taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
EA5087B31909FFB5FEC4FDC996E9F945.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541989/files/figure.png	https://doi.org/10.5281/zenodo.14541989	Figure 3. Schematic drawings of Clevelandella ananiasi from life (A) and protargol impregnation (B–D). A, ventral view of a typical individual. B, ventral view showing the oral apparatus and nuclear structures. C, ventral infraciliature. D, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, circumperistomial cilia; CV, contractile vacuole; HL, hyaline subcortical layer; K, karyophore; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; SK, somatic kineties. Scale bars:20 µm.	Figure 3. Schematic drawings of Clevelandella ananiasi from life (A) and protargol impregnation (B–D). A, ventral view of a typical individual. B, ventral view showing the oral apparatus and nuclear structures. C, ventral infraciliature. D, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, circumperistomial cilia; CV, contractile vacuole; HL, hyaline subcortical layer; K, karyophore; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; SK, somatic kineties. Scale bars:20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFB5FEC4FDC996E9F945.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541993/files/figure.png	https://doi.org/10.5281/zenodo.14541993	Figure 4. Clevelandella ananiasi sp. nov. from ASS. In vivo (A, B) and after protargol impregnation (C–G). A, ventral view showing karyophore (white arrowhead), contractile vacuole (asterisk), posterior extent of somatic cilia (black arrowhead), and cilia of circumperistomial kineties (black arrow). B, ventral view showing a more slender macronucleus, karyophore (white arrowhead), contractile vacuole (asterisk), and adoral membranelles (black arrowhead).C, ventral view showing excretory (black arrow). D, ventral infraciliature.E, dorsal infraciliature of same cell as (D). F, detail view of peristomial projection showing the peristomial overture (black arrowhead) and cilia of the circumperistomial kineties (white arrowheads).G, detail view showing position of micronucleus (white arrow). Ma, macronucleus. Scale bars:20 µm (A–C), 10 µm (D–F), 5 µm (G).	Figure 4. Clevelandella ananiasi sp. nov. from ASS. In vivo (A, B) and after protargol impregnation (C–G). A, ventral view showing karyophore (white arrowhead), contractile vacuole (asterisk), posterior extent of somatic cilia (black arrowhead), and cilia of circumperistomial kineties (black arrow). B, ventral view showing a more slender macronucleus, karyophore (white arrowhead), contractile vacuole (asterisk), and adoral membranelles (black arrowhead).C, ventral view showing excretory (black arrow). D, ventral infraciliature.E, dorsal infraciliature of same cell as (D). F, detail view of peristomial projection showing the peristomial overture (black arrowhead) and cilia of the circumperistomial kineties (white arrowheads).G, detail view showing position of micronucleus (white arrow). Ma, macronucleus. Scale bars:20 µm (A–C), 10 µm (D–F), 5 µm (G).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFB5FEC4FDC996E9F945.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541995/files/figure.png	https://doi.org/10.5281/zenodo.14541995	Figure 5. Clevelandella ananiasi sp. nov. (from ASS) in the scanning electron microscope. A, ventral view showing anterior margin of peristomial overture (white arrowhead), cilia of adoral membranelles (black arrowhead), and cilia of circumperistomial kineties (black arrow). B, posteroventral view showing posterior end of the right suture (black arrow), the cytoproct with protruding cilia (white arrow), the inconspicuous left suture (black arrowhead), and the posterior end of the paroral membrane (white arrowhead). C, detail view of the peristomial overture showing adoral membranelles (black asterisk), cilia of the posterior end of the paroral membrane (black arrows), dikinetids of the posteriormost circumperistomial kinety (white arrowheads), and cilia of the circumperistomial kineties (white arrow). D, detail [same cell as (B)] showing the single file of basal bodies comprising the posterior part of the paroral membrane (white arrowhead), cilia of the paroral (black arrow), and the left suture (asterisk). Scale bars: 20 µm (A, B), 10 µm (C, D).	Figure 5. Clevelandella ananiasi sp. nov. (from ASS) in the scanning electron microscope. A, ventral view showing anterior margin of peristomial overture (white arrowhead), cilia of adoral membranelles (black arrowhead), and cilia of circumperistomial kineties (black arrow). B, posteroventral view showing posterior end of the right suture (black arrow), the cytoproct with protruding cilia (white arrow), the inconspicuous left suture (black arrowhead), and the posterior end of the paroral membrane (white arrowhead). C, detail view of the peristomial overture showing adoral membranelles (black asterisk), cilia of the posterior end of the paroral membrane (black arrows), dikinetids of the posteriormost circumperistomial kinety (white arrowheads), and cilia of the circumperistomial kineties (white arrow). D, detail [same cell as (B)] showing the single file of basal bodies comprising the posterior part of the paroral membrane (white arrowhead), cilia of the paroral (black arrow), and the left suture (asterisk). Scale bars: 20 µm (A, B), 10 µm (C, D).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFB5FED3F89C904AFA19.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541997/files/figure.png	https://doi.org/10.5281/zenodo.14541997	Figure 6. Schematic drawings of Clevelandella philipi sp. nov. from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view showing the oral apparatus and nuclear structures. C, ventral infraciliature.D, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties. Scale bars:20 µm.	Figure 6. Schematic drawings of Clevelandella philipi sp. nov. from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view showing the oral apparatus and nuclear structures. C, ventral infraciliature.D, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties. Scale bars:20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFB5FED3F89C904AFA19.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541999/files/figure.png	https://doi.org/10.5281/zenodo.14541999	Figure 7. Clevelandella philipi sp. nov., in vivo (A, B) and after protargol impregnation (C–I). From STH (A–G) and ASS (H, I). A, ventral view showing thick, hyaline cortical layer (between black arrowheads), cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B, ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A, B). C, ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D, dorsal view showing infraciliature, ciliated circumperistomial kineties (black arrow), looping kineties of peristomial projection (white arrow), and cytoproct (black arrowhead).E, ventral view showing left-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F, dorsal view showing straight somatic kineties (white asterisk), disordered basal bodies on left protuberance (white arrow), and paroral membrane (black arrowhead). G, dorsal view showing micronucleus (white arrowhead). H, dorsal view showing straight dorsal somatic kineties (asterisk), proximal margin of the peristomial overture (white arrow), and looping kineties of peristomial projection (black arrowhead). I, detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer left file (white arrowhead) of basal bodies. Ma, macronucleus. Scale bars:20 µm (A–H) 10 µm (I).	Figure 7. Clevelandella philipi sp. nov., in vivo (A, B) and after protargol impregnation (C–I). From STH (A–G) and ASS (H, I). A, ventral view showing thick, hyaline cortical layer (between black arrowheads), cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B, ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A, B). C, ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D, dorsal view showing infraciliature, ciliated circumperistomial kineties (black arrow), looping kineties of peristomial projection (white arrow), and cytoproct (black arrowhead).E, ventral view showing left-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F, dorsal view showing straight somatic kineties (white asterisk), disordered basal bodies on left protuberance (white arrow), and paroral membrane (black arrowhead). G, dorsal view showing micronucleus (white arrowhead). H, dorsal view showing straight dorsal somatic kineties (asterisk), proximal margin of the peristomial overture (white arrow), and looping kineties of peristomial projection (black arrowhead). I, detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer left file (white arrowhead) of basal bodies. Ma, macronucleus. Scale bars:20 µm (A–H) 10 µm (I).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFBBFC73FA529638F8A5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542001/files/figure.png	https://doi.org/10.5281/zenodo.14542001	Figure 8. Schematic drawings of Clevelandella hromadkai sp. nov. from life (A–D) and after protargol impregnation (E–G); long morphotype (A–C), short morphotype (D–G). A, left lateral view. B, right lateral view. C, (I–IV) cell outline in clockwise rotation around long axis. D, ventral view. E, ventral view.F, ventral infraciliature.G, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; K, karyophore; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars: 50 µm.	Figure 8. Schematic drawings of Clevelandella hromadkai sp. nov. from life (A–D) and after protargol impregnation (E–G); long morphotype (A–C), short morphotype (D–G). A, left lateral view. B, right lateral view. C, (I–IV) cell outline in clockwise rotation around long axis. D, ventral view. E, ventral view.F, ventral infraciliature.G, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; K, karyophore; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars: 50 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFBBFC73FA529638F8A5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542003/files/figure.png	https://doi.org/10.5281/zenodo.14542003	Figure 9. Clevelandella hromadkai sp. nov. short morph: from life (A–C, F–H) and after protargol impregnation (D, E, I). From ASS.A, left ventrolateral view showing micronucleus (white arrow), adoral membranelles (black arrow), and the posterior extent of ciliation on the right (black arrowhead) and left (white arrow head) margins. B, ventral view, (optical section) showing the cytostome (white arrowhead), contractile vacuole (black arrow) and margin of the peristomial overture (black arrowhead).C, left lateral view showing collecting vesicles of the contractile vacuole (white arrowheads), the micronucleus (white arrow) and the margin of the peristomial overture (black arrowhead).D,	Figure 9. Clevelandella hromadkai sp. nov. short morph: from life (A–C, F–H) and after protargol impregnation (D, E, I). From ASS.A, left ventrolateral view showing micronucleus (white arrow), adoral membranelles (black arrow), and the posterior extent of ciliation on the right (black arrowhead) and left (white arrow head) margins. B, ventral view, (optical section) showing the cytostome (white arrowhead), contractile vacuole (black arrow) and margin of the peristomial overture (black arrowhead).C, left lateral view showing collecting vesicles of the contractile vacuole (white arrowheads), the micronucleus (white arrow) and the margin of the peristomial overture (black arrowhead).D,	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFBBFC73FA529638F8A5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542005/files/figure.png	https://doi.org/10.5281/zenodo.14542005	Figure 10. Clevelandella hromadkai sp. nov.long morph from life (A–C) and after protargol impregnation (D–F). From ASS (A, E, F), and PAC (B–D). A, ventral view showing micronucleus (white arrow), left protuberance (black arrowhead), and karyophore (black arrow).B, left ventrolateral view showing peristomial overture (white arrow), collecting vesicles of contractile vacuole (white arrowheads), and the posterior extent of ciliation on the left ventrolateral margin (black arrowhead).C, right dorsolateral view showing contractile vacuole collecting vesicles (white arrowheads), the posterior extent of ciliation on the dorsolateral margin (black arrow), the micronucleus (white arrow), and the adoral membranelles (black arrowhead).D, detail showing micronucleus (white arrowhead) and karyophore (white arrow).E, left ventrolateral view showing micronucleus (white arrowhead).F, (same cell as E) showing posterior margin of left protuberance. Ma, macronucleus.Scale bars:20 µm (A–C, E, F), 10 µm (D).	