex acute, margin entire, trichomes covering both surfaces. Inflorescence as a terminal capitulum 3 − 9 - flowered, the fowers open one at a time. Flowers sessile; sepals 0.5 − 1.0 cm long, concave, apex acute, with hirsute trichomes along the margins and on the abaxial surface; petals 5, obcordate, yellow, pink or magenta apex emarginate; style and filaments yellow or pink; stamens numerous, anthers yellow; pollen grains pantocolpate, clypeate, synaperturate, colpes organized in pentagons, exine spiny with annulate perforations. Fruit capsule sessile, 2.5 − 5.0 × 2.0 – 3.0 mm, base conical, 0.3 − 0.5 mm length, operculum ovoid 1.5 − 3.0 × 1.0 − 3.0 mm; seeds reniform, more than 100 per fruit, 0.3 − 0.5 mm length, black, seeds covered by grayish and granulated wax throughout the relief, the outline irregular or corrugated in “ U ” or “ T ”, not bifurcated, the cells without convex projections.	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E79FF8FFF4EFBFFFEE1598E.taxon	etymology	Etymology: — The epithet honors Dr. Ana Maria Giulietti Harley, an important Botanist who encouraged taxonomic studies on various plant families in Brazil, including Portulacaceae. Taxonomical notes: — Portulaca giuliettiae (Fig. 2 A ‒ C) can be easily distinguished from P. hirsutissima mainly by micromorphological characters, including pollen grains pantocolpate clypeate, such colpes are organized in pentagons, the exine spiny with annulate perforations (Fig. 2 D and 4 A). The seeds (Fig. 2 E – F and 4 B) are also distinct, covered by grayish and granulated wax throughout the relief, the outline irregular or corrugated in “ U ” or “ T ”, not bifurcated, and such cells have not convex projections. On the other hand, the corolla coloration in this new species appears to be very variable, occurring in a gradient between magenta or intermediates (Figs. 1, and 1 D) to yellowish (Fig. 1 E), mainly in individuals from Bahia. We noted that the specimens collected in rocky habitat of campo rupestre vegetation from Chapada Diamantina region show often a yellowish coloration, while corolla pink to magenta frequently occur in plants growing on sandy soils at the same locality. These features were seen in individuals only a few meters away and molecular analyses could be useful to correctly interpret this morphological variation.	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E79FF8FFF4EFBFFFEE1598E.taxon	distribution	Distribution, habitat and conservation: — Portulaca giuliettiae occurs in Eastern Brazil, from Pernambuco to Rio de Janeiro, excluding Alagoas and Sergipe States (Fig. 5). It can be usually found in restinga, campo rupestre vegetation, as well as in open and ruderal places often growing on sandy, rocky or calcareous soils. According to the IUCN criteria (IUCN 2012), this species is here considered as NT (Near Threatened). Paratypes: — BRAZIL. Bahia: Palmeiras, 29 January 2015, Vieira 147, 150 (HUEFS); Morro do Chapéu, 15 January 2014, Vieira 5, 9 (HUEFS). Espírito Santo: Barra do Jacu, 12 February 2013, Sucre 4611 (RB); Nova Venécia, 14 January 2009, Fontana 578 (RB); Vitória, 24 January 1985, Heleodoro 265 (RB). Paraíba, Mata Redonda, 15 November 1954, Falcão 1130 (RB). Pernambuco, Serra do Catimbau, 11 November 2003, Souza 397 (HUEFS). Rio de Janeiro, Rio de Janeiro, 6 June 2003, Calvente 40 (RB); 13 November 2003, Braga 7265 (RB); 18 February 1951, Brade 20587 (RB).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E7CFF8FFF4EFCA9FF4A5C9A.taxon	materials_examined	Type: — BRAZIL. Goiás: São João d’Aliança, 3 km de Alto Paraíso (Sul), 14 º 42 ’ 30 S. 47 º 31 ’ 17 ” W, 12 March 2007, J. F. B. Pastore et al. 1788 (holotype HUEFS!).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E7CFF8FFF4EFCA9FF4A5C9A.taxon	diagnosis	Diagnosis: — Portulaca goiasensis differs from P. hirsutissima and P. giuliettiae in having the seeds covered by grayish and granulated wax only at the basis of the cell relief, the outline corrugated in “ U ”, bifurcated, the cells with convex projections; pollen grains pantocolpate, synaperturate, colpes arranged in a spiral.	