taxonID	type	description	language	source
E8273615FFC5EF6BFF18FD8CFF14623D.taxon	description	http: // www. zoobank. org / NomenclaturalActs / 4 D 24 EABE- 41 BE- 44 E 5 - 8 AE 6 - AC 27 CD 421 D 19 Doryctobracon sp. 1: Deus et al. 2010 (Pedra Branca do Amapari, AP, ex A. atrigona in Geissospermum argenteum).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC5EF6BFF18FD8CFF14623D.taxon	diagnosis	Diagnosis. This species differs from Doryctobracon areolatus (Szépligeti, 1911), by having the fore wing infuscate with a hyaline band near the base (restricted to the subbasal and anal cell) and a second transverse hyaline band on the apical half (Figs 1 B 1, 3 A). Stigma yellow, veins and bristles in the hyaline band yellow, veins and setae in the infuscate band dark brown (Fig 3 A). Doryctobracon adaimei sp. nov. also has a hyaline area, but it is rounded and is situated after the stigma (dark brown) and before the wing apex (in the middle of R 1 a). This spot does not reach the posterior margin of the wing and ends in the middle of the second subdiscal cell (2 nd disc) (Fig 3 B). In general, D. whartoni sp. nov. is more setose than D. areolatus and D. adaimei sp. nov. (Figs 1 B, 4 A – D). On the propodeum, laterally the anterior region of the areola, one short basal transverse keel projects to reach the lateral longitudinal keel, a characteristic also found in D. adaimei sp. nov. (Fig 4 D); in D. areolatus two transverse keels reach the lateral longitudinal keel. Tegula yellowish orange (dark brown in D. areolatus). Ovipositor apex with a dorsal node as in D. areolatus, but ventral serrations are much more developed (Fig 4 E).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC5EF6BFF18FD8CFF14623D.taxon	description	Description Female. Length of body, excluding ovipositor 6.0 – 6.9 mm. Head. 1.5 – 1.3 × wider than long; 1.4 – 1.3 × wider than width of mesoscutum; midridge polished, slightly elevated, narrow between toruli and wider toward clypeus; distance between toruli equal to distance from toruli to eye (Fig 4 A). Head smooth and polished, with more hairiness concentrated in the clypeus, inner margins of eyes and gena. Antenna slightly longer than body, 7.7 – 9.5 mm in length, with 58 to 63 flagellomeres; first flagellomere about 0.9 – 1.2 × longer than second; 1.7 – 1.9 × longer than wide. Eyes large, 1.3 – 1.4 × wider than high (Fig 4 C); in dorsal view, eye width 1.7 – 1.9 × greater than width of temples; malar space 0.3 – 0.4 × height of eyes. Clypeus 2.9 – 3.8 × wider than high, sinuate, distinctly protruding with median lobe on ventral margin, polished, with sparse setae two to three times longer than those on face (Fig 4 A). Mesosoma. 1.4 – 1.5 × longer than high; 1.9 – 2.1 × longer than wide; 1.3 - 1.5 × higher than wide. Pronotum not visible dorsally; median lobe of mesoscutum and lateral lobes smooth and bright, with few sparse bristles, setae concentrated mainly on margins of lateral lobes (Fig 4 B); notaulus smooth, complete, deeper anteriorly, shallower posteriorly, reunited in a large and polished impression without midpit (Fig 4 B); scutellar sulcus divided into two large pits by median longitudinal septum; scutellum smooth with small punctures and many setae around margins, posterior portion narrower or sharper than in D. adaimei sp. nov. and in D. areolatus (Fig 4 B); propodeum densely setose with median anterior basal keel (0.11 – 0.15) and complete posterior areola (Fig 4 D); laterally, in the anterior region of the areola, one short basal transverse keel extends to reach the lateral longitudinal keel, which is prominent and distinctly curved, from the posterior half of