Figure 10. Clevelandella hromadkai sp. nov.long morph from life (A–C) and after protargol impregnation (D–F). From ASS (A, E, F), and PAC (B–D). A, ventral view showing micronucleus (white arrow), left protuberance (black arrowhead), and karyophore (black arrow).B, left ventrolateral view showing peristomial overture (white arrow), collecting vesicles of contractile vacuole (white arrowheads), and the posterior extent of ciliation on the left ventrolateral margin (black arrowhead).C, right dorsolateral view showing contractile vacuole collecting vesicles (white arrowheads), the posterior extent of ciliation on the dorsolateral margin (black arrow), the micronucleus (white arrow), and the adoral membranelles (black arrowhead).D, detail showing micronucleus (white arrowhead) and karyophore (white arrow).E, left ventrolateral view showing micronucleus (white arrowhead).F, (same cell as E) showing posterior margin of left protuberance. Ma, macronucleus.Scale bars:20 µm (A–C, E, F), 10 µm (D).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31909FFBBFC73FA529638F8A5.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542007/files/figure.png	https://doi.org/10.5281/zenodo.14542007	Figure 11. Clevelandella hromadkai sp. nov. (from ASS) in the scanning electron microscope.A, right lateral view showing overall cell shape with concave dorsal margin (white arrowhead) and convex ventral margin (black arrowhead), ciliated anterior parts (black asterisk) and unciliated posterior parts (white asterisk) of somatic kineties, and prominent posterior peristomial projection (black arrow). B, posterodorsal view showing left posterior protuberance (black arrowhead) and peristomial overture (black arrow).C, left lateral view showing dorsoventral flattening, cilia protruding from the cytoproct (black arrowhead) on the left protuberance (white asterisk). D, posterodorsal view (down left) showing spiralling somatic kineties (white asterisk) and peristomial overture (black arrow) and right lateral view (black asterisk).E, posterodorsal view showing the cytoproct on the left protuberance (black arrow), the ciliated circumperistomial kineties (white arrowhead), and the adoral membranelles on the right wall of the infundibulum (black arrowhead). F, detail view of the left protuberance showing unciliated parts of kineties (white arrowheads) converging on the ciliated cytoproct (black arrowhead) and the fully ciliated dikinetids of the anterior body part (black arrow). Scale bars: 20 µm (A–E), 5 µm (F).	Figure 11. Clevelandella hromadkai sp. nov. (from ASS) in the scanning electron microscope.A, right lateral view showing overall cell shape with concave dorsal margin (white arrowhead) and convex ventral margin (black arrowhead), ciliated anterior parts (black asterisk) and unciliated posterior parts (white asterisk) of somatic kineties, and prominent posterior peristomial projection (black arrow). B, posterodorsal view showing left posterior protuberance (black arrowhead) and peristomial overture (black arrow).C, left lateral view showing dorsoventral flattening, cilia protruding from the cytoproct (black arrowhead) on the left protuberance (white asterisk). D, posterodorsal view (down left) showing spiralling somatic kineties (white asterisk) and peristomial overture (black arrow) and right lateral view (black asterisk).E, posterodorsal view showing the cytoproct on the left protuberance (black arrow), the ciliated circumperistomial kineties (white arrowhead), and the adoral membranelles on the right wall of the infundibulum (black arrowhead). F, detail view of the left protuberance showing unciliated parts of kineties (white arrowheads) converging on the ciliated cytoproct (black arrowhead) and the fully ciliated dikinetids of the anterior body part (black arrow). Scale bars: 20 µm (A–E), 5 µm (F).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31907FFBBFED9F8B597E5F869.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542011/files/figure.png	https://doi.org/10.5281/zenodo.14542011	Figure 12. Schematic drawings of Clevelandella klobasa sp. nov.from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual. B, ventral view.C, ventral infraciliature. D, dorsal infraciliature. AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties. Scale bars: 40 µm.	Figure 12. Schematic drawings of Clevelandella klobasa sp. nov.from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual. B, ventral view.C, ventral infraciliature. D, dorsal infraciliature. AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties. Scale bars: 40 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31907FFBBFED9F8B597E5F869.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542015/files/figure.png	https://doi.org/10.5281/zenodo.14542015	Figure 13. Clevelandella klobasa sp. nov.in vivo (A–C) and after protargol impregnation (D). From STH. A, left ventrolateral view showing thick hyaline subcortical layer (white arrowheads), cilia of adoral membranelles (black arrow), and margin of the peristomial overture (black arrowhead). B, left lateral view showing thick hyaline subcortical layer (white arrowheads), contractile vacuole (black arrowhead), and micronucleus (white arrow).C, optical section showing adoral membranelles (black arrow) and contractile vacuole (black arrowhead).D, left ventrolateral view (optical section) showing micronucleus (white arrow), adoral membranelles (black arrowhead), and paroral membrane (black arrow).Ma, macronucleus.Scale bars: 20 µm.	Figure 13. Clevelandella klobasa sp. nov.in vivo (A–C) and after protargol impregnation (D). From STH. A, left ventrolateral view showing thick hyaline subcortical layer (white arrowheads), cilia of adoral membranelles (black arrow), and margin of the peristomial overture (black arrowhead). B, left lateral view showing thick hyaline subcortical layer (white arrowheads), contractile vacuole (black arrowhead), and micronucleus (white arrow).C, optical section showing adoral membranelles (black arrow) and contractile vacuole (black arrowhead).D, left ventrolateral view (optical section) showing micronucleus (white arrow), adoral membranelles (black arrowhead), and paroral membrane (black arrow).Ma, macronucleus.Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31905FFB9FEFCFAAE90F5F9EB.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542019/files/figure.png	https://doi.org/10.5281/zenodo.14542019	Figure 14. Schematic drawings of Clevelandella sidi sp. nov.from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view. C, ventral infraciliature (holotype). D, dorsal infraciliature (holotype). AZM, adoral zone of membranelles; CV, contractile vacuole; CW, ciliary whorl of left protuberance; CYP, cytoproct; K, karyophore; Ma, macronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars: 20 µm.	Figure 14. Schematic drawings of Clevelandella sidi sp. nov.from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view. C, ventral infraciliature (holotype). D, dorsal infraciliature (holotype). AZM, adoral zone of membranelles; CV, contractile vacuole; CW, ciliary whorl of left protuberance; CYP, cytoproct; K, karyophore; Ma, macronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31905FFB9FEFCFAAE90F5F9EB.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542024/files/figure.png	https://doi.org/10.5281/zenodo.14542024	Figure 15. Clevelandella sidi sp.nov.in vivo (A, B) and after protargol impregnation (C–H). From SRU (A–H). A, ventral view showing contractile vacuole (white arrowhead) and cilia of adoral membranelles (white arrow). B, ventral view showing contractile vacuole (white arrowhead), adoral membranelles (black arrow), and elevated margin of the cytoproct (white arrow).C–E, the same cell in dorsal view: C, showing somatic infraciliature (asterisk), cytoproct (black arrowhead), and adoral membranelles (white arrow); D, showing cytoproct (black arrowhead) and dorsal kineties (white arrowhead); E, optical section showing excretory antrum within the left protuberance (black arrowheads). F, detail of the macronucleus showing the karyophore (white arrow). G, detail showing the micronucleus (white arrowhead). H, detail of the paroral membrane showing both files of equal length and extending nearly to the posterior end of the peristomial projection (white arrowheads), a feature unique to this species.Asterisk marks the anterior end. Ma, macronucleus.Scale bars:20 µm (A–D) 10 µm (E–G), 5 µm (H, I).	Figure 15. Clevelandella sidi sp.nov.in vivo (A, B) and after protargol impregnation (C–H). From SRU (A–H). A, ventral view showing contractile vacuole (white arrowhead) and cilia of adoral membranelles (white arrow). B, ventral view showing contractile vacuole (white arrowhead), adoral membranelles (black arrow), and elevated margin of the cytoproct (white arrow).C–E, the same cell in dorsal view: C, showing somatic infraciliature (asterisk), cytoproct (black arrowhead), and adoral membranelles (white arrow); D, showing cytoproct (black arrowhead) and dorsal kineties (white arrowhead); E, optical section showing excretory antrum within the left protuberance (black arrowheads). F, detail of the macronucleus showing the karyophore (white arrow). G, detail showing the micronucleus (white arrowhead). H, detail of the paroral membrane showing both files of equal length and extending nearly to the posterior end of the peristomial projection (white arrowheads), a feature unique to this species.Asterisk marks the anterior end. Ma, macronucleus.Scale bars:20 µm (A–D) 10 µm (E–G), 5 µm (H, I).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31905FFA0FC15F9F394C7FB7C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542026/files/figure.png	https://doi.org/10.5281/zenodo.14542026	Figure 16. Schematic drawings of Clevelandella fryntai sp. nov.from life (A, B) and after protargol impregnation (C–E). From SRU.A, ventral view. B, ventral view.C, ventral view.D, ventral infraciliature.E, dorsal infraciliature.AP, cytoplasmic granules, possibly amylopectin; AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; CW, ciliary whorl of left protuberance; K, karyophore; Ma, macronucleus, Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars:20 µm.	Figure 16. Schematic drawings of Clevelandella fryntai sp. nov.from life (A, B) and after protargol impregnation (C–E). From SRU.A, ventral view. B, ventral view.C, ventral view.D, ventral infraciliature.E, dorsal infraciliature.AP, cytoplasmic granules, possibly amylopectin; AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; CW, ciliary whorl of left protuberance; K, karyophore; Ma, macronucleus, Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars:20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31905FFA0FC15F9F394C7FB7C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542030/files/figure.png	https://doi.org/10.5281/zenodo.14542030	Figure 17.Clevelandella fryntai sp.nov.from life (A–E) and after protargol impregnation (F–K).From SRU.(A, B, F, G, K) slender morphotype, (C–E, H–J) broad morphotype.A, ventral view showing posterior extent of ciliation on the right margin (white arrowhead) and left margin (black arrowhead).B, dorsal view showing contractile vacuole (white arrowhead) and straight dorsal kineties (white arrow).C, ventral view (optical section) showing cytoplasmic granules, possibly amylopectin (black arrow), right kinetal suture (white arrowhead), adoral membranelles (black arrowhead),and cytoproct (white arrow).D, ventral view showing left-hand spiralling kineties (white arrowhead) and cilia of adoral membranelles (white arrow).E, ventral view showing the margin of the peristomial overture (black arrowhead) and bulbous left protuberance extending over left cell margin (white arrowhead).F, detail showing karyophore(black arrowhead).G, ventral view (optical section) showing the karyophore (black arrowhead), micronucleus (white arrow), and cytoproct (white arrowhead).H, dorsal view showing karyophore (black arrowheads), micronucleus (white arrowhead), and adoral membranelles (black arrow).I, ventral infraciliature showing left-hand spiralling kineties (asterisk) and looping kineties of the peristomial projection (white arrow). J, dorsal infraciliature showing straight dorsal kineties (asterisk). K, detail view showing diplostichomonad paroral (black arrow).Ma, macronucleus.Scale bars: 20 µm (A–E, G–J), 10 µm (F, K).	Figure 17.Clevelandella fryntai sp.nov.from life (A–E) and after protargol impregnation (F–K).From SRU.