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E7CFF8FFF4EFCA9FF4A5C9A.taxon	description	Description: — Herb succulent, decumbent (rarely erect), 7.0 − 10 cm tall, stem branched, green to purplish; axillary trichomes overlying the entire stem, abundant and conspicuous, whitish, wooly. Leaves sessile, leaf blades 0.5 − 1 cm × 0.2 − 0.4 mm, oblong-linear, sometimes purplish, adaxial surface flat, the abaxial convex, midrib not evident, base rounded, apex acute, margin entire, trichomes covering both surfaces. Inflorescence a terminal capitulum, 3 − 7 - flowered, the flowers open one at a time. Flowers sessile, sepals 0.4 − 1.0 cm long, with hirsute trichomes along the margins and abaxial surface, concave, apex acute; petals 5, obcordate, apex emarginate, yellow; style and filaments yellow, stamens numerous, anthers yellow; pollen grains pantocolpate, synaperturate, colpes arranged in spiral, exine spiny with annulate perforations. Fruit capsule sessile, 2.0 − 2.5 × ca. 2.0 mm, base conical 0.5 − 1.0 mm length, operculum ovoid 2.0 × 2.5 mm; seeds reniform, more than 100 per fruit, 0.2 − 0.4 mm length, black, covered by grayish and granulated wax only at the basis of the cell relief, the outline corrugated in “ U ”, bifurcated, the cells with convex projections.	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E7CFF8FFF4EFCA9FF4A5C9A.taxon	etymology	Etymology: — The epithet refers to the geographical distribution of this new species, here considered as endemic to the Chapada dos Veadeiros, Goiás, in central Brazil (Fig. 5). Taxonomical notes: — Portulaca goiasensis (Fig. 3 A ‒ C) is similar to P. giuliettiae and P. hirsutissima, based on macromorphological characters, both vegetative and floral. The few specimens known have exclusively yellow corolla (Fig. 3 E and 4 D). The new species can be clearly separate by its micromorphology, such pollen grains are pantocolpate and have synaperturate colpes in a spiral (Fig. 3 D and 4 C), represents a peculiar feature which is not been registered for Portulaca until now (Carolin 1987). The seeds of P. goiasensis are covered by grayish and granulated wax only at the basis of the cell relief, being the outline corrugated in “ U ”, bifurcated, and the cells with convex projections, which are also important features to distinguish this new species (Fig. 3 F).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E7CFF8FFF4EFCA9FF4A5C9A.taxon	distribution	Distribution, habitat and conservation: — We considered Portulaca goiasensis as a endemic spcies to cerrado and campo rupestre vegetations in Goiás State (central Brazil). The individuals were found in dirty fields near streams and rocky soils in three municipalities from the Chapada dos Veadeiros region (Cristalina, São João d’Aliança and Serra dos Cristais) (Fig. 5). Lacking further data, this species si to be considered as DD (Deficient Data) according to the IUCN criteria (IUCN 2012). Paratypes: — BRAZIL: Goiás: Serra dos Cristais, 3 March 1964, Irwin 13362 (RB). Cristalina, Irwin 12643 (NY).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E72FF83FF4EF8A3FA165F96.taxon	materials_examined	Type (lectotype here designated): — BRAZIL. Minas Gerais: without locality, 1816 – 1821, Saint-Hilaire s. n. (P- 00835689). Image of the lectotype available at https: // science. mnhn. fr / institution / mnhn / collection / p / item / p 00835689? listIndex = 10 & listCount = 3384. Isolectotypes at P- 00835688! (https: // science. mnhn. fr / institution / mnhn / collection / p / item / p 00835688? listIndex = 9 & listCount = 3384), P- 00835687! (https: // science. mnhn. fr / institution / mnhn / collection / p / item / p 00835687? listIndex = 8 & listCount = 3384), P- 00835686! (https: // science. mnhn. fr / institution / mnhn / collection / p / item / p 00835686? listIndex = 7 & listCount = 3384), MPU 010812! (https: // science. mnhn. fr / institution / um 2 / collection / mpu / item / mpu 010812).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E72FF83FF4EF8A3FA165F96.taxon	description	Description: — Herb succulent (therophyte), prostrate (rarely erect), 7.0 − 30 cm tall, stems branched, green to purplish; axillary trichomes overlying the entire stem, abundant and conspicuous, whitish, wooly. Leaves sessile, leaf blades 0.5 − 2 cm × 1.5 − 4.0 mm, oblong-linear, adaxial surface flat, the abaxial convex, midrib not evident, base rounded, apex acute, sometimes purplish, margin entire, trichomes covering all surfaces. Inflorescence a terminal capitulum, 4 − 7 - flowered, the flowers open one at a time. Flowers sessile, sepals 0.5 − 0.8 cm