the propodeum (Fig 4 D). Wings. Fore wing 5.8 – 6.4 mm length; wide stigma 3.8 – 4.2 × longer than wide, with vein r slightly projecting from its midpoint; (RS + M) a straight, 1.4 – 1.7 × longer than 3 RSa; 2 RS 1.2 – 1.7 × longer than 3 RSa, 1.3 – 1.5 × longer than 1 m-cu and 1.7 – 2.0 × longer than r-m; 3 RSa 2.3 – 3.3 × longer than vein r; 2 M 1.8 – 2.3 × longer than 3 RSa; (RS + M) b absent; vein 1 cu-a slightly sloping away from 1 M for 0.23 – 0.27 its length. Fore wing infumate with hyaline stripes at base and in median portion. Stigma, veins and setae in hyaline stripe yellow, and veins and setae on infumate stripe dark brown. Hind wing 4.0 – 4.4 mm length, infumate. Metasoma. 2.1 – 2.7 × longer than wide and 1.0 – 1.4 × wider than high; T 1 0.8 – 1.0 × width at apex; T 1 width at apex about 1.2 – 1.4 × width at base; non-sculptured, smooth and bright; ovipositor about 6.0 mm long (twice metasoma length); ovipositor with one subapical dorsal node and developed ventral serrations (Fig 4 E); ovipositor sheath with 4 – 5 rows of bristles (Fig 4 F). General coloration. Yellowish orange; first two pairs of legs bright yellow, third pair with femur, trochanter, and coxa yellow, tibia and tarsi dark brown; ovipositor yellow; antenna and ovipositor sheath dark brown; apex of mandibles black; fore wing infuscate with hyaline stripe, stigma yellow; veins and setae yellow in hyaline stripe, and dark brown in infuscate stripe (Figs 1 B 1, 3 A). Hind wings infumate, sometimes with hyaline stripes at the apex (Fig 1 B 1). Male. Similar to female, but dark brown on last tergite. Head, in dorsal view, 1.23 – 1.30 × wider than mesoscutum, 1.49 – 1.58 × wider than face. In dorsal view, eye 1.71 – 1.74 × wider than temple; face 1.6 – 2.0 × wider than high; malar space 0.40 – 0.48 × height of eyes; clypeus 2.7 – 3.1 × wider than high; antenna with 52 to 59 flagellomeres; first flagellomere 1.0 – 1.2 × longer than second, 1.5 – 1.9 × longer than wide. Mesosoma 1.9 – 2.1 × longer than wide, 1.3 – 1.5 × higher than wide; metasoma 2.0 – 3.0 × longer than wide, 1.0 – 1.5 × wider than high.	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC5EF6BFF18FD8CFF14623D.taxon	materials_examined	Type material. Holotype. Female (DCBU 270194), BRAZIL: Amapá, Pedra Branca do Amapari, 00 ° 46 ’ 54.9 ” N and 051 ° 57 ’ 01.2 ” W, 01. ii. 2006, reared from fruit fly larva Anastrepha atrigona in “ Quina ” (Geissospermum argenteum), coll R. A. Silva. Paratypes, with same data as holotype, 1 female, (DCBU 270195), 2 males (DCBU 270196, DCBU 270197) and 1 female, 1 male (ESALQ).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC5EF6BFF18FD8CFF14623D.taxon	etymology	Etymology. This species is named after Robert A. Wharton, in recognition of his outstanding contributions to the taxonomy of Braconidae. GenBank accession numbers. Doryctobracon whartoni sp. nov. Amapá: FJ 560534 (ITS 2) and FJ 560542 (28 SD 2).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC7EF67FF18FB7FFC8761C7.taxon	description	http: // www. zoobank. org / NomenclaturalActs / 7 E 53462 B- 1 C 9 E- 4 FEC-A 205 - 4 D 9877675 D 4 A	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC7EF67FF18FB7FFC8761C7.taxon	description	Description Female. Length of body, excluding ovipositor 5.5 – 6.3 mm. Head. 1.4 – 1.2 × wider than long; 1.5 – 1.3 × wider than width of mesoscutum. Face polished, bright, and distinctly setose; midridge smoother, restricted between toruli (Fig 5 A); distance between toruli equal to distance from toruli to eye (Fig 5 A). Antenna longer than body, 8.4 – 8.7 mm in length, with 53 to 55 flagellomeres, first flagellomeres about 1.1 – 1.2 × longer than second; 1.5 – 2.1 × longer than wide. Eye large, 1.2 – 1.3 × wider than high (Fig 5 C); in dorsal view, eye 1.6 – 2.0 × wider than temples; malar space 0.4 – 0.5 × longer than height of eyes. Clypeus 2.6 – 3.9 × wider than high, polished, slightly convex and lateral margin slightly curved with sparse setae two to three times longer than setae on face (Fig 5 A); clypeus sinuate, similar to D. whartoni sp. nov., protruding as lobe medially on ventral margin; labrum partially covered by clypeus; distinct opening between clypeus and mandibles (Fig 5 A). Mesosoma. 1.2 – 1.5 × longer than high; 1.7 – 2.0 × longer than wide; 1.3 – 1.5 × higher than wide; pronotum not visible dorsally; median lobe of mesoscutum and lateral lobes as in D. whartoni sp. nov., polished, shiny with few sparse setae; margins of lateral lobes with few setae and shorter than D. whartoni sp. nov. (Fig 5 B); notaulus smooth, complete, deeper anterior to margin of mesoscutum and later shallower, reunited in broad and polished impression without midpit (Fig 5 B); scutellar groove divided into two large pits by median longitudinal septum; scutellum smooth with few or weak punctures, few setae on margins, robust or wide in apical portion, similar to D. areolatus (Fig 5 B); mesopleuron smooth; propodeum setose with median anterior basal keel (0.11 – 0.15) and complete posterior areola. As in D. whartoni sp. nov., laterally, in the anterior region of the areola, one short basal transverse keel extends to reach the lateral longitudinal keel, which is prominent and distinctly curved, from the posterior half of the propodeum (Fig 5 D). Wings. Fore wing 5.3 – 6.3 mm length; stigma wide, 3.0 – 4.2 × longer than wide, with vein r slightly projecting from midpoint; (RS + M) a slightly sinuate posteriorly, 1.0 – 1.6 × longer than 3 RSa; 2 RS 0.7 – 1.3 × longer than 3 RSa, 1.2 – 1.5 × longer than 1 m-cu and 1.6 – 2.6 × longer than r-m; 3 RSa 1.9 – 3.0 × longer than r; 3 RSb ending almost at wing tip; 2 M 1.6 – 1.9 × longer than 3 RSa; (RS + M) b absent; 1 cu-a straight line separated from 1 M by 0.17 – 0.30 in length. Fore wing with a rounded hyaline spot that begins from immediately after stigma and ends in the middle of R 1 a, and before the wing posterior margin at second subdiscal cell (2 nd disc). Length of hind wing 3.4 – 5.0 mm with m-cu, curved, distinctly pigmented just beyond the half toward to the wing margin. Metasoma. 1.2 – 2.3 × longer than wide and 1.0 – 2.3 × wider than high; T 1 length 0.9 – 1.0 mm greater than apex width; metasoma not sculptured, smooth and bright; T 1 apex about 1.2 – 1.4 × width of base; T 1 with two dorsal keels developed at the base that gradually decrease from the posterior half, becoming weak or indistinct (Fig 5 E); spiracles in half of T 1 modified in two oval structures that protrude from the smooth surface, with or without setae (Fig 5 E, F); ovipositor about 5.5 – 6.3 mm long; ovipositor tip with a dorsal node and ventral serrations that are less developed than in D. whartoni sp. nov. (Fig 5 G); ovipositor sheath twice metasoma length, with 4 – 5 rows of setae (Fig 5 H). General coloration. Yellowish orange; first and second pair of legs bright yellow, third pair quite variable, may have all segments with blackish or dark-brown spots (Fig 1 C 2); tibiae and tarsi brown (Fig 1 D 2) or coxa, trochanters, and femur yellow (Fig 1 E 2); tegula yellowish orange; ovipositor sheath and antenna dark brown; apices of mandibles black; T 2, T 3, and T 4 may have black stripes; wings infumate; fore wing with broad rounded hyaline spot that begins from immediately after stigma to middle of R 