(A, B, F, G, K) slender morphotype, (C–E, H–J) broad morphotype.A, ventral view showing posterior extent of ciliation on the right margin (white arrowhead) and left margin (black arrowhead).B, dorsal view showing contractile vacuole (white arrowhead) and straight dorsal kineties (white arrow).C, ventral view (optical section) showing cytoplasmic granules, possibly amylopectin (black arrow), right kinetal suture (white arrowhead), adoral membranelles (black arrowhead),and cytoproct (white arrow).D, ventral view showing left-hand spiralling kineties (white arrowhead) and cilia of adoral membranelles (white arrow).E, ventral view showing the margin of the peristomial overture (black arrowhead) and bulbous left protuberance extending over left cell margin (white arrowhead).F, detail showing karyophore(black arrowhead).G, ventral view (optical section) showing the karyophore (black arrowhead), micronucleus (white arrow), and cytoproct (white arrowhead).H, dorsal view showing karyophore (black arrowheads), micronucleus (white arrowhead), and adoral membranelles (black arrow).I, ventral infraciliature showing left-hand spiralling kineties (asterisk) and looping kineties of the peristomial projection (white arrow). J, dorsal infraciliature showing straight dorsal kineties (asterisk). K, detail view showing diplostichomonad paroral (black arrow).Ma, macronucleus.Scale bars: 20 µm (A–E, G–J), 10 µm (F, K).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31905FFA0FC15F9F394C7FB7C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542032/files/figure.png	https://doi.org/10.5281/zenodo.14542032	Figure 18. Clevelandella fryntai sp.nov.in the scanning electron microscope.From SRU. (A, B) slender morphotype, (C–F) broad morphotype. A, dorsal view showing cytoproct on left protuberance (black arrowhead), anterior margin of peristomial overture (white arrowhead), circumperistomial kineties (white arrow), cilia of adoral membranelles (black arrow), and metachronal ciliary waves (asterisk). B, dorsal view showing the left protuberance (black arrowhead), metachronal ciliary waves (asterisk), the anterior margin of the peristomial overture (white arrowhead), and cilia of the circumperistomial kineties (black arrow).C, ventral view of showing characteristic lateral twisting of left protuberance also seen in vivo (black arrowhead, compare Fig.17E), the cytoproct (white arrowhead) and cilia of the adoral membranelles (black arrow). D, dorsal view showing straight course of the dorsal somatic kineties (white arrowhead), the left protuberance (white asterisk), cilia of the circumperistomial kineties (black arrowhead), and cilia of the adoral membranelles (black arrow). E, ventral view showing left-hand spiralling ventral somatic kineties (white asterisk). F, dorsal view showing straight dorsal somatic kineties (white arrowhead), cilia of circumperistomial kineties (black arrowhead), and area of somatic cilia matted during preparation (black asterisk). Scale bars:20 µm.	Figure 18. Clevelandella fryntai sp.nov.in the scanning electron microscope.From SRU. (A, B) slender morphotype, (C–F) broad morphotype. A, dorsal view showing cytoproct on left protuberance (black arrowhead), anterior margin of peristomial overture (white arrowhead), circumperistomial kineties (white arrow), cilia of adoral membranelles (black arrow), and metachronal ciliary waves (asterisk). B, dorsal view showing the left protuberance (black arrowhead), metachronal ciliary waves (asterisk), the anterior margin of the peristomial overture (white arrowhead), and cilia of the circumperistomial kineties (black arrow).C, ventral view of showing characteristic lateral twisting of left protuberance also seen in vivo (black arrowhead, compare Fig.17E), the cytoproct (white arrowhead) and cilia of the adoral membranelles (black arrow). D, dorsal view showing straight course of the dorsal somatic kineties (white arrowhead), the left protuberance (white asterisk), cilia of the circumperistomial kineties (black arrowhead), and cilia of the adoral membranelles (black arrow). E, ventral view showing left-hand spiralling ventral somatic kineties (white asterisk). F, dorsal view showing straight dorsal somatic kineties (white arrowhead), cilia of circumperistomial kineties (black arrowhead), and area of somatic cilia matted during preparation (black asterisk). Scale bars:20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191CFFA0FF1CFB649046F96C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542036/files/figure.png	https://doi.org/10.5281/zenodo.14542036	Figure 19.Anteclevelandella constricta in vivo (A–C) and after protargol impregnation (D–G). From PAA (A), STH population 1 (B) from STH population 2 (C), from ASS (D), from STH population 1 (E), and from SRU (F, G). A, left lateral view (optical section) showing two large contractile vacuole collecting vesicles (white arrowheads), adoral membranelles (black arrow) and left part of the karyophore (black arrowhead). B, ventral; view (optical section) showing contractile vacuole on the small left protuberance (white arrowhead). C, ventral view (optical section) showing shape variation with more narrowly tapered anterior end (white arrowheads), the micronucleus (white arrow), and the margin of the peristomial overture (black arrowhead). D, right lateral view showing kinetofragments in suture between ventral (white asterisk) and dorsal (black asterisk) somatic kineties, micronucleus (white arrowhead), paroral membrane (black arrow), and looping kineties on the peristomial projection (white arrow). E, ventral view (optical section) showing the transverse orientation of the macronucleus with right and left parts of the karyophore (black arrows) ans slightly pronounced left lobe (white arrow). F, ventral view showing position of the cytostome (white asterisk), paroral membrane (white arrowhead), and adoral membranelles (black arrowhead). G [same cell as (F)], detail view of the long (white arrowhead) and short (black arrowhead) files of the paroral membrane.Ma, macronucleus.Scale bars: 20 µm (A–F), 10 µm (G).	Figure 19.Anteclevelandella constricta in vivo (A–C) and after protargol impregnation (D–G). From PAA (A), STH population 1 (B) from STH population 2 (C), from ASS (D), from STH population 1 (E), and from SRU (F, G). A, left lateral view (optical section) showing two large contractile vacuole collecting vesicles (white arrowheads), adoral membranelles (black arrow) and left part of the karyophore (black arrowhead). B, ventral; view (optical section) showing contractile vacuole on the small left protuberance (white arrowhead). C, ventral view (optical section) showing shape variation with more narrowly tapered anterior end (white arrowheads), the micronucleus (white arrow), and the margin of the peristomial overture (black arrowhead). D, right lateral view showing kinetofragments in suture between ventral (white asterisk) and dorsal (black asterisk) somatic kineties, micronucleus (white arrowhead), paroral membrane (black arrow), and looping kineties on the peristomial projection (white arrow). E, ventral view (optical section) showing the transverse orientation of the macronucleus with right and left parts of the karyophore (black arrows) ans slightly pronounced left lobe (white arrow). F, ventral view showing position of the cytostome (white asterisk), paroral membrane (white arrowhead), and adoral membranelles (black arrowhead). G [same cell as (F)], detail view of the long (white arrowhead) and short (black arrowhead) files of the paroral membrane.Ma, macronucleus.Scale bars: 20 µm (A–F), 10 µm (G).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191CFFA0FF1CFB649046F96C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542038/files/figure.png	https://doi.org/10.5281/zenodo.14542038	Figure 20. Anteclevelandella constricta in the scanning electron microscope. From PAA. A, ventral view showing cilia of the adoral membranelles protruding from the peristomial overture (white arrowhead), the unciliated posterior body part (black asterisk), and cilia protruding from cytoproct on the left lateral protuberance (black arrowhead). B, dorsal view showing the right kinetal suture (black arrowhead) between the dorsal kineties (black asterisk) and ventral kineties (white asterisk) right suture, and sutural kinetofragments (black arrow). C, left ventrolateral view showing position of cytoproct on left posterior protuberance (black arrowhead) and the anterior margin of the peristomial overture (black arrow). D, left lateral view showing the location of the cytoproct (white arrowhead), short left kinetal suture (white arrow), and adoral membranells (black arrowhead). E, detail of same cell as (B) showing short transverse kinetofragments in anterior part of right suture (white arrows) between dorsal (black asterisk) and ventral (white asterisk) kineties. F, showing the leftward-spiralling ventral kineties which lack normal cilia (white arrows) and a cilium protruding from the cytoproct (black arrow). Scale bars: 20 µm (A–D), 5 µm (E–G).	Figure 20. Anteclevelandella constricta in the scanning electron microscope. From PAA. A, ventral view showing cilia of the adoral membranelles protruding from the peristomial overture (white arrowhead), the unciliated posterior body part (black asterisk), and cilia protruding from cytoproct on the left lateral protuberance (black arrowhead). B, dorsal view showing the right kinetal suture (black arrowhead) between the dorsal kineties (black asterisk) and ventral kineties (white asterisk) right suture, and sutural kinetofragments (black arrow). C, left ventrolateral view showing position of cytoproct on left posterior protuberance (black arrowhead) and the anterior margin of the peristomial overture (black arrow). D, left lateral view showing the location of the cytoproct (white arrowhead), short left kinetal suture (white arrow), and adoral membranells (black arrowhead). E, detail of same cell as (B) showing short transverse kinetofragments in anterior part of right suture (white arrows) between dorsal (black asterisk) and ventral (white asterisk) kineties. F, showing the leftward-spiralling ventral kineties which lack normal cilia (white arrows) and a cilium protruding from the cytoproct (black arrow). Scale bars: 20 µm (A–D), 5 µm (E–G).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191CFFA0FF1CFB649046F96C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542032/files/figure.png	https://doi.org/10.5281/zenodo.14542032	Figure 18. Clevelandella fryntai sp.nov.in the scanning electron microscope.From SRU. (A, B) slender morphotype, (C–F) broad morphotype. A, dorsal view showing cytoproct on left protuberance (black arrowhead), anterior margin of peristomial overture (white arrowhead), circumperistomial kineties (white arrow), cilia of adoral membranelles (black arrow), and metachronal ciliary waves (asterisk). B, dorsal view showing the left protuberance (black arrowhead), metachronal ciliary waves (asterisk), the anterior margin of the peristomial overture (white arrowhead), and cilia of the circumperistomial kineties (black arrow).C, ventral view of showing characteristic lateral twisting of left protuberance also seen in vivo (black arrowhead, compare Fig.17E), the cytoproct (white arrowhead) and cilia of the adoral membranelles (black arrow). D, dorsal view showing straight course of the dorsal somatic kineties (white arrowhead), the left protuberance (white asterisk), cilia of the circumperistomial kineties (black arrowhead), and cilia of the adoral membranelles (black arrow). E, ventral view showing left-hand spiralling ventral somatic kineties (white asterisk). F, dorsal view showing straight dorsal somatic kineties (white arrowhead), cilia of circumperistomial kineties (black arrowhead), and area of somatic cilia matted during preparation (black asterisk). Scale bars:20 µm.	Figure 18. Clevelandella fryntai sp.nov.in the scanning electron microscope.From SRU. (A, B) slender morphotype, (C–F) broad morphotype. A, dorsal view showing cytoproct on left protuberance (black arrowhead), anterior margin of peristomial overture (white arrowhead), circumperistomial kineties (white arrow), cilia of adoral membranelles (black arrow), and metachronal ciliary waves (asterisk). B, dorsal view showing the left protuberance (black arrowhead), metachronal ciliary waves (asterisk), the anterior margin of the peristomial overture (white arrowhead), and cilia of the circumperistomial kineties (black arrow).C, ventral view of showing characteristic lateral twisting of left protuberance also seen in vivo (black arrowhead, compare Fig.17E), the cytoproct (white arrowhead) and cilia of the adoral membranelles (black arrow). D, dorsal view showing straight course of the dorsal somatic kineties (white arrowhead), the left protuberance (white asterisk), cilia of the circumperistomial kineties (black arrowhead), and cilia of the adoral membranelles (black arrow). E, ventral view showing left-hand spiralling ventral somatic kineties (white asterisk). F, dorsal view showing straight dorsal somatic kineties (white arrowhead), cilia of circumperistomial kineties (black arrowhead), and area of somatic cilia matted during preparation (black asterisk). Scale bars:20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191CFFA1FCA6F9779781F879.