Tubakia dryinoides C. Nakash., Fungal Systematics and Evolution 1: 80 (2018)

Fig. 5

Description.

Asexual morph: Living as endophyte in leaves, forming distinct leaf lesions, shape and size variable, subcircular to angular-irregular, pale brown to brown. Colonies on PDA incubated at 25°C in the dark with an average radial growth rate of 5-7 mm/d and occupying an entire 90 mm Petri dish in 14 d, forming some conspicuous concentric circles, aerial mycelium cottony, white initially, then becoming greyish-sepia. Conidiomata sporodochial, appeared within 14 days or longer, formed on agar surface, slimy, black, semi-submerged. Sporodochial conidiophores densely and irregularly branched, 11.0-24.0 μm × 1.5-5.0 μm, bearing apical whorls of 2-3 phialides; sporodochial phialides monophialidic, subulate to subcylindrical, 9.0-16.0 μm × 1.5-5.0 μm, smooth, thin-walled, apex obtuse to truncate, sometimes forming indistinct periclinal thickenings. Conidia solitary, ellipsoid to obovoid, 6.5-14.0 μm × 4.0-6.0 μm, wall thin, up to 1.0 μm, hyaline to subhyaline, smooth, apex and base broadly rounded, with inconspicuous to conspicuous basal hilum (frill), occasionally somewhat peg-like and truncate when conspicuous. Microconidia not observed. Sexual morph not observed.

Culture characteristics.

Cultures incubated on MEA at 25°C in darkness, attaining 38.0-42.0 mm diam. after 14 d (growth rate 2.7-3.0 mm diam./d), margin scalloped, at first creamy white, grey near the centre, reverse light brown to dark, with olivaceous edge. Conidial formation not observed.

Specimen examined.

China, Shandong Province: Zibo Lushan National Forest Park, on diseased leaves of Quercus palustris ( Fagaceae), 20 Sep 2020, Z. X. Zhang, HSAUP1924, living culture SAUCC 1924.

Notes.

Braun et al. (2018) described Tubakia dryinoides, based on morphological and molecular data. The holotype of T. dryinoides (NBRC H-11618) was collected from Quercus phillyraeoides A. Gray (Braun et al. 2018). In our current research, isolate (SAUCC 1924) collected from diseased leaves of Quercus palustris clustered in the Tubakia dryinoides clade by strong support (Figs 1 and 2). We, therefore, consider the isolated strain (SAUCC 1924) as T. dryinoides . The conidiomata of T. dryinoides is only known from true pycnothyria and the sporodochial conidiomata of the isolated strain (SAUCC 1924) is new for T. dryinoides (Braun et al. 2018). Additionally, the conidia of our isolate (SAUCC 1924) is narrower than the original description of T. dryinoides (4.0-6.0 μm vs. 5.5-10.0 μm; Braun et al. 2018).