1 a and in middle of second subdiscal cell (2 nd disc). Veins and setae yellow in the hyaline spot; stigma, veins, and bristles dark brown in infumate area (Fig 3 B). Male. Similar to females, but usually last tergite with dark-brown or black spots. Head in dorsal view 1.7 × wider than mesoscutum width, 2.0 × wider than face; in dorsal view, eye 1.85 × wider than temple; face 1.5 – 1.7 × wider than high; malar space 0.42 – 0.44 × height of eyes; clypeus 2.9 – 3.2 × wider than high; antennae with 50 to 51 flagellomeres; first flagellomere 1.1 - 1.2 × longer than second, 1.8 – 1.9 × longer than wide; mesosoma 1.9 × longer than wide, 1.3 – 1.4 × higher than wide; metasoma 2.4 – 2.7 × longer than wide, 1.1 – 1.4 wider than high.	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC7EF67FF18FB7FFC8761C7.taxon	materials_examined	Type material. Holotype. Female (DCBU 270198), BRAZIL: Amapá, Serra do Navio, 00 ° 55 ’ 57.0 ” N, and 051 ° 55 ’ 14.8 ” W 01. ii. 2006, reared from fruit fly larva in fruits of guava (Psidium guajava), collr. R. A. Silva. Paratypes, with same data as holotype, 2 females (DCBU 270199, DCBU 270200), 1 male (DCBU 270201), collr. R. A. Silva and 1 female (ESALQ); 1 male, Tocantins, 28. ii. 2005, reared from fruit fly larva Anastrepha coronilli in “ Goiaba-de-anta ” (Bellucia grossularioides L.), collr. M. A. Uchôa-Fernandes; 2 females, 1 male, Goiás, Leopoldo Bulhões, 14. ix. 1999, reared from fruit fly larva Anastrepha spp. In “ bacupari ” (Salacia crassifolia) collr. V. R. S Veloso, (ESALQ).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC7EF67FF18FB7FFC8761C7.taxon	etymology	Etymology. This species is named after Ricardo Adaime, who has provided valuable information of many Anastrepha species, their host fruits, and parasitoids in Amapá, Brazil. GenBank accession numbers. Doryctobracon adaimei sp. nov. Amapá, FJ 560535 (ITS 2); Tocantins, FJ 560536 (ITS 2); Goiás, FJ 560537 (ITS 2); Amapá, FJ 560543 (28 SD 2); Tocantins, FJ 560544 (28 SD 2); Goiás, FJ 560545 (28 SD 2).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
E8273615FFC7EF67FF18FB7FFC8761C7.taxon	description	Morphometry. Geometric morphometry generated 36 relative deformation measurements (k = 2 n – 4), where k represents the total number of relative deformations, and n the number of anatomical markers (20). The results of the multivariate analysis (CVA) of populations of male and female D. areolatus, D. whartoni sp. nov. and D. adaimei sp. nov. were statistically significant, Wilks’ Lambda (p <0.0001), Pillai’s Trace (p <0.0001), Hotelling- Lawley Trace (p <0.0001), and Roy’s Greatest Root (p <0.0001). The first two canonical axes explained 66.0 % and 15.7 % of the data variability, respectively. In the scatter plot, we observed the complete separation of the population groups in the space of canonical variables CV 1 and CV 2 (Fig 6). Specimens termed D. whartoni sp. nov., collected only in the state of Amapá, formed an isolated group of D. adaimei sp. nov. and of D. areolatus. Doryctobracon adaimei sp. nov. separated completely from the groups formed by the other species and revealed total overlap of their populations collected in the states of Goiás and Amapá; among the populations of D. areolatus, there was also the formation of distinct grouping with D. whartoni sp. nov. and D. adaimei sp. nov., and total overlap of their populations in the states of Amapá, Tocantins, Goiás and São Paulo (Fig 6). The Mahalanobis distance matrix revealed a lower degree of morphological similarity for D. whartoni sp. nov. in relation to the distinct populations of D. areolatus and D. adaimei sp. nov. We also observed