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542042/files/figure.png	https://doi.org/10.5281/zenodo.14542042	Figure 21. Rhyncholevelandella sp.2 (A–C, G–I) from ASS. Rhynchoclevelandella nipponensis (D–F, J, K) from PAC.Rhynchoclevelandella hastula (L–T) from PGW. In vivo (A–F, L–O) and after protargol impregnation (G–K, P–T). A, ventral view (optical section) showing margin of the peristomial overture (black arrowhead) and adoral membranelles (black arrow). B, ventral view.C, ventral view showing cilia of circumperistomial kineties (white arrowhead). D, ventral view showing anterior margin of peristomial overture (black arrowhead). E, same cell showing ventral kineties (asterisk).F, ventral view showing contractile vacuole (white arrowhead), the relatively large micronucleus	Figure 21. Rhyncholevelandella sp.2 (A–C, G–I) from ASS. Rhynchoclevelandella nipponensis (D–F, J, K) from PAC.Rhynchoclevelandella hastula (L–T) from PGW. In vivo (A–F, L–O) and after protargol impregnation (G–K, P–T). A, ventral view (optical section) showing margin of the peristomial overture (black arrowhead) and adoral membranelles (black arrow). B, ventral view.C, ventral view showing cilia of circumperistomial kineties (white arrowhead). D, ventral view showing anterior margin of peristomial overture (black arrowhead). E, same cell showing ventral kineties (asterisk).F, ventral view showing contractile vacuole (white arrowhead), the relatively large micronucleus	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191AFFA6FF15F90B919EF80E.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542042/files/figure.png	https://doi.org/10.5281/zenodo.14542042	Figure 21. Rhyncholevelandella sp.2 (A–C, G–I) from ASS. Rhynchoclevelandella nipponensis (D–F, J, K) from PAC.Rhynchoclevelandella hastula (L–T) from PGW. In vivo (A–F, L–O) and after protargol impregnation (G–K, P–T). A, ventral view (optical section) showing margin of the peristomial overture (black arrowhead) and adoral membranelles (black arrow). B, ventral view.C, ventral view showing cilia of circumperistomial kineties (white arrowhead). D, ventral view showing anterior margin of peristomial overture (black arrowhead). E, same cell showing ventral kineties (asterisk).F, ventral view showing contractile vacuole (white arrowhead), the relatively large micronucleus	Figure 21. Rhyncholevelandella sp.2 (A–C, G–I) from ASS. Rhynchoclevelandella nipponensis (D–F, J, K) from PAC.Rhynchoclevelandella hastula (L–T) from PGW. In vivo (A–F, L–O) and after protargol impregnation (G–K, P–T). A, ventral view (optical section) showing margin of the peristomial overture (black arrowhead) and adoral membranelles (black arrow). B, ventral view.C, ventral view showing cilia of circumperistomial kineties (white arrowhead). D, ventral view showing anterior margin of peristomial overture (black arrowhead). E, same cell showing ventral kineties (asterisk).F, ventral view showing contractile vacuole (white arrowhead), the relatively large micronucleus	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191AFFA6FF15F90B919EF80E.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542046/files/figure.png	https://doi.org/10.5281/zenodo.14542046	Figure 22. Clevelandella hastula in the scanning electron microscope.From PGW.A, ventral view showing collapsed left lobar protrusion (black arrow), right margin of the peristomial overture (black arrowhead), left-hand spiralling unciliated part of ventral kineties (white arrowhead), and cilia of adoral membranelles (white arrow). B, dorsal view showing left lobar protrusion (black arrow) and unciliated parts of dorsal somatic kineties (black arrowhead) on the characteristically elongated peristomial protrusion (white arrow). C, left lateral view of posterior part of cell showing cilia protruding from the cytoproct on the dome of the left lobar protrusion (black arrow), the anterior margin of the peristomial overture (black arrowhead), cilia of the adoral membranelles (small black arrow), cilia of the circumperistomial kineties (white arrow), and the inconspicuous left posterior suture (white arrowhead) between dorsal kineties (black asterisk) and ventral kineties (white asterisk).Scale bars: 20 µm.	Figure 22. Clevelandella hastula in the scanning electron microscope.From PGW.A, ventral view showing collapsed left lobar protrusion (black arrow), right margin of the peristomial overture (black arrowhead), left-hand spiralling unciliated part of ventral kineties (white arrowhead), and cilia of adoral membranelles (white arrow). B, dorsal view showing left lobar protrusion (black arrow) and unciliated parts of dorsal somatic kineties (black arrowhead) on the characteristically elongated peristomial protrusion (white arrow). C, left lateral view of posterior part of cell showing cilia protruding from the cytoproct on the dome of the left lobar protrusion (black arrow), the anterior margin of the peristomial overture (black arrowhead), cilia of the adoral membranelles (small black arrow), cilia of the circumperistomial kineties (white arrow), and the inconspicuous left posterior suture (white arrowhead) between dorsal kineties (black asterisk) and ventral kineties (white asterisk).Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191AFFA6FEF5FA8395A1F9D0.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542042/files/figure.png	https://doi.org/10.5281/zenodo.14542042	Figure 21. Rhyncholevelandella sp.2 (A–C, G–I) from ASS. Rhynchoclevelandella nipponensis (D–F, J, K) from PAC.Rhynchoclevelandella hastula (L–T) from PGW. In vivo (A–F, L–O) and after protargol impregnation (G–K, P–T). A, ventral view (optical section) showing margin of the peristomial overture (black arrowhead) and adoral membranelles (black arrow). B, ventral view.C, ventral view showing cilia of circumperistomial kineties (white arrowhead). D, ventral view showing anterior margin of peristomial overture (black arrowhead). E, same cell showing ventral kineties (asterisk).F, ventral view showing contractile vacuole (white arrowhead), the relatively large micronucleus	Figure 21. Rhyncholevelandella sp.2 (A–C, G–I) from ASS. Rhynchoclevelandella nipponensis (D–F, J, K) from PAC.Rhynchoclevelandella hastula (L–T) from PGW. In vivo (A–F, L–O) and after protargol impregnation (G–K, P–T). A, ventral view (optical section) showing margin of the peristomial overture (black arrowhead) and adoral membranelles (black arrow). B, ventral view.C, ventral view showing cilia of circumperistomial kineties (white arrowhead). D, ventral view showing anterior margin of peristomial overture (black arrowhead). E, same cell showing ventral kineties (asterisk).F, ventral view showing contractile vacuole (white arrowhead), the relatively large micronucleus	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191BFFA5FC64F88B9075FA93.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542048/files/figure.png	https://doi.org/10.5281/zenodo.14542048	Figure 23. Paraclevelandia brevis, from life (A–C, H, I) and after protargol impregnation (D–G, J–P). Small ‘brevis’ morphotype (A–G), large ‘simplex’ morphotype (H–P); from ASS (A, H, I), SRU (J–M, K, O, P), STH (B, N), PAA (C), PGW (D–G). A, ventral view (optical section) showing the apical sac-like structure (black arrowhead) and adoral membranelles (white arrow). B, ventral view showing the subcortical layer of globules (probable mucocysts, black arrow), contractile vacuole (white arrowhead), adoral membranelles (white arrow), and circumperistomial cilia (black arrowhead). C, dorsal view showing relatively straight course of somatic kineties (asterisk). D, Ventral view (optical section) showing the micronucleus (white arrowhead) and adoral membranelles (white arrow). E, right ventrolateral view showing sutural kinetofragments (black arrowhead).F, right lateral view showing sutural kinetofragments (black arrowhead), ventral (white asterisk) and dorsal (black asterisk) kineties.G, detail view showing long (white arrowhead) and short (black arrowhead) files of the paroral membrane, asterisk indicates direction to anterior end of cell.H, ventral view (optical section) showing adoral membranelles (white arrow) and apical sac-like sructure (black arrowhead).I, ventral view showing left-hand spiralling somatic kineties (asterisk) and apical sac-like structure (black arrowhead). J–M, the same cell in ventral view: J, optical section showing inconspicuous anterior and posterior karyophore (white arrows), the separate apical sac-like structure (black arrowhead), and elliptical excretory antrum (white arrowheads); K, surface view showing sutural kinetofragmments (black arrowhead), left-hand spiralling ventral somatic kineties (asterisk), and peristomial overture (black arrow); L, excretory antrum (asterisk); M, elliptical cytoproct on left protuberance (black arrowhead). N, detail view showing long (white arrowhead) and short (black arrowhead) files of the diplostichomonad paroral membrane. Asterisk indicates anterior end. O, detail of macrunucleus and micronucleus (white arrowhead) and karyophore (black arrowhead). P, kinetofragments in right suture (black arrowheads).Ma, macronucleus. Scale bars:5 µm (A–F), 1.25 µm (G), 10 µm (H–K, O, P), 2 µm (L–N).	Figure 23. Paraclevelandia brevis, from life (A–C, H, I) and after protargol impregnation (D–G, J–P). Small ‘brevis’ morphotype (A–G), large ‘simplex’ morphotype (H–P); from ASS (A, H, I), SRU (J–M, K, O, P), STH (B, N), PAA (C), PGW (D–G). A, ventral view (optical section) showing the apical sac-like structure (black arrowhead) and adoral membranelles (white arrow). B, ventral view showing the subcortical layer of globules (probable mucocysts, black arrow), contractile vacuole (white arrowhead), adoral membranelles (white arrow), and circumperistomial cilia (black arrowhead). C, dorsal view showing relatively straight course of somatic kineties (asterisk). D, Ventral view (optical section) showing the micronucleus (white arrowhead) and adoral membranelles (white arrow). E, right ventrolateral view showing sutural kinetofragments (black arrowhead).F, right lateral view showing sutural kinetofragments (black arrowhead), ventral (white asterisk) and dorsal (black asterisk) kineties.G, detail view showing long (white arrowhead) and short (black arrowhead) files of the paroral membrane, asterisk indicates direction to anterior end of cell.H, ventral view (optical section) showing adoral membranelles (white arrow) and apical sac-like sructure (black arrowhead).I, ventral view showing left-hand spiralling somatic kineties (asterisk) and apical sac-like structure (black arrowhead). J–M, the same cell in ventral view: J, optical section showing inconspicuous anterior and posterior karyophore (white arrows), the separate apical sac-like structure (black arrowhead), and elliptical excretory antrum (white arrowheads); K, surface view showing sutural kinetofragmments (black arrowhead), left-hand spiralling ventral somatic kineties (asterisk), and peristomial overture (black arrow); L, excretory antrum (asterisk); M, elliptical cytoproct on left protuberance (black arrowhead). N, detail view showing long (white arrowhead) and short (black arrowhead) files of the diplostichomonad paroral membrane. Asterisk indicates anterior end. O, detail of macrunucleus and micronucleus (white arrowhead) and karyophore (black arrowhead). P, kinetofragments in right suture (black arrowheads).Ma, macronucleus. Scale bars:5 µm (A–F), 1.25 µm (G), 10 µm (H–K, O, P), 2 µm (L–N).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B3191BFFA5FC64F88B9075FA93.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542052/files/figure.png	https://doi.org/10.5281/zenodo.14542052	Figure 24. Paraclevelandia brevis in the scanning electron microscope. From PAC.Small ‘brevis’ morphotype (A, B, D, E), large ‘simplex’ morphotype (C, F). A, dorsal view of showing unciliated posterior parts of dorsal somatic kineties (white asterisk) and convergence of somatic kineties (whorl) on inconspicuous left posterior protuberance (black arrow).B, right ventrolateral view showing right sutural kinetofragments (white arrow) and margin of the peristomial overture (black arrow). C, detail view of right sutural kinetofragments (white arrow) between dorsal (black asterisk) and left-spiralling ventral (white asterisk) somatic kineties, from same cell as (F). D, detail view of same cell as (B)	Figure 24. Paraclevelandia brevis in the scanning electron microscope. From PAC.Small ‘brevis’ morphotype (A, B, D, E), large ‘simplex’ morphotype (C, F). A, dorsal view of showing unciliated posterior parts of dorsal somatic kineties (white asterisk) and convergence of somatic kineties (whorl) on inconspicuous left posterior protuberance (black arrow).B, right ventrolateral view showing right sutural kinetofragments (white arrow) and margin of the peristomial overture (black arrow). C, detail view of right sutural kinetofragments (white arrow) between dorsal (black asterisk) and left-spiralling ventral (white asterisk) somatic kineties, from same cell as (F). D, detail view of same cell as (B)	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31919FFA8FC6FFAE496CFFA19.