long, with hirsute trichomes along the margins and abaxial surface, concave, apex acute; petals 5, obcordate, apex emarginate, yellow; style and filaments yellow; similar to the petals coloration, stamens numerous, anther yellow; pollen grains pantoporate, with pores isolated and organized in a polyhedral shape, exine spiny with annulate perforations. Fruit capsule sessile, 2.0 − 3.0 × 1.5 – 3.0 mm, base conical, 0.2 − 1.0 mm length; operculum ovoid 1.5 − 3.5 × 1.0 – 2.5 mm; seeds reniform, more than 100 per fruit, 0.2 − 0.4 mm length, black, seeds covered by grayish and smooth wax throughout the relief, the outline irregular or corrugated in “ U ”, not bifurcated, the cells without convex projections. Typification: — Portulaca hirsutissima was described by Cambessèdes (1829) who reported “ in pascuis prope Bom Fim et Nossa Sñra da Penha (Minas Novas), necnon prope Tejuco (Certao). Ab indefesso Laruotte lecta ”. Coelho et al. (2010) cited as the holotype of this species a specimen preserved at P indicating “ Laruotte s. n. ”. However, Laruotte was his colleague during the fieldtrips in Brazil, and no material was assigned to him, getting all the samples under Saint Hilaire´s responsibility. We found at MPU (barcode 010812) and P (barcodes 00835686, 00835687, 00835688 and 00835689) five specimens collected in Minas Gerais during Saint Hilaire´s fieldtrips (1816 – 1821) bearing labels with original annotations: “ Penha ”, “ Bonfim ” and “ Tejuco ”. Three specimens (P- 00835686, P- 00835687, P- 00835688) bear the annotation “ syntypes ” (by A. Coelho in September 2005), while the P- 00835689 was labeled as “ lectotype ”. According to McNeill et al. (2012) any syntype was listed in the protologue, while Coelho et al. (2010) never formalized a lectotypification. According to the Art. 9.9 of ICN (McNeill et al. 2012), we here correct the Coelho’s statement and designated the specimen P- 00835689 as the lectotype for the name Portulaca hirsutissima. We also consider as isolectotypes the other specimens from P and MPU previosly cited by these authors as syntypes. Taxonomical notes: — Portulaca hirsutissima (Fig. 1 G – H) it was traditionally considered as a very polymorphic species. Coelho & Giulietti (2010) reported the corolla magenta and / or yellow in specimens of P. hirsutissima from Bahia, and corolla exclusively yellow to specimens from Minas Gerais and other states (Fig. 1 H). On the basis of the results obtained in the present paper, P. hirsutissima shows pantoporate pollen grains, such pores are isolated and organized in a polyhedral shape (Fig. 4 E). In addition, the seeds (Fig. 4 F) display the cells relief covered by a smooth wax deposition, and the outline is irregular or corrugated in “ U ” shape, not bifurcated and with convex projections (Fig. 5).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
D17D163B8E72FF83FF4EF8A3FA165F96.taxon	distribution	Distribution, habitat and conservation: — Samples of Rio de Janeiro, Espírito Santo, Bahia and Paraíba are to be referred to P. giuliettiae (pollen pantocolpate, seeds without convex projections), while those from Goiás, to P. goiasensis (with pollen grains with colpes spirally arranged and convex projections with wax only at the base). The specimens from the type collection, from Minas Gerais, and other samples with a similar micromorphology are identifiable as the actual Portulaca hirsutissima. Preferential habitat is cerrado environments, on rocky and sandy soild. This species is considered as VU (Vulnerable) B 1 b (ii, iii, iv), with an EOO of about 20000 km 2 according to the IUCN criteria (IUCN 2012). It is restricted to Minas Gerais State, being directly affected by deforestation in that region. Specimens examined: — BRAZIL. Minas Gerais: Diamantina, 11 March 2015, Cardoso et al. 3784, 3788 (HUEFS), Serra do Cipó, 5 February 2009, Zappi et al. 1713 (RB). Nova Belém, 22 April 2013, Oliveira 350 (RB).	en	Santos, Thaíla Vieira A., Paes Coelho, Alexa Araújo De O., Dórea, Marcos Da Costa, Dos Santos, Francisco De Assis R., Leite, Kelly Regina B., Oliveira, Reyjane Patrícia (2016): Micromorphological features revealing two new species of Portulaca (Portulacaceae) from Brazil, segregated from P. hirsutissima. Phytotaxa 270 (2): 103-115, DOI: 10.11646/phytotaxa.270.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.270.2.3