the same result for population of D. adaimei sp. nov. in relation to the populations of D. areolatus; however, the Mahalanobis distances between samples of D. whartoni sp. nov. and D. adaimei sp. nov. were fairly wide, i. e. D. whartoni sp. nov. (AP) - D. adaimei sp. nov. (AP) = 38.39 %, and D. whartoni sp. nov. (AP) - D. adaimei sp. nov. (GO) = 34.96 % (Fig 7, Table 2). Molecular markers. We detected intraspecific size variation for both molecular markers tested when comparing populations of D. areolatus from different geographical regions. The size variation was larger for the ITS 2, which ranged from 564 bp for D. areolatus from the state of Amapá to 590 bp for specimens from the state of São Paulo. ITS 2 was 587 bp long in samples from the states of Goiás and Tocantins. The size of 28 S-D 2 from samples of D. areolatus differed by no more than 5 bp in length, ranging from 381 bp for specimens from Amapá to 386 bp for those from Goiás. Specimens from Tocantins and São Paulo had intermediate sizes (384 and 385 bp, respectively). Despite the variation in size, these markers shared very high sequence similarities (Table 3). However, no size variation was observed for both markers for the species identified as D. whartoni sp. nov. and D. adaimei sp. nov. ITS 2 for D. whartoni sp. nov. from Amapá was 585 bp long, while ITS 2 for D. adaimei sp. nov. from Amapá, Tocantins, and Goiás was 567 bp in length. The 28 S-D 2 of D. whartoni sp. nov. from Amapá was 385 bp long, and 386 (Amapá and Tocantins) and 387 bp long (Goiás) for specimens of D. adaimei sp. nov. Pairwise sequence similarities of ITS 2 and 28 S-D 2 among D. whartoni sp. nov., D. adaimei sp. nov., and D. areolatus were very low (Table 3). Phylogenetic reconstruction of specimens of Doryctobracon species based on the isolated and concatenated sequences of the molecular markers ITS 2 and 28 S-D 2, using maximum likelihood (ML) and neighbor-joining (NJ) methods, produced trees sharing similar topologies and well-defined clades (Fig 8). In all cases, molecular analysis consistently indicated variation in D. areolatus from different collection sites (Amapá, Goiás, Tocantins, and São Paulo), with specimens from Amapá being the most divergent. The most variation in clade definition between the ML and NJ methods was found for the positioning of D. areolatus from Amapá and D. whartoni sp. nov. (Fig 8). Nevertheless, the topologies produced with the concatenated sequences left no doubts regarding the external grouping of specimens of D. areolatus from Amapá from a more internal clade of D. areolatus from the other states. In the concatenated analysis, D. whartoni sp. nov. resolved in a subclade with the divergent D. areolatus from Amapá, but was positioned on a long branch away from D. areolatus (Fig 8 C). The remaining samples of D. areolatus grouped together in a more internal subclade (Fig 8). Samples of D. adaimei sp. nov. always resolved in a defined clade, in the analysis with either the isolated or concatenated molecular markers. In the analysis with the concatenated sequences, the clade of D. adaimei sp. nov. was the first to branch out from all of the remaining samples. The NJ analysis resolved specimens belonging to D. adaimei sp. nov. from Amapá and Tocantins in a more internal subclade from specimens from Goiás, while ML placed specimens from all three populations in a single clade separated from each other by short branches (Fig 8).	en	Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria, Zucchi, Roberto A. (2017): Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses. Zootaxa 4353 (3): 467-484, DOI: 10.11646/zootaxa.4353.3.4