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542048/files/figure.png	https://doi.org/10.5281/zenodo.14542048	Figure 23. Paraclevelandia brevis, from life (A–C, H, I) and after protargol impregnation (D–G, J–P). Small ‘brevis’ morphotype (A–G), large ‘simplex’ morphotype (H–P); from ASS (A, H, I), SRU (J–M, K, O, P), STH (B, N), PAA (C), PGW (D–G). A, ventral view (optical section) showing the apical sac-like structure (black arrowhead) and adoral membranelles (white arrow). B, ventral view showing the subcortical layer of globules (probable mucocysts, black arrow), contractile vacuole (white arrowhead), adoral membranelles (white arrow), and circumperistomial cilia (black arrowhead). C, dorsal view showing relatively straight course of somatic kineties (asterisk). D, Ventral view (optical section) showing the micronucleus (white arrowhead) and adoral membranelles (white arrow). E, right ventrolateral view showing sutural kinetofragments (black arrowhead).F, right lateral view showing sutural kinetofragments (black arrowhead), ventral (white asterisk) and dorsal (black asterisk) kineties.G, detail view showing long (white arrowhead) and short (black arrowhead) files of the paroral membrane, asterisk indicates direction to anterior end of cell.H, ventral view (optical section) showing adoral membranelles (white arrow) and apical sac-like sructure (black arrowhead).I, ventral view showing left-hand spiralling somatic kineties (asterisk) and apical sac-like structure (black arrowhead). J–M, the same cell in ventral view: J, optical section showing inconspicuous anterior and posterior karyophore (white arrows), the separate apical sac-like structure (black arrowhead), and elliptical excretory antrum (white arrowheads); K, surface view showing sutural kinetofragmments (black arrowhead), left-hand spiralling ventral somatic kineties (asterisk), and peristomial overture (black arrow); L, excretory antrum (asterisk); M, elliptical cytoproct on left protuberance (black arrowhead). N, detail view showing long (white arrowhead) and short (black arrowhead) files of the diplostichomonad paroral membrane. Asterisk indicates anterior end. O, detail of macrunucleus and micronucleus (white arrowhead) and karyophore (black arrowhead). P, kinetofragments in right suture (black arrowheads).Ma, macronucleus. Scale bars:5 µm (A–F), 1.25 µm (G), 10 µm (H–K, O, P), 2 µm (L–N).	Figure 23. Paraclevelandia brevis, from life (A–C, H, I) and after protargol impregnation (D–G, J–P). Small ‘brevis’ morphotype (A–G), large ‘simplex’ morphotype (H–P); from ASS (A, H, I), SRU (J–M, K, O, P), STH (B, N), PAA (C), PGW (D–G). A, ventral view (optical section) showing the apical sac-like structure (black arrowhead) and adoral membranelles (white arrow). B, ventral view showing the subcortical layer of globules (probable mucocysts, black arrow), contractile vacuole (white arrowhead), adoral membranelles (white arrow), and circumperistomial cilia (black arrowhead). C, dorsal view showing relatively straight course of somatic kineties (asterisk). D, Ventral view (optical section) showing the micronucleus (white arrowhead) and adoral membranelles (white arrow). E, right ventrolateral view showing sutural kinetofragments (black arrowhead).F, right lateral view showing sutural kinetofragments (black arrowhead), ventral (white asterisk) and dorsal (black asterisk) kineties.G, detail view showing long (white arrowhead) and short (black arrowhead) files of the paroral membrane, asterisk indicates direction to anterior end of cell.H, ventral view (optical section) showing adoral membranelles (white arrow) and apical sac-like sructure (black arrowhead).I, ventral view showing left-hand spiralling somatic kineties (asterisk) and apical sac-like structure (black arrowhead). J–M, the same cell in ventral view: J, optical section showing inconspicuous anterior and posterior karyophore (white arrows), the separate apical sac-like structure (black arrowhead), and elliptical excretory antrum (white arrowheads); K, surface view showing sutural kinetofragmments (black arrowhead), left-hand spiralling ventral somatic kineties (asterisk), and peristomial overture (black arrow); L, excretory antrum (asterisk); M, elliptical cytoproct on left protuberance (black arrowhead). N, detail view showing long (white arrowhead) and short (black arrowhead) files of the diplostichomonad paroral membrane. Asterisk indicates anterior end. O, detail of macrunucleus and micronucleus (white arrowhead) and karyophore (black arrowhead). P, kinetofragments in right suture (black arrowheads).Ma, macronucleus. Scale bars:5 µm (A–F), 1.25 µm (G), 10 µm (H–K, O, P), 2 µm (L–N).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31919FFA8FC6FFAE496CFFA19.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542052/files/figure.png	https://doi.org/10.5281/zenodo.14542052	Figure 24. Paraclevelandia brevis in the scanning electron microscope. From PAC.Small ‘brevis’ morphotype (A, B, D, E), large ‘simplex’ morphotype (C, F). A, dorsal view of showing unciliated posterior parts of dorsal somatic kineties (white asterisk) and convergence of somatic kineties (whorl) on inconspicuous left posterior protuberance (black arrow).B, right ventrolateral view showing right sutural kinetofragments (white arrow) and margin of the peristomial overture (black arrow). C, detail view of right sutural kinetofragments (white arrow) between dorsal (black asterisk) and left-spiralling ventral (white asterisk) somatic kineties, from same cell as (F). D, detail view of same cell as (B)	Figure 24. Paraclevelandia brevis in the scanning electron microscope. From PAC.Small ‘brevis’ morphotype (A, B, D, E), large ‘simplex’ morphotype (C, F). A, dorsal view of showing unciliated posterior parts of dorsal somatic kineties (white asterisk) and convergence of somatic kineties (whorl) on inconspicuous left posterior protuberance (black arrow).B, right ventrolateral view showing right sutural kinetofragments (white arrow) and margin of the peristomial overture (black arrow). C, detail view of right sutural kinetofragments (white arrow) between dorsal (black asterisk) and left-spiralling ventral (white asterisk) somatic kineties, from same cell as (F). D, detail view of same cell as (B)	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31914FFAFFEC8FA579690FD84.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542054/files/figure.png	https://doi.org/10.5281/zenodo.14542054	Figure 25. Clevelandella elongata in vivo. From SRU.A, ventral view showing adoral membranelles (black arrow), cytostome (white arrow), contractile vacuole (white arrowhead), and hyaline subcortical layer (black arrowheads). B, left ventrolateral view (optical section) showing micronucleus (white arrow), karyophore (black arrows), contractile vacuole (white arrowhead), and posterior extent of ciliation (black arrowheads). C, left ventrolateral view showing micronucleus (white arrow), karyophore (black arrow), and cilia of the circumperistomial kineties (white arrowheads). D, detail of same cell as (C) showing peristomial overture (black arrowhead) and cilia of adoral membranelles (white arrow). Ma, macronucleus. Scale 20 µm.	Figure 25. Clevelandella elongata in vivo. From SRU.A, ventral view showing adoral membranelles (black arrow), cytostome (white arrow), contractile vacuole (white arrowhead), and hyaline subcortical layer (black arrowheads). B, left ventrolateral view (optical section) showing micronucleus (white arrow), karyophore (black arrows), contractile vacuole (white arrowhead), and posterior extent of ciliation (black arrowheads). C, left ventrolateral view showing micronucleus (white arrow), karyophore (black arrow), and cilia of the circumperistomial kineties (white arrowheads). D, detail of same cell as (C) showing peristomial overture (black arrowhead) and cilia of adoral membranelles (white arrow). Ma, macronucleus. Scale 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31914FFAFFEC8FA579690FD84.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542058/files/figure.png	https://doi.org/10.5281/zenodo.14542058	Figure 26. Clevelandella elongata after protargol impregnation. From SRU. A, ventral view (optical section) showing micronucleus (white arrow), anterior part of the karyophore (black arrow), and paroral membrane (black arrowhead).B–E, the same cell in ventral view: B, (optical section) showing anterior part of karyophore (black arrow), micronucleus (white arrowhead), adoral membranelles (white arrow), and paroral membrane (black arrowhead); C, ventral infraciliature showing right kinetal suture (black arrowhead), left-hand spiralling ventral kineties (asterisk), and kineties converging to form ciliary whorl on inconspicuous left protuberance (white arrowhead); D, dorsal infraciliature showing straight course of dorsal kineties (asterisk) and looping kineties of peristomial projection (black arrowhead); E, detail view of the long file (black arrowhead) and short file (white arrowhead) of the paroral membrane. F, detail showing anterior (black arrowhead) and posterior (white arrowhead) parts of the karyophore and the micronucleus (white arrow). Ma, macronucleus. Scale bars: 20 µm.	Figure 26. Clevelandella elongata after protargol impregnation. From SRU. A, ventral view (optical section) showing micronucleus (white arrow), anterior part of the karyophore (black arrow), and paroral membrane (black arrowhead).B–E, the same cell in ventral view: B, (optical section) showing anterior part of karyophore (black arrow), micronucleus (white arrowhead), adoral membranelles (white arrow), and paroral membrane (black arrowhead); C, ventral infraciliature showing right kinetal suture (black arrowhead), left-hand spiralling ventral kineties (asterisk), and kineties converging to form ciliary whorl on inconspicuous left protuberance (white arrowhead); D, dorsal infraciliature showing straight course of dorsal kineties (asterisk) and looping kineties of peristomial projection (black arrowhead); E, detail view of the long file (black arrowhead) and short file (white arrowhead) of the paroral membrane. F, detail showing anterior (black arrowhead) and posterior (white arrowhead) parts of the karyophore and the micronucleus (white arrow). Ma, macronucleus. Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31914FFAFFEC8FA579690FD84.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542060/files/figure.png	https://doi.org/10.5281/zenodo.14542060	Figure 27. Clevelandella elongata in the scanning electron microscope. From SRU.A, right ventrolateral view showing metachronal ciliary waves (white arrows), and ‘tail’ of ciliated basal bodies extending posteriorly on right ventrolateral surface (white arrowhead) near the right suture (white asterisk). Cilia of the adoral membranelles protrude from the buccal overture (black arrow). Cilia of the collecting chamber protrude from the cytoproct (black arrowhead). B, dorsal view showing ‘tail’ of ciliated basal bodies extending posteriorly on right ventrolateral surface (black arrowhead) and cilia of circumperistomial kineties (white arrowhead). C, left dorsolateral view showing the cytoproct (black arrowhead), dorsal kineties (black asterisk), proximal margin of buccal overture (white arrow), cilia of the adoral membranelles (black arrow), and circumperistomial kineties (white arrowhead). D, right dorsolateral view showing posteriorly extending ‘tail’ of cilia (black arrowhead), right suture between dorsal kineties (white asterisk) and ventral kineties (black asterisk), and densely ciliated circumperistomial kineties (white arrow). E, dorsal view showing straight dorsal kineties (white asterisk), the ciliary ‘tail’ (black arrowhead), and cilia of circumperistomial kineties (white arrow). F, posterodorsal view showing convexity of the right dorsolateral cell surface (white asterisk), cilia of circumperistomial kineties (white arrowhead), and cilia of adoral membranelles (black arrow). G, left lateral detail view showing proximal margin of buccal overture (white arrow), circumperistomial cilia (black arrow), adoral membranelles (black arrowheads), and paroral membrane (white arrowheads).H, posterolateral detail view of the buccal overture showing posteriormost adoral membranelle (white arrow), circumperistomial cilia (black arrow), right suture (white arrowhead) between ventral kineties (black asterisk) and dorsal kineties (white asterisk). I, posterior view of buccal overture showing adoral membranelles (black arrowhead), paroral membrane (white arrowhead), and circumperistomial cilia (white arrow). Scale bars: 50 µm (A–E), 20 µm (F–I).	Figure 27. Clevelandella elongata in the scanning electron microscope. From SRU.A, right ventrolateral view showing metachronal ciliary waves (white arrows), and ‘tail’ of ciliated basal bodies extending posteriorly on right ventrolateral surface (white arrowhead) near the right suture (white asterisk). Cilia of the adoral membranelles protrude from the buccal overture (black arrow). Cilia of the collecting chamber protrude from the cytoproct (black arrowhead). B, dorsal view showing ‘tail’ of ciliated basal bodies extending posteriorly on right ventrolateral surface (black arrowhead) and cilia of circumperistomial kineties (white arrowhead). C, left dorsolateral view showing the cytoproct (black arrowhead), dorsal kineties (black asterisk), proximal margin of buccal overture (white arrow), cilia of the adoral membranelles (black arrow), and circumperistomial kineties (white arrowhead). D, right dorsolateral view showing posteriorly extending ‘tail’ of cilia (black arrowhead), right suture between dorsal kineties (white asterisk) and ventral kineties (black asterisk), and densely ciliated circumperistomial kineties (white arrow). E, dorsal view showing straight dorsal kineties (white asterisk), the ciliary ‘tail’ (black arrowhead), and cilia of circumperistomial kineties (white arrow). F, posterodorsal view showing convexity of the right dorsolateral cell surface (white asterisk), cilia of circumperistomial kineties (white arrowhead), and cilia of adoral membranelles (black arrow). G, left lateral detail view showing proximal margin of buccal overture (white arrow), circumperistomial cilia (black arrow), adoral membranelles (black arrowheads), and paroral membrane (white arrowheads).H, posterolateral detail view of the buccal overture showing posteriormost adoral membranelle (white arrow), circumperistomial cilia (black arrow), right suture (white arrowhead) between ventral kineties (black asterisk) and dorsal kineties (white asterisk). I, posterior view of buccal overture showing adoral membranelles (black arrowhead), paroral membrane (white arrowhead), and circumperistomial cilia (white arrow). Scale bars: 50 µm (A–E), 20 µm (F–I).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFF1BFA3491A0FD81.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542068/files/figure.png	https://doi.org/10.5281/zenodo.14542068	Figure 30. Clevelandella kidderi in vivo (A, B) and after protargol impregnation (C–E). From ASS (A–E). A, ventral view showing contractile vacuole (white arrow), ventral kineties (black arrow), and margin of peristomial overture (white arrowhead). B, dorsal view showing contractile vacuole (white arrow) and posterior extent of ciliation on the left margin (black arrowhead). C, ventral infraciliature showing right sutural kinetofragments (black arrow), left-hand spiralling ventral kineties (asterisk), and looping kineties of the peristomial projection (white arrowhead). D, detail view of paroral membrane showing short (white arrowhead) and long (black arrowhead) files, and adoral membranelles, (white arrow). E, detail of macronucleus, ventral view showing inconspicuous karyophore (black arrow). Ma, macronucleus. Scale bars: 20 µm (A–C), 10 µm (D).	Figure 30. Clevelandella kidderi in vivo (A, B) and after protargol impregnation (C–E). From ASS (A–E). A, ventral view showing contractile vacuole (white arrow), ventral kineties (black arrow), and margin of peristomial overture (white arrowhead). B, dorsal view showing contractile vacuole (white arrow) and posterior extent of ciliation on the left margin (black arrowhead). C, ventral infraciliature showing right sutural kinetofragments (black arrow), left-hand spiralling ventral kineties (asterisk), and looping kineties of the peristomial projection (white arrowhead). D, detail view of paroral membrane showing short (white arrowhead) and long (black arrowhead) files, and adoral membranelles, (white arrow). E, detail of macronucleus, ventral view showing inconspicuous karyophore (black arrow). Ma, macronucleus. Scale bars: 20 µm (A–C), 10 µm (D).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFF1BFA3491A0FD81.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542072/files/figure.png	https://doi.org/10.5281/zenodo.14542072	Figure 31. Clevelandella kidderi in the scanning electron microscope (A–D). From ASS. A, ventral view showing left-hand-spiralling ventral kineties (black asterisk) meeting dorsal kineties (white asterisk) to form right kinetal suture (white arrowhead), cilia protruding from cytoproct (black arrowhead), and ciliated circumperistomial kineties (black arrow). B, left lateral view showing unciliated parts of dorsal kineties (black asterisk) and ventral kineties (white asterisk), cilia protruding from cytoproct (black arrowhead), and cilia of adoral membranelles seen in peristomial overture (white arrow). C, posterior view showing dorsoventral flattening and right kinetal suture (white arrowhead) between ventral kineties (black asterisk) and dorsal kineties (white asterisk), and dorsal margin of the posterior peristomial projection (white arrow). D, detail of the same cell as (B) showing cilia protruding from cytoproct (black arrowhead) on the left protuberance (asterisk), and posterior extent of somatic ciliation (white arrow). Scale bars: 20 µm (A–C), 10 µm (D).	Figure 31. Clevelandella kidderi in the scanning electron microscope (A–D). From ASS. A, ventral view showing left-hand-spiralling ventral kineties (black asterisk) meeting dorsal kineties (white asterisk) to form right kinetal suture (white arrowhead), cilia protruding from cytoproct (black arrowhead), and ciliated circumperistomial kineties (black arrow). B, left lateral view showing unciliated parts of dorsal kineties (black asterisk) and ventral kineties (white asterisk), cilia protruding from cytoproct (black arrowhead), and cilia of adoral membranelles seen in peristomial overture (white arrow). C, posterior view showing dorsoventral flattening and right kinetal suture (white arrowhead) between ventral kineties (black asterisk) and dorsal kineties (white asterisk), and dorsal margin of the posterior peristomial projection (white arrow). D, detail of the same cell as (B) showing cilia protruding from cytoproct (black arrowhead) on the left protuberance (asterisk), and posterior extent of somatic ciliation (white arrow). Scale bars: 20 µm (A–C), 10 µm (D).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFC6BFDD890F8FAE7.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542074/files/figure.png	https://doi.org/10.5281/zenodo.14542074	Figure 32. Clevelandella panesthiae in vivo (A–C) and after protargol impregnation (D, E). From ASS (A), PAA (B, D, E), and PT (C). A, ventral view (optical section) of typical form showing the contractile vacuole (white arrow), cytoplasmic granules, possibly amylopectin (black arrow), circumperistomial kineties (black arrow), and hyaline subcortical layer (white arrowheads).B, ventral view (optical section) showing location of the cytostome (asterisk), adoral membranelles, (white arrow), and cytoplasmic granules, possibly amylopectin (black arrow). C, ventral view (optical section) showing posterior extent of ciliation on the right margin and left margin (black arrowheads), and unusually tapered anterior end of cell (white arrow heads).D, E, the same cell in ventral view:D, optical section showing cytostome (white arrowhead) amd adoral membranells (white arrow); E, surface view showing ventral infraciliature showing left-hand spiralling ventral kineties (asterisk), disordered basal bodies on left protuberance (black arrowhead). Ma, macronucleus.Scale bars: 20 µm.	Figure 32. Clevelandella panesthiae in vivo (A–C) and after protargol impregnation (D, E). From ASS (A), PAA (B, D, E), and PT (C). A, ventral view (optical section) of typical form showing the contractile vacuole (white arrow), cytoplasmic granules, possibly amylopectin (black arrow), circumperistomial kineties (black arrow), and hyaline subcortical layer (white arrowheads).B, ventral view (optical section) showing location of the cytostome (asterisk), adoral membranelles, (white arrow), and cytoplasmic granules, possibly amylopectin (black arrow). C, ventral view (optical section) showing posterior extent of ciliation on the right margin and left margin (black arrowheads), and unusually tapered anterior end of cell (white arrow heads).D, E, the same cell in ventral view:D, optical section showing cytostome (white arrowhead) amd adoral membranells (white arrow); E, surface view showing ventral infraciliature showing left-hand spiralling ventral kineties (asterisk), disordered basal bodies on left protuberance (black arrowhead). Ma, macronucleus.Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFC6BFDD890F8FAE7.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542078/files/figure.png	https://doi.org/10.5281/zenodo.14542078	Figure 33. Clevelandella panesthiae in vivo (optical sections of same cell). From ASS.A, surface view showing left-hand spiralling ventral kineties (asterisk), right kinetal suture (black arrowhead), and position of the cytoproct (white arrow). B, slightly deeper focal plane showing conical ciliated excretory antrum within left protuberance (white arrowheads) and defecated material (black arrowhead). C, slightly deeper focal plane than (B) showing faecal material transiting the ciliated excretory antrum (white arrowhead) and numerous rod-shaped perinuclear symbionts (black arrow and inset). D, approximately same focal plane as (C) showing collecting vesicles of contractile vacuole (white arrowhead) which will coalesce and empty into the excretory antrum and the dense, fine cilia lining the antrum (black arrowhead). Ma, macronucleus. Scale bars: 20 µm.	Figure 33. Clevelandella panesthiae in vivo (optical sections of same cell). From ASS.A, surface view showing left-hand spiralling ventral kineties (asterisk), right kinetal suture (black arrowhead), and position of the cytoproct (white arrow). B, slightly deeper focal plane showing conical ciliated excretory antrum within left protuberance (white arrowheads) and defecated material (black arrowhead). C, slightly deeper focal plane than (B) showing faecal material transiting the ciliated excretory antrum (white arrowhead) and numerous rod-shaped perinuclear symbionts (black arrow and inset). D, approximately same focal plane as (C) showing collecting vesicles of contractile vacuole (white arrowhead) which will coalesce and empty into the excretory antrum and the dense, fine cilia lining the antrum (black arrowhead). Ma, macronucleus. Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFC6BFDD890F8FAE7.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542080/files/figure.png	https://doi.org/10.5281/zenodo.14542080	Figure 34. Clevelandella panesthiae in the scanning electron microscope. From ASS. A, ventral view showing right kinetal suture (white arrow) and short left suture (white arrowhead), cilia of adoral membranelles (black arrowhead), and ciliated cytoproct (black arrow).	Figure 34. Clevelandella panesthiae in the scanning electron microscope. From ASS. A, ventral view showing right kinetal suture (white arrow) and short left suture (white arrowhead), cilia of adoral membranelles (black arrowhead), and ciliated cytoproct (black arrow).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFF1BFDDF96BFFA2D.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542062/files/figure.png	https://doi.org/10.5281/zenodo.14542062	Figure 28. Clevelandella parapanesthiae. In vivo (A, B) and after protargol impregnation (C–H). From PGW (A), MP (B, F), ASS (C, D), PT (E), and PGW (G, H). A, ventral view showing adoral membranelles (white arrow), contractile vacuole (white arrowhead), and micronucleus (black arrowhead). B, ventral view showing atypical macronuclear ‘S’-shape and adoral membranelles (white arrow). C, dorsal view (optical section) showing straight dorsal kineties (black arrowhead), adoral membranelles (white arrow), and left protuberance (asterisk). D, right lateral detail view of sutural kinetofragments (black arrowhead) and dorsal kinety (white arrowhead). E, ventral view (optical section) showing adoral membranelles (white arrow) and left protuberance (asterisk).F, detail view of paroral membrane showing short (white arrowhead) and long (black arrowhead) files.G, dorsal view showing straight dorsal kineties (black arrowhead). H, ventral view showing paroral membrane (black arrow head), prominent notch (white arrowhead)	Figure 28. Clevelandella parapanesthiae. In vivo (A, B) and after protargol impregnation (C–H). From PGW (A), MP (B, F), ASS (C, D), PT (E), and PGW (G, H). A, ventral view showing adoral membranelles (white arrow), contractile vacuole (white arrowhead), and micronucleus (black arrowhead). B, ventral view showing atypical macronuclear ‘S’-shape and adoral membranelles (white arrow). C, dorsal view (optical section) showing straight dorsal kineties (black arrowhead), adoral membranelles (white arrow), and left protuberance (asterisk). D, right lateral detail view of sutural kinetofragments (black arrowhead) and dorsal kinety (white arrowhead). E, ventral view (optical section) showing adoral membranelles (white arrow) and left protuberance (asterisk).F, detail view of paroral membrane showing short (white arrowhead) and long (black arrowhead) files.G, dorsal view showing straight dorsal kineties (black arrowhead). H, ventral view showing paroral membrane (black arrow head), prominent notch (white arrowhead)	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FFAFFF1BFDDF96BFFA2D.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542064/files/figure.png	https://doi.org/10.5281/zenodo.14542064	Figure 29. Clevelandella parapanesthiae in the scanning electron microscope. From PAC. A, ventral view showing notch (white arrow) between left protuberance (white asterisk) and peristomial projection (black asterisk), cilia protruding from cytoproct (black arrow), and cilia of adoral membranelles (black arrowhead). B, dorsal view showing notch (white arrow) between left protuberance (white asterisk) and peristomial projection (black asterisk), and cilia of circumperistomial kineties (black arrow). C, posteroventral view showing posterior protuberance (white asterisk), cilia of adoral membranelles (white arrow), and cilia of circumperistomial kineties (black arrow). D, posterior view showing dorsoventral flattening, right kinetal suture (black arrow) between ventral kineties (white asterisk) and dorsal kineties (black asterisk), position of cytoproct (white arrow), and peristomial overture (white arrowhead). Scale bars: 20 µm (A–D).	Figure 29. Clevelandella parapanesthiae in the scanning electron microscope. From PAC. A, ventral view showing notch (white arrow) between left protuberance (white asterisk) and peristomial projection (black asterisk), cilia protruding from cytoproct (black arrow), and cilia of adoral membranelles (black arrowhead). B, dorsal view showing notch (white arrow) between left protuberance (white asterisk) and peristomial projection (black asterisk), and cilia of circumperistomial kineties (black arrow). C, posteroventral view showing posterior protuberance (white asterisk), cilia of adoral membranelles (white arrow), and cilia of circumperistomial kineties (black arrow). D, posterior view showing dorsoventral flattening, right kinetal suture (black arrow) between ventral kineties (white asterisk) and dorsal kineties (black asterisk), position of cytoproct (white arrow), and peristomial overture (white arrowhead). Scale bars: 20 µm (A–D).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FF93FBD8FAF897C3FEFB.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542048/files/figure.png	https://doi.org/10.5281/zenodo.14542048	Figure 23. Paraclevelandia brevis, from life (A–C, H, I) and after protargol impregnation (D–G, J–P). Small ‘brevis’ morphotype (A–G), large ‘simplex’ morphotype (H–P); from ASS (A, H, I), SRU (J–M, K, O, P), STH (B, N), PAA (C), PGW (D–G). A, ventral view (optical section) showing the apical sac-like structure (black arrowhead) and adoral membranelles (white arrow). B, ventral view showing the subcortical layer of globules (probable mucocysts, black arrow), contractile vacuole (white arrowhead), adoral membranelles (white arrow), and circumperistomial cilia (black arrowhead). C, dorsal view showing relatively straight course of somatic kineties (asterisk). D, Ventral view (optical section) showing the micronucleus (white arrowhead) and adoral membranelles (white arrow). E, right ventrolateral view showing sutural kinetofragments (black arrowhead).F, right lateral view showing sutural kinetofragments (black arrowhead), ventral (white asterisk) and dorsal (black asterisk) kineties.G, detail view showing long (white arrowhead) and short (black arrowhead) files of the paroral membrane, asterisk indicates direction to anterior end of cell.H, ventral view (optical section) showing adoral membranelles (white arrow) and apical sac-like sructure (black arrowhead).I, ventral view showing left-hand spiralling somatic kineties (asterisk) and apical sac-like structure (black arrowhead). J–M, the same cell in ventral view: J, optical section showing inconspicuous anterior and posterior karyophore (white arrows), the separate apical sac-like structure (black arrowhead), and elliptical excretory antrum (white arrowheads); K, surface view showing sutural kinetofragmments (black arrowhead), left-hand spiralling ventral somatic kineties (asterisk), and peristomial overture (black arrow); L, excretory antrum (asterisk); M, elliptical cytoproct on left protuberance (black arrowhead). N, detail view showing long (white arrowhead) and short (black arrowhead) files of the diplostichomonad paroral membrane. Asterisk indicates anterior end. O, detail of macrunucleus and micronucleus (white arrowhead) and karyophore (black arrowhead). P, kinetofragments in right suture (black arrowheads).Ma, macronucleus. Scale bars:5 µm (A–F), 1.25 µm (G), 10 µm (H–K, O, P), 2 µm (L–N).	Figure 23. Paraclevelandia brevis, from life (A–C, H, I) and after protargol impregnation (D–G, J–P). Small ‘brevis’ morphotype (A–G), large ‘simplex’ morphotype (H–P); from ASS (A, H, I), SRU (J–M, K, O, P), STH (B, N), PAA (C), PGW (D–G). A, ventral view (optical section) showing the apical sac-like structure (black arrowhead) and adoral membranelles (white arrow). B, ventral view showing the subcortical layer of globules (probable mucocysts, black arrow), contractile vacuole (white arrowhead), adoral membranelles (white arrow), and circumperistomial cilia (black arrowhead). C, dorsal view showing relatively straight course of somatic kineties (asterisk). D, Ventral view (optical section) showing the micronucleus (white arrowhead) and adoral membranelles (white arrow). E, right ventrolateral view showing sutural kinetofragments (black arrowhead).F, right lateral view showing sutural kinetofragments (black arrowhead), ventral (white asterisk) and dorsal (black asterisk) kineties.G, detail view showing long (white arrowhead) and short (black arrowhead) files of the paroral membrane, asterisk indicates direction to anterior end of cell.H, ventral view (optical section) showing adoral membranelles (white arrow) and apical sac-like sructure (black arrowhead).I, ventral view showing left-hand spiralling somatic kineties (asterisk) and apical sac-like structure (black arrowhead). J–M, the same cell in ventral view: J, optical section showing inconspicuous anterior and posterior karyophore (white arrows), the separate apical sac-like structure (black arrowhead), and elliptical excretory antrum (white arrowheads); K, surface view showing sutural kinetofragmments (black arrowhead), left-hand spiralling ventral somatic kineties (asterisk), and peristomial overture (black arrow); L, excretory antrum (asterisk); M, elliptical cytoproct on left protuberance (black arrowhead). N, detail view showing long (white arrowhead) and short (black arrowhead) files of the diplostichomonad paroral membrane. Asterisk indicates anterior end. O, detail of macrunucleus and micronucleus (white arrowhead) and karyophore (black arrowhead). P, kinetofragments in right suture (black arrowheads).Ma, macronucleus. Scale bars:5 µm (A–F), 1.25 µm (G), 10 µm (H–K, O, P), 2 µm (L–N).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FF93FBD8FAF897C3FEFB.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542082/files/figure.png	https://doi.org/10.5281/zenodo.14542082	Figure 35. Non-metric multidimensional scaling (NMDS) plot based on the morphological analysis of traits measured on 455 scales. The cells are colour-coded and clustered based on their affiliation to 16 investigated strains.	Figure 35. Non-metric multidimensional scaling (NMDS) plot based on the morphological analysis of traits measured on 455 scales. The cells are colour-coded and clustered based on their affiliation to 16 investigated strains.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FF93FBD8FAF897C3FEFB.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541997/files/figure.png	https://doi.org/10.5281/zenodo.14541997	Figure 6. Schematic drawings of Clevelandella philipi sp. nov. from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view showing the oral apparatus and nuclear structures. C, ventral infraciliature.D, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties. Scale bars:20 µm.	Figure 6. Schematic drawings of Clevelandella philipi sp. nov. from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view showing the oral apparatus and nuclear structures. C, ventral infraciliature.D, dorsal infraciliature.AZM, adoral zone of membranelles; CPK, cilia of circumperistomial kineties; CV, contractile vacuole; HL, hyaline subcortical layer; Ma, macronucleus; Mi, micronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties. Scale bars:20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31913FF93FBD8FAF897C3FEFB.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541999/files/figure.png	https://doi.org/10.5281/zenodo.14541999	Figure 7. Clevelandella philipi sp. nov., in vivo (A, B) and after protargol impregnation (C–I). From STH (A–G) and ASS (H, I). A, ventral view showing thick, hyaline cortical layer (between black arrowheads), cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B, ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A, B). C, ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D, dorsal view showing infraciliature, ciliated circumperistomial kineties (black arrow), looping kineties of peristomial projection (white arrow), and cytoproct (black arrowhead).E, ventral view showing left-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F, dorsal view showing straight somatic kineties (white asterisk), disordered basal bodies on left protuberance (white arrow), and paroral membrane (black arrowhead). G, dorsal view showing micronucleus (white arrowhead). H, dorsal view showing straight dorsal somatic kineties (asterisk), proximal margin of the peristomial overture (white arrow), and looping kineties of peristomial projection (black arrowhead). I, detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer left file (white arrowhead) of basal bodies. Ma, macronucleus. Scale bars:20 µm (A–H) 10 µm (I).	Figure 7. Clevelandella philipi sp. nov., in vivo (A, B) and after protargol impregnation (C–I). From STH (A–G) and ASS (H, I). A, ventral view showing thick, hyaline cortical layer (between black arrowheads), cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B, ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A, B). C, ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D, dorsal view showing infraciliature, ciliated circumperistomial kineties (black arrow), looping kineties of peristomial projection (white arrow), and cytoproct (black arrowhead).E, ventral view showing left-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F, dorsal view showing straight somatic kineties (white asterisk), disordered basal bodies on left protuberance (white arrow), and paroral membrane (black arrowhead). G, dorsal view showing micronucleus (white arrowhead). H, dorsal view showing straight dorsal somatic kineties (asterisk), proximal margin of the peristomial overture (white arrow), and looping kineties of peristomial projection (black arrowhead). I, detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer left file (white arrowhead) of basal bodies. Ma, macronucleus. Scale bars:20 µm (A–H) 10 µm (I).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31920FF9DFC1CF932962FFE4E.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541993/files/figure.png	https://doi.org/10.5281/zenodo.14541993	Figure 4. Clevelandella ananiasi sp. nov. from ASS. In vivo (A, B) and after protargol impregnation (C–G). A, ventral view showing karyophore (white arrowhead), contractile vacuole (asterisk), posterior extent of somatic cilia (black arrowhead), and cilia of circumperistomial kineties (black arrow). B, ventral view showing a more slender macronucleus, karyophore (white arrowhead), contractile vacuole (asterisk), and adoral membranelles (black arrowhead).C, ventral view showing excretory (black arrow). D, ventral infraciliature.E, dorsal infraciliature of same cell as (D). F, detail view of peristomial projection showing the peristomial overture (black arrowhead) and cilia of the circumperistomial kineties (white arrowheads).G, detail view showing position of micronucleus (white arrow). Ma, macronucleus. Scale bars:20 µm (A–C), 10 µm (D–F), 5 µm (G).	Figure 4. Clevelandella ananiasi sp. nov. from ASS. In vivo (A, B) and after protargol impregnation (C–G). A, ventral view showing karyophore (white arrowhead), contractile vacuole (asterisk), posterior extent of somatic cilia (black arrowhead), and cilia of circumperistomial kineties (black arrow). B, ventral view showing a more slender macronucleus, karyophore (white arrowhead), contractile vacuole (asterisk), and adoral membranelles (black arrowhead).C, ventral view showing excretory (black arrow). D, ventral infraciliature.E, dorsal infraciliature of same cell as (D). F, detail view of peristomial projection showing the peristomial overture (black arrowhead) and cilia of the circumperistomial kineties (white arrowheads).G, detail view showing position of micronucleus (white arrow). Ma, macronucleus. Scale bars:20 µm (A–C), 10 µm (D–F), 5 µm (G).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31921FF9DFEECFD96966BFB1F.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542030/files/figure.png	https://doi.org/10.5281/zenodo.14542030	Figure 17.Clevelandella fryntai sp.nov.from life (A–E) and after protargol impregnation (F–K).From SRU.(A, B, F, G, K) slender morphotype, (C–E, H–J) broad morphotype.A, ventral view showing posterior extent of ciliation on the right margin (white arrowhead) and left margin (black arrowhead).B, dorsal view showing contractile vacuole (white arrowhead) and straight dorsal kineties (white arrow).C, ventral view (optical section) showing cytoplasmic granules, possibly amylopectin (black arrow), right kinetal suture (white arrowhead), adoral membranelles (black arrowhead),and cytoproct (white arrow).D, ventral view showing left-hand spiralling kineties (white arrowhead) and cilia of adoral membranelles (white arrow).E, ventral view showing the margin of the peristomial overture (black arrowhead) and bulbous left protuberance extending over left cell margin (white arrowhead).F, detail showing karyophore(black arrowhead).G, ventral view (optical section) showing the karyophore (black arrowhead), micronucleus (white arrow), and cytoproct (white arrowhead).H, dorsal view showing karyophore (black arrowheads), micronucleus (white arrowhead), and adoral membranelles (black arrow).I, ventral infraciliature showing left-hand spiralling kineties (asterisk) and looping kineties of the peristomial projection (white arrow). J, dorsal infraciliature showing straight dorsal kineties (asterisk). K, detail view showing diplostichomonad paroral (black arrow).Ma, macronucleus.Scale bars: 20 µm (A–E, G–J), 10 µm (F, K).	Figure 17.Clevelandella fryntai sp.nov.from life (A–E) and after protargol impregnation (F–K).From SRU.(A, B, F, G, K) slender morphotype, (C–E, H–J) broad morphotype.A, ventral view showing posterior extent of ciliation on the right margin (white arrowhead) and left margin (black arrowhead).B, dorsal view showing contractile vacuole (white arrowhead) and straight dorsal kineties (white arrow).C, ventral view (optical section) showing cytoplasmic granules, possibly amylopectin (black arrow), right kinetal suture (white arrowhead), adoral membranelles (black arrowhead),and cytoproct (white arrow).D, ventral view showing left-hand spiralling kineties (white arrowhead) and cilia of adoral membranelles (white arrow).E, ventral view showing the margin of the peristomial overture (black arrowhead) and bulbous left protuberance extending over left cell margin (white arrowhead).F, detail showing karyophore(black arrowhead).G, ventral view (optical section) showing the karyophore (black arrowhead), micronucleus (white arrow), and cytoproct (white arrowhead).H, dorsal view showing karyophore (black arrowheads), micronucleus (white arrowhead), and adoral membranelles (black arrow).I, ventral infraciliature showing left-hand spiralling kineties (asterisk) and looping kineties of the peristomial projection (white arrow). J, dorsal infraciliature showing straight dorsal kineties (asterisk). K, detail view showing diplostichomonad paroral (black arrow).Ma, macronucleus.Scale bars: 20 µm (A–E, G–J), 10 µm (F, K).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31921FF9DFEC5FB46962FF8F6.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542003/files/figure.png	https://doi.org/10.5281/zenodo.14542003	Figure 9. Clevelandella hromadkai sp. nov. short morph: from life (A–C, F–H) and after protargol impregnation (D, E, I). From ASS.A, left ventrolateral view showing micronucleus (white arrow), adoral membranelles (black arrow), and the posterior extent of ciliation on the right (black arrowhead) and left (white arrow head) margins. B, ventral view, (optical section) showing the cytostome (white arrowhead), contractile vacuole (black arrow) and margin of the peristomial overture (black arrowhead).C, left lateral view showing collecting vesicles of the contractile vacuole (white arrowheads), the micronucleus (white arrow) and the margin of the peristomial overture (black arrowhead).D,	Figure 9. Clevelandella hromadkai sp. nov. short morph: from life (A–C, F–H) and after protargol impregnation (D, E, I). From ASS.A, left ventrolateral view showing micronucleus (white arrow), adoral membranelles (black arrow), and the posterior extent of ciliation on the right (black arrowhead) and left (white arrow head) margins. B, ventral view, (optical section) showing the cytostome (white arrowhead), contractile vacuole (black arrow) and margin of the peristomial overture (black arrowhead).C, left lateral view showing collecting vesicles of the contractile vacuole (white arrowheads), the micronucleus (white arrow) and the margin of the peristomial overture (black arrowhead).D,	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31921FF9DFEEAF8E19097FD36.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542015/files/figure.png	https://doi.org/10.5281/zenodo.14542015	Figure 13. Clevelandella klobasa sp. nov.in vivo (A–C) and after protargol impregnation (D). From STH. A, left ventrolateral view showing thick hyaline subcortical layer (white arrowheads), cilia of adoral membranelles (black arrow), and margin of the peristomial overture (black arrowhead). B, left lateral view showing thick hyaline subcortical layer (white arrowheads), contractile vacuole (black arrowhead), and micronucleus (white arrow).C, optical section showing adoral membranelles (black arrow) and contractile vacuole (black arrowhead).D, left ventrolateral view (optical section) showing micronucleus (white arrow), adoral membranelles (black arrowhead), and paroral membrane (black arrow).Ma, macronucleus.Scale bars: 20 µm.	Figure 13. Clevelandella klobasa sp. nov.in vivo (A–C) and after protargol impregnation (D). From STH. A, left ventrolateral view showing thick hyaline subcortical layer (white arrowheads), cilia of adoral membranelles (black arrow), and margin of the peristomial overture (black arrowhead). B, left lateral view showing thick hyaline subcortical layer (white arrowheads), contractile vacuole (black arrowhead), and micronucleus (white arrow).C, optical section showing adoral membranelles (black arrow) and contractile vacuole (black arrowhead).D, left ventrolateral view (optical section) showing micronucleus (white arrow), adoral membranelles (black arrowhead), and paroral membrane (black arrow).Ma, macronucleus.Scale bars: 20 µm.	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31921FF9DFC2AFD219097FAE6.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14541999/files/figure.png	https://doi.org/10.5281/zenodo.14541999	Figure 7. Clevelandella philipi sp. nov., in vivo (A, B) and after protargol impregnation (C–I). From STH (A–G) and ASS (H, I). A, ventral view showing thick, hyaline cortical layer (between black arrowheads), cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B, ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A, B). C, ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D, dorsal view showing infraciliature, ciliated circumperistomial kineties (black arrow), looping kineties of peristomial projection (white arrow), and cytoproct (black arrowhead).E, ventral view showing left-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F, dorsal view showing straight somatic kineties (white asterisk), disordered basal bodies on left protuberance (white arrow), and paroral membrane (black arrowhead). G, dorsal view showing micronucleus (white arrowhead). H, dorsal view showing straight dorsal somatic kineties (asterisk), proximal margin of the peristomial overture (white arrow), and looping kineties of peristomial projection (black arrowhead). I, detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer left file (white arrowhead) of basal bodies. Ma, macronucleus. Scale bars:20 µm (A–H) 10 µm (I).	Figure 7. Clevelandella philipi sp. nov., in vivo (A, B) and after protargol impregnation (C–I). From STH (A–G) and ASS (H, I). A, ventral view showing thick, hyaline cortical layer (between black arrowheads), cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B, ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A, B). C, ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D, dorsal view showing infraciliature, ciliated circumperistomial kineties (black arrow), looping kineties of peristomial projection (white arrow), and cytoproct (black arrowhead).E, ventral view showing left-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F, dorsal view showing straight somatic kineties (white asterisk), disordered basal bodies on left protuberance (white arrow), and paroral membrane (black arrowhead). G, dorsal view showing micronucleus (white arrowhead). H, dorsal view showing straight dorsal somatic kineties (asterisk), proximal margin of the peristomial overture (white arrow), and looping kineties of peristomial projection (black arrowhead). I, detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer left file (white arrowhead) of basal bodies. Ma, macronucleus. Scale bars:20 µm (A–H) 10 µm (I).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
EA5087B31921FF9DFC25FAF19097F86C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/14542024/files/figure.png	https://doi.org/10.5281/zenodo.14542024	Figure 15. Clevelandella sidi sp.nov.in vivo (A, B) and after protargol impregnation (C–H). From SRU (A–H). A, ventral view showing contractile vacuole (white arrowhead) and cilia of adoral membranelles (white arrow). B, ventral view showing contractile vacuole (white arrowhead), adoral membranelles (black arrow), and elevated margin of the cytoproct (white arrow).C–E, the same cell in dorsal view: C, showing somatic infraciliature (asterisk), cytoproct (black arrowhead), and adoral membranelles (white arrow); D, showing cytoproct (black arrowhead) and dorsal kineties (white arrowhead); E, optical section showing excretory antrum within the left protuberance (black arrowheads). F, detail of the macronucleus showing the karyophore (white arrow). G, detail showing the micronucleus (white arrowhead). H, detail of the paroral membrane showing both files of equal length and extending nearly to the posterior end of the peristomial projection (white arrowheads), a feature unique to this species.Asterisk marks the anterior end. Ma, macronucleus.Scale bars:20 µm (A–D) 10 µm (E–G), 5 µm (H, I).	Figure 15. Clevelandella sidi sp.nov.in vivo (A, B) and after protargol impregnation (C–H). From SRU (A–H). A, ventral view showing contractile vacuole (white arrowhead) and cilia of adoral membranelles (white arrow). B, ventral view showing contractile vacuole (white arrowhead), adoral membranelles (black arrow), and elevated margin of the cytoproct (white arrow).C–E, the same cell in dorsal view: C, showing somatic infraciliature (asterisk), cytoproct (black arrowhead), and adoral membranelles (white arrow); D, showing cytoproct (black arrowhead) and dorsal kineties (white arrowhead); E, optical section showing excretory antrum within the left protuberance (black arrowheads). F, detail of the macronucleus showing the karyophore (white arrow). G, detail showing the micronucleus (white arrowhead). H, detail of the paroral membrane showing both files of equal length and extending nearly to the posterior end of the peristomial projection (white arrowheads), a feature unique to this species.Asterisk marks the anterior end. Ma, macronucleus.Scale bars:20 µm (A–D) 10 µm (E–G), 5 µm (H, I).	2023-11-14	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan		Zenodo	biologists	Kotyk, Michael;Bourland, William A.;Soviš, Matyáš;Méndez-Sánchez, Daniel;Škaloud, Pavel;Varadínová, Zuzana Kotyková;Čepička